哺乳动物正呼肠孤病毒反向遗传学研究进展

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 15-19.

哺乳动物正呼肠孤病毒反向遗传学研究进展

柯飞王赟侯丽芳胡兴安李佩佩

(河南城建学院生命科学与工程学院，平顶山 467036)

收稿日期:2013-11-15 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:柯飞，男，博士，讲师，研究方向：水产动物病毒及免疫；E-mail：530072436@qq.com
基金资助:
国家自然科学青年基金项目(31302227)

Developments in Mammalian Orthoreovirus Reverse Genetics Research

Ke Fei Wang Yun Hou Lifang Hu Xing'an Li Peipei

(College of Life Sciences and Engineering，Henan University of Urban Construction，Pingdingshan 467036)

Received:2013-11-15 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 呼肠孤病毒科是分布最广的病毒类群之一，其中哺乳动物正呼肠孤病毒(Mammalian Orthoreovirus，MRV)属于正呼肠孤病毒属，主要感染哺乳动物的呼吸道和肠道。近年来，MRV的反向遗传学操作技术取得了长足进步，运用该技术，在MRV基因组组装、基因功能及致病机制等方面都获得了较大突破。综述了MRV反向遗传学操作技术的建立过程及运用该技术取得的最新成果，并展望了该技术在呼肠孤病毒科其他病毒中的应用。

关键词: 哺乳动物正呼肠孤病毒, 反向遗传学, 基因功能, 致病机制

Abstract: One of the most widely distributed virus groups is Reoviridae. Mammalian orthoreovirus(MRV)is belonged to the orthoreovirus, which mainly infected the respiratory and gastrointestinal tracts of virtually all mammals, including humans. The MRV reverse genetics have improved significantly in recent years. There are several progresses in MRV genome packaging, gene function and pathogenesis based on this technology. Recent advances on the establishment of MRV reverse genetics and its application are discussed in the text.

Key words: Mammalian orthoreovirus, Reverse, Genetics, Gene function, Pathogenesis

柯飞, 王赟, 侯丽芳, 胡兴安, 李佩佩. 哺乳动物正呼肠孤病毒反向遗传学研究进展[J]. 生物技术通报, 2014, 0(5): 15-19.

Ke Fei, Wang Yun, Hou Lifan,g Hu Xing'an, Li Peipei. Developments in Mammalian Orthoreovirus Reverse Genetics Research[J]. Biotechnology Bulletin, 2014, 0(5): 15-19.

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