关键词: 纳米碳, 组织培养, 增殖, 褐化

Abstract: This work is to study the effects of different concentrations of nano carbon on growth and differentiation of several plants’ explants. The test tube seedling of edible lily,Anthurium andraeanum,Cymbidium hybridum,and Jinchang jujube as experimental material was cultured on MS medium with different concentrations of nano carbon. Results showed that,MS + 6-BA 0.8 mg/L + NAA 0.2 mg/L + nano carbon 0.10 g/L was conducive to the multiplication of lily bulb with the multiplication coefficient 2.11;when the concentration of nano carbon was 0.50 g/L,the growth and rooting of lily in vitro was significantly better. The optimal medium for induction and differentiation of Anthurium ‘s axillary buds was MS+ 6-BA 1.5 mg/L + IBA 0.2 mg/L + nano carbon 0.04 to 0.06 g/L,the induction rate was over 90% and the differentiation rate was 97%. The addition of nano carbon 1.0 to 2.0 g/L obviously inhibited the browning of C. hybridum protocorm subculture. The optimal medium for the multiplication cultivation of Jinchang jujube was MS + 6-BA 1.5 mg/L + IBA 0.5 mg/L + nano carbon 0.05 g/L,the multiplication coefficient reached 9.76;bud growth was robust when the concentration of nano carbon was 0.15 g/L.

Key words: nano carbon, tissue culture, multiplication, browning