解淀粉芽孢杆菌LJ1摇瓶发酵条件优化

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.031

摘要/Abstract

摘要： 解淀粉芽孢杆菌(Bacillus amyloliquefaciens)LJ1是从天津土壤中分离筛选出的一株具有广谱抑菌效果的生防菌株，该实验旨在明确B. amyloliquefaciens LJ1的抑菌谱并通过优化发酵培养基和发酵条件提升其抑菌效果。通过对峙培养检测菌株LJ1对14种病原真菌的抑制效果；采用单因素试验、正交试验优化发酵培养基和摇瓶发酵条件；最后用孢子萌发法和盆栽试验验证优化后发酵液的抑菌效果。实验结果表明B. amyloliquefaciens LJ1对黄瓜灰霉病菌(Botrytis cinerea)、苹果炭疽病菌(Glomerella cingulata)、苹果腐烂病菌(Valsa ceratosperma)等11种病原真菌有抑制作用；优化后的摇瓶发酵条件为温度30℃、初始pH 5、转速200 r/min、装液量50 mL/250 mL；优化后的发酵培养基为马铃薯 200 g，蔗糖10 g，黄豆粉20 g，MgCl₂·6H₂O 1.5 g，蒸馏水 1 000 mL。经发酵条件与培养基优化后，B. amyloliquefaciens LJ1发酵液对Botrytis cinerea孢子萌发的抑制率达99.7%，盆栽实验防效达51.08%。

关键词: 解淀粉芽孢杆菌LJ1, 抑菌谱, 发酵条件优化

Abstract: Bacillus amyloliquefaciens strain LJ1 isolated from the soil of Tianjin is biocontrol one with broad inhibition spectrum. This study aims to clarify the inhibition spectrum of strain B. amyloliquefaciens LJ1 and improve the antifungal activity by optimizing the medium composition and fermentation conditions. The confront culture was performed to test the antifungal activity of LJ1 on 14 phytopathogenic fungi. The medium composition and fermentation conditions were optimized by single-factor and orthogonal experiments. Finally, the inhibitory effect of optimized broth was tested by methods of spore germination and pot experiment. The results showed that B. amyloliquefaciens LJ1 inhibited 11 tested fungal pathogens, i. e. , Botrytis cinerea, Glomerella cingulata, and Valsa ceratosperma. The optimal fermentation conditions were as temperature 30℃, initial pH5.0, rotation speed 200 r/min, and medium volume 50 mL/250 mL. The optimal composition of the medium was 200 g potato, 10 g sucrose, 20 g soybean flour, 1.5 g MgCl₂·6H₂O, and 1 000 mL distilled water. In conclusion, the inhibition rate of B. amyloliquefaciens LJ1 to the conidia germination of B. cinerea reached 99.7%, and the control efficacy of pot experiment was 51.08% under the optimum fermentation medium and culture conditions.

Key words: Bacillus amyloliquefaciens LJ1, inhibition spectrum, optimization of fermentation condition

李娟,夏凯丽,王远宏,董蕴琪,郝蕾. 解淀粉芽孢杆菌LJ1摇瓶发酵条件优化[J]. 生物技术通报, 2015, 31(12): 214-220.

Li Juan, Xia Kaili, Wang Yuanhong, Dong Yunqi, Hao Lei. The Optimization of Fermentation Condition in Flask for Bacillus amyloliquefaciens LJ1[J]. Biotechnology Bulletin, 2015, 31(12): 214-220.

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