响应面分析法优化乙酸乙酯萃取番茄红素条件的研究

生物技术通报 ›› 2014, Vol. 0 ›› Issue (1): 83-92.

响应面分析法优化乙酸乙酯萃取番茄红素条件的研究

沈海涛,王爱英,祝建波

石河子大学生命科学学院石河子大学农业生物技术重点实验室，石河子 832003

收稿日期:2013-09-06 出版日期:2014-01-23 发布日期:2014-01-23
作者简介:沈海涛，男，硕士，助理研究员，研究方向: 植物基因工程；E-mail: ghost521@126.com
基金资助:
转基因动植物新品种国家重大专项(2011zx08011-002)，石河子大学科学技术研究发展计划(2012ZRKXYQ-YD15)

Research on the Method of Extraction of Lycopeneprepared by Optimizated Ethanol Using Response Surface Analysis

Shen Haitao, Wang Aiying, Zhu Jianbo

College of Life Sciences of Shihezi University; Key Agriculture Biotechnology of Shihezi University，Shihezi 832003

Received:2013-09-06 Published:2014-01-23 Online:2014-01-23

摘要/Abstract

摘要： 采用响应面方法对番茄酱提取番茄红素过程中的乙醇预处理方法、萃取剂萃取时间等工艺条件进行了优化。采用#br#Central Composite Design(CCD) 设计法，对超声波提取法和微波提取法中的乙醇预处理、萃取剂萃取时间、超声波或微波萃取功率#br#、#br#溶剂量4 个因素对番茄红素提取率的影响进行评价。结果显示，最佳提取方法为超声波提取法，无水乙醇∶番茄酱的2.05∶1(V /#br#W)；乙酸乙酯∶番茄酱10.1∶1(V / W)；提取时间为490 s ；超声波提取功率为405 W ；提取率为94.42% 。微波提取最佳方法#br#为，#br#无水乙醇∶番茄酱的2.11∶1(V / W)；乙酸乙酯∶番茄酱10.1∶1(V / W)；提取时间为372.6 s ；微波提取功率为569.5 W ；#br#提取#br#率为81.51% 。

关键词: 番茄酱, 番茄红素, 微波处理, 超声波处理

Abstract: To research the methond of extraction of lycopene repared by optimizated ethanol using response analysis. The effects of ethanol pretreatment, extracting solvent, microwave assisted extraction and ultrasonic assisted extraction, were evaluated by using a central composite rotatable design. Using the dehydration processing tomato sauce can obviously improve the extraction efficiency of Lycopene. Then,the central composite design and response surface analysis were used to determine the optimal levels of the main factors. The optimal extracting conditions and lycopene yields of ultrasound assisted extraction technology were described as follows: the extraction time, 490 s ；the ratio of solvent to tomato sauce, 10.1: 1(V/W)；the ratio of anhydrous ethanol to tomato sauce, 2.05: 1(V/W)； extraction power, 405 W ； lycopene yields, 94.42%. The optimal extracting conditions and lycopene yields of microwave assisted extraction technology were described as follows: the extraction time, 372.6 s ；the ratio of solvent to tomato sauce, 10.1: 1(V/W)；the ratio of ethanol to tomato sauce, 2.11: 1(V/W)； extraction power, 569.5 W ；lycopene yields, 81.51%. Results showed that pretreated by ethanol, power, extracting time and extracting solvent were the main affecting factors.

Key words: Tomato sauce, Lycopene, Microwave treatment, Ultrasonic treatment

沈海涛,王爱英,祝建波. 响应面分析法优化乙酸乙酯萃取番茄红素条件的研究[J]. 生物技术通报, 2014, 0(1): 83-92.

Shen Haitao, Wang Aiying, Zhu Jianbo. Research on the Method of Extraction of Lycopeneprepared by Optimizated Ethanol Using Response Surface Analysis[J]. Biotechnology Bulletin, 2014, 0(1): 83-92.

参考文献

[1] Rao AV, Agarwal S. Role of lycopene as antioxidant carotenoid in the prevention of chronic diseases[J]. Nutrition Research, 1999, 19 (2): 305-323.
[2] Bramley PM. Is lycopene beneficial to human health?[J]. Phytochemistry,2000, 54: 233-236.
[3] Baysal T, Ersus S, Starmans DAJ. Supercritical CO2 extraction of beta-carotene and lycopene from tomato sauce waste[J]. J Agric Food Chem, 2000, 48: 5507-5511.
[4] Dominguez H, Navez MJ, Lama JM. Enzymatic pre-treatment to 图18 提取功率与乙醇预处理体积对番茄红素提取效率影响曲面图 enhance oil extraction from fruits and oil seeds: a review[J].Food Chemistry, 1994, 49: 271-286.
[5] Cadoni E, De Giorgi R, Medda E, Poma G. Supercritical CO2 Extraction of Lycopene and a-Carotene from Tomatoes[J]. Dyes Pigm, 2000, 44: 27-32.
[6] Ciurlia L, Bleve M, Rescio L. Supercritical carbon dioxide coextraction of tomatoes(Lycopersicum esculentum L.)and hazelnuts (Corylus avellana L.): A new procedure in ob-taining a source of natural lycopene[J]. J of Supercritical Fluids, 2009, 49: 338344.
[7] Salisova M, Toma S, Masor TJ. Comparison of conventional and ultrasonically assisted extractions of pharmaceutically active compounds from Salvia officinalis[J]. Ultrasonics Sonochemistry,1997, 4(2): 131-134.
[8] Stanisavljevic’IT, Lazic’M.L, Veljkovic VB. Ultrasonic extraction of oil from tobacco(Nicotiana tabacum L.)seeds[J]. Ultrasonics Sonochemistry, 2007, 14(5): 646-652.
[9] Rodrigues S, Pinto GA, Fernandes FA. Optimization of ultrasound extraction of phenolic compounds from coconut(Cocos nucifera) shell powder by response surface methodology[J].Ultrasonics Sonochemistry, 2008, 15: 95-100.
[10] Gergely A. Csiktusna’di Kissa, Esther Forga’cs, Tibor Cserha’ti. Optimization of the microwave-assisted extraction of pigments from paprika(Capsicum annuum L.)powders[J] ,Journal of Chromatography A, 2000, 889(1-2): 41-49.
[11] Bonrath W. Chemical reactions under“non-classical conditions”,microwaves and ultrasound in the synthesis of vitamins[J].Ultrasonics Sonochemistry, 2004, 11: 1-4.
[12] Hoang TH, Sharma R, Susanto D, et al. Microwave-assisted extraction of active pharmaceutical ingredient from tomato sauce dosage forms[J]. Journal of Chromatography A, 2007, 1156: 149-153.
[13] Proestos C, Komaitis M. Application of microwaveassisted extraction to the extraction of plant phenolic compounds[J]. LWTFood Science and Technology, 2008, 41(4): 652-659.
[14] Craft NE, Soares JH. Relative solubility, stability, and absorptivity of lutein and.beta.-carotene in organic solvents[J]. J Agric Food Chem, 1992, 40: 431-434.
[15] Ishida BK, Chapman MH. Carotenoid extraction from plants using a novel, environmentally friendly solvent[J]. J Agric Food Chem,2009, 57: 1051-10598.
[16] Salari H, Rohani H, Elahifard MR, et al. Solvents Design on the basis of molecular-microscopic properties of binary mixtures for lycopene extraction[J]. American Journal of Chemistry, 2012, 2 (2): 94-98.
[17] Lambelet P, Richelle M, Bortlik K, et al. Improving the stability of lycopene Z-isomers in isomerised tomato extracts[J]. J Food Chemistry, 2009, 112: 156-161.
[18] 王燕, 刘绍, 刘焱, 等. 番茄红素最佳提取方法的研究[J].食品与机械, 2005, 21(1): 17-20.
[19] 姜雨, 赵广华, 胡小松, 等. 乙醇处理对番茄红素提取的影响[J]. 食品科技, 2004, 8: 48-50.
[20] Xu TY. Preparation of high purity lycopene[J]. Fine Chemicals,2006, 23: 62-65.
[21] Zhang LF, Liu ZL. Optimization and comparison of ultrasound/ microwave assisted extraction(UMAE)and ultrasonic assisted extraction(UAE)of lycopene from tomatoes[J]. Ultrasonics Sonochemistry, 2008, 15: 731-737.