生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 57-61.

• 技术与方法 • 上一篇    下一篇

利用SSR技术分析我国16种甜玉米的遗传特性

黄华如 梁凯光 蚁珩鑫 梁雪莲   

  1. (仲恺农业工程学院生命科学学院,广州 510225)
  • 收稿日期:2013-11-18 出版日期:2014-05-23 发布日期:2014-05-24
  • 作者简介:黄华如,男,本科,研究方向:农业生物技术;E-mail:1458486811@qq.com
  • 基金资助:
    广东省大学生创新实验项目(1134711009)

Genetic Characteristics of 16 Kinds of Sweet Corn in China by SSR Analysis

Huang Huaru Liang Kaiguang Yi Hengxin Liang Xuelian   

  1. (College of Life Science,Zhongkai University of Agriculture and Engineering,Guangzhou 510225)
  • Received:2013-11-18 Published:2014-05-23 Online:2014-05-24

摘要: 利用SSR分子标记研究16个甜玉米品种的遗传变异。从200对SSR引物中筛选出10对具有稳定多态性的引物,它们在供试材料中共检测出254个位点,每对引物检测位点5-38个,平均为25.4个,得出的平均多态性信息量为0.920 8;据此进行了聚类分析,将16个品种分为3大类。研究表明,SSR分子标记技术可以用于品种间的遗传分析,方便杂交育种品种的选择。

关键词: 甜玉米, SSR分子标记, 聚类分析

Abstract: SSR markers were used to study the genetic variation of 16 varieties of sweet corn. First of all, 10 primers were screened with stable polymorphism from the 200 pairs of SSR primers;By the 10 primers, 254 allelic variations were detected in the material, each primer pair with 5 to 38 allele average. The average information quantity of polymorphism was 0.920 8. Accordingly, 16 varieties were divided into three broad categories. The study showed that SSR markers can be used to genetic analysis and heterosis breeding.

Key words: Sweet, Corn, SSR, Narker, Clustering analysis