酵母抽提物对CHO细胞生长及抗体表达的影响

doi:10.13560/j.cnki.biotech.bull.1985.2016-1020

摘要/Abstract

摘要： 为了深入认识酵母抽提物在中国仓鼠卵巢（CHO）细胞生长及单克隆抗体表达过程中所发挥的作用,综合考察了传代培养和批式培养过程中,不同浓度酵母抽提物条件下CHO细胞生长、抗体表达以及营养物代谢的情况。传代培养过程中,低浓度（1 g/L）的酵母抽提物能够显著促进CHO细胞的生长,高浓度（5-10 g/L）的酵母抽提物则会显著抑制CHO细胞的生长;同时,传代过程中添加酵母抽提物不会影响种子细胞在批式培养中的表现。批式培养过程中,抗体比生成速率随酵母抽提物浓度的提高而升高,浓度为10 g/L时获得最高抗体产量。通过采用细胞生长阶段低浓度、产物表达阶段高浓度的添加策略,酵母抽提物在动物细胞培养过程中可发挥巨大的应用价值。

关键词: 动物细胞培养, CHO细胞, 酵母抽提物, 单克隆抗体, 氨基酸代谢

Abstract: The objective is to get insights into the effects of yeast extract on Chinese hamster ovary（CHO）cell growth and monoclonal antibody production. We comprehensively investigated the cell growth,antibody production and nutrients metabolism at different yeast extract concentrations in continuous passage culture and batch culture. It was found that yeast extract significantly improved CHO cell growth at low concentration（1 g/L）and remarkably inhibited CHO cell growth at high concentration（5-10 g/L）during continuous passage culture. Additionally,the result of seed cells in batch culture was not influenced while adding the yeast extract during continuous passage culture. Supplementing yeast extract in batch culture medium,a positive correlation was found between specific antibody production rate and the yeast extract concentration with a maximal antibody titer achieved at yeast extract concentrations of 10 g/L. In conclusion,yeast extracts can make a great contribution on animal cell culture process with a strategy of low concentration added at cell growth phase and high concentration added at protein-production phase.

Key words: animal cell culture, CHO cells, yeast extract, monoclonal antibody, amino acid metabolism

胡冬冬, 赵亮, 范里, 刘旭平, 邓献存, 缪仕伟, 谭文松. 酵母抽提物对CHO细胞生长及抗体表达的影响[J]. 生物技术通报, 2017, 33(6): 162-169.

HU Dong-dong, ZHAO Liang, FAN Li, LIU Xu-ping, DENG Xian-cun, MIU Shi-wei, TAN Wen-song. Effects of Yeast Extract on Cell Growth and Antibody Production in CHO Cell Culture[J]. Biotechnology Bulletin, 2017, 33(6): 162-169.

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