铝胁迫对转C4 磷酸烯醇式丙酮酸羧化酶基因水稻光合作用和活性氧代谢的影响

生物技术通报 ›› 2014, Vol. 0 ›› Issue (7): 81-85.

铝胁迫对转C4 磷酸烯醇式丙酮酸羧化酶基因水稻光合作用和活性氧代谢的影响

张云华¹, 孟庆玲^{1, 2}, 计成林¹, 陈丽娟¹, 吴港¹, 刘学诗¹, 张署香¹

1.安徽农业大学生命科学学院, 合肥 230036；
2.青岛市崂山区第二中学, 青岛 266000

收稿日期:2014-01-14 出版日期:2014-07-15 发布日期:2014-07-16
作者简介:张云华, 男, 博士, 副教授, 研究方向：生理生态；E-mail：yunhua9681@163.com
基金资助:
科技部星火计划项目（2013GA710066）, 国家自然科学基金项目（31101746）

Effects of Aluminum Stress on Photosynthesis and Active Oxygen Metabolism in C4 Ppc Transgenic Rice

Zhang Yunhua¹, Meng Qingling^{1, 2}, Ji Chenglin¹, Chen Lijuan¹, Wu Gang¹, Liu Xueshi¹, Zhang Shuxiang¹

1. School of Life Science, Anhui Agriculture University, Hefei 230036；
2. Laoshan District of Qingdao No 2 High School, Qingdao 266000

Received:2014-01-14 Published:2014-07-15 Online:2014-07-16

摘要/Abstract

摘要： 以转C4 Ppc基因水稻为材料, 利用叶绿素荧光技术和酶学手段, 调查铝胁迫对转C4 Ppc基因水稻光合作用和活性氧代谢的影响。结果表明, 在铝胁迫条件下, 转C4 Ppc基因水稻的叶绿素含量、光合速率、叶绿素荧光参数Fv/Fm、φPSⅡ、qP和qN下降幅度显著小于野生型。可见在铝胁迫的条件下, 转C4 Ppc基因水稻仍表现出较高的光合效率。铝处理导致水稻活性氧含量的增加, 但由于转C4 Ppc基因水稻活性氧清除酶SOD、POD和CAT含量显著高于野生型, 表现出较低的膜脂过氧化。上述结果表明, C4 Ppc基因的导入显著提高了水稻的耐铝特性。

关键词: 转C4, Ppc基因水稻, 光合作用, 叶绿素荧光, 活性氧代谢

Abstract: The effects of aluminum stress on photosynthesis and active oxygen metabolism were investigated by chlorophyll fluorescence and enzymatic technology in C4 Ppc transgenic rice. The results showed that under Al stress, chlorophyll content, photosynthetic rate and chlorophyll fluorescence parameters Fv/Fm, φPS Ⅱ, qP and qN in C4 Ppc transgenic rice declined significantly less than those in the wild type. The results indicated that C4 Ppc transgenic rice still showed higher photosynthetic efficiency under Al stress compared to the wild type. Al treatment led to an increase in active oxygen content in rice. However, the active oxygen scavenging enzymes such as SOD, POD and CAT in C4 Ppc transgenic rice were significantly higher than those in the wild type, revealing the lipid peroxidation was lower in C4 Ppc transgenic rice. The results indicated that the introduction of C4 Ppc into rice significantly enhanced aluminum tolerance.

Key words: C4, Ppc transgenic rice, Photosynthesis, Chlorophyll fluorescence, Active oxygen metabolism

张云华, 孟庆玲, 计成林, 陈丽娟, 吴港, 刘学诗, 张署香. 铝胁迫对转C4 磷酸烯醇式丙酮酸羧化酶基因水稻光合作用和活性氧代谢的影响[J]. 生物技术通报, 2014, 0(7): 81-85.

Zhang Yunhua, Meng Qingling, Ji Chenglin, Chen Lijuan, Wu Gang, Liu Xueshi, Zhang Shuxiang. Effects of Aluminum Stress on Photosynthesis and Active Oxygen Metabolism in C4 Ppc Transgenic Rice[J]. Biotechnology Bulletin, 2014, 0(7): 81-85.

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