拟南芥原生质体瞬时表达快速验证重组酶外源基因删除

生物技术通报 ›› 2014, Vol. 0 ›› Issue (7): 86-92.

拟南芥原生质体瞬时表达快速验证重组酶外源基因删除

池俊杰^{1, 2}, 戴绍军¹, 魏建华², 王宏芝²

1.东北林业大学盐碱地生物资源环境研究中心东北油田盐碱植被恢复与重建教育部重点实验室, 哈尔滨 150040；
2.北京农业生物技术研究中心, 北京 100097

收稿日期:2014-01-27 出版日期:2014-07-15 发布日期:2014-07-16
作者简介:池俊杰, 男, 硕士, 研究方向：次生细胞壁合成转录调控；E-mail：cjj_2337@163.com
基金资助:
国家高技术研究发展计划（2013AA102702）

Recombinase-mediated Marker Gene Excision in Transient Gene Expression System of Mesophyll Protoplasts

Chi Junjie^{1, 2}, Dai Shaojun¹, Wei Jianhua², Wang Hongzhi²

1. Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040；
2. Beijing Agro-Biotechnology Research Center, Beijing 100097

Received:2014-01-27 Published:2014-07-15 Online:2014-07-16

摘要/Abstract

摘要： 通过在拟南芥原生质体中瞬时表达重组酶删除系统, 以拟南芥热激蛋白Hsp18.2基因的启动子诱导加入马铃薯StLs1基因第2个内含子的重组酶表达, 证明瞬时转化质粒DNA中位于识别位点之间的基因元件可以被有效删除。建立了一种快速验证重组酶外源基因删除系统的方法。

关键词: 位点特异性重组系统, 热激蛋白Hsp18.2启动子, 拟南芥原生质体瞬时表达

Abstract: In the present study, we used transient gene expression system of Arabidopsis mesophyll protoplasts to test and compare site-specific recombination constructs, which the expression of recombinase was induced by the promoter for heat-shock proteins Hsp18.2, developed a simple and convenient system for rapid assessment of site-specific recombination constructs.

池俊杰, 戴绍军, 魏建华, 王宏芝. 拟南芥原生质体瞬时表达快速验证重组酶外源基因删除[J]. 生物技术通报, 2014, 0(7): 86-92.

Chi Junjie, Dai Shaojun, Wei Jianhua, Wang Hongzhi. Recombinase-mediated Marker Gene Excision in Transient Gene Expression System of Mesophyll Protoplasts[J]. Biotechnology Bulletin, 2014, 0(7): 86-92.

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