病毒诱导的基因沉默及其在植物研究中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.018

摘要/Abstract

摘要： 病毒诱导的基因沉默（Virus-induced gene silencing, VIGS）在植物的基因功能鉴定中是一个非常有用的工具, 在植物中了得到广泛应用。与传统的研究植物基因阻断的方法相比, VIGS具有简便高效、周期性短、不需要对植物进行转化以及可以沉默基因家族等特点, 而且在一定条件下其沉默效应还能够传递给后代。介绍了VIGS的原理与操作过程, 综述了该技术的应用与研究成果, 有助于推动其在植物尤其是农作物育种中的进一步应用与发展。

关键词: 病毒诱导的基因沉默, 基因功能鉴定

Abstract: Virus-induced gene silencing（VIGS）is a useful tool for the identification of gene function in plants, therefore widely utilized in plants. Compared to traditional gene disruption approaches, VIGS is simple, rapid, short-cycle, transformation-free and capable of silencing multiple gene family members. Moreover, under the certain conditions, VIGS can even be transmitted to progeny plants. Here, the principle and operation of VIGS are introduced, and its recent applications and research advances are reviewed, which conducively promotes its further application and development in plants, especially for crop breeding.

Key words: virus-induced gene silencing, identification of gene function

孙威,许奕,许桂莺,孙佩光,宋顺,常胜合^{. 病毒诱导的基因沉默及其在植物研究中的应用[J]. 生物技术通报, 2015, 31(10): 105-110.}

Sun Wei, Xu Yi, Xu Guiying, Sun Peiguang, Song Shun, Chang Shenghe. Virus-induced Gene Silencing and Its Application in Plant Research[J]. Biotechnology Bulletin, 2015, 31(10): 105-110.

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