'易丰'女贞组培快繁技术体系的建立

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.014

生物技术通报 ›› 2015, Vol. 31 ›› Issue (12): 97-104.doi: 10.13560/j.cnki.biotech.bull.1985.2015.12.014

'易丰'女贞组培快繁技术体系的建立

孙英坤¹,代其林²,张俊林¹,王劲³,沈柏春¹,陈林敬¹,钱飞¹,吴光洪¹

1.杭州市园林绿化股份有限公司，杭州 310000；2.西南科技大学生命科学与工程学院，绵阳 621010；3.中国农业科学院生物技术研究所，北京 100081

收稿日期:2015-03-25 出版日期:2015-12-19 发布日期:2015-12-19
作者简介:孙英坤，男，工程师, 硕士研究生，研究方向:园林植物组培快繁；E-mail:sunyingkun4@163.com
基金资助:
国家重点基础研究发展计划(2013CB733903)，国家高技术研究发展计划(2012AA063503)，农业部公益性行业科研专项(201103007)

The Establishment of Tissue Culture and Rapid Propagation System of Ligustrum lucidum 'Yi Feng'

Sun Yingkun¹, Dai Qilin², Zhang Junlin¹, Wang Jin³, Shen Bochun¹, Chen Linjing¹, Qian Fei¹, Wu Guanghong¹

1. Hangzhou Landscaping Incorporated Company，Hangzhou 310000；2. College of Life Science and Technology，Southwest University of Science and Technology，Mianyang 621010；3. Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081

Received:2015-03-25 Published:2015-12-19 Online:2015-12-19

摘要/Abstract

摘要： 以'易丰'女贞(Ligustrum lucidum'Yi Feng')当年新发半木质化带腋芽茎段为外植体，以芽繁芽的方式，建立了较为完善的可用于大规模工厂化生产的组培快繁技术体系。研究表明，最佳诱芽(启动)培养基为WPM+0.3 mg/L Kinetin(KT)+0.1 mg/L 6-Benzylaminopurine(6-BA)+0.05 mg/L 1-Naphthylacetic acid(NAA)，腋芽萌发率达到95.6%；最佳的增殖培养基为WPM+0.5 mg/L KT +1.0 mg/L 6-BA +0.05 mg/L NAA，平均增殖系数达到5.63；最佳的生根培养基为1/2WPM+1.5 mg/L 3-Indolebutyric acid(IBA)+0.1 mg/L NAA，生根周期约为20 d，生根率约为95.4%，炼苗移栽后成活率达到95%以上。建立的组培技术体系能够满足产业化生产的需要，得到的组培苗后代能够稳定地遗传亲本的优良变异性状。

关键词: 易丰'女贞, 快速繁殖, 增殖培养

Abstract: Ligustrum lucidum'Yi Feng', a wild and variant species, because its resource was very scarce, and routine methods could not achieve large-scale propagation during a short period of time, a relatively perfect tissue culture and rapid propagation system for massive production was established in this research with axillary buds of young branch cuttings as explant materials. The results showed that, the optimal initial medium was WPM+0.3 mg/L KT +0.1 mg/L 6-BA +0.05 mg/LNAA, and the germination frequency of axillary buds reached 95.6%；the optimal proliferation medium was WPM+0.5 mg/L KT +1.0 mg/L 6-BA +0.05 mg/L NAA, and its average proliferation coefficient reached 5.63；the optimal rooting medium of shoots was 1/2WPM+1.5 mg/L IBA +0.1 mg/L NAA. After about 20 days, the overall frequency of shoots rooting reached about 95.4%. And then, more than 95% of the plantlets could survive on the substrate soil in the end. The system can satisfy the need of industrial production, and the plantlets can steadily inherit the outstanding variation character of parents.

Key words: Ligustrum lucidum 'Yi Feng', rapid propagation, proliferation culture

孙英坤,代其林,张俊林,王劲,沈柏春,陈林敬,钱飞,吴光洪. '易丰'女贞组培快繁技术体系的建立[J]. 生物技术通报, 2015, 31(12): 97-104.

Sun Yingkun, Dai Qilin, Zhang Junlin, Wang Jin, Shen Bochun, Chen Linjing, Qian Fei, Wu Guanghong. The Establishment of Tissue Culture and Rapid Propagation System of Ligustrum lucidum 'Yi Feng'[J]. Biotechnology Bulletin, 2015, 31(12): 97-104.

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'易丰'女贞组培快繁技术体系的建立

The Establishment of Tissue Culture and Rapid Propagation System of Ligustrum lucidum 'Yi Feng'

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