基于质谱的定量蛋白质组学技术发展现状

doi:10.13560/j.cnki.biotech.bull.1985.2017-0343

摘要/Abstract

摘要： 蛋白质组定量分析技术是支撑蛋白质组学研究的关键技术之一,随着蛋白质组定量分析技术的发展,基于质谱的定量蛋白质组学已成为蛋白质组学研究的重要分支。蛋白质组学定量技术可分为非靶向定量和靶向定量两类,靶向定量技术有MRM和PRM模式,非靶向定量技术有非标记定量和体内外标记定量模式,目前使用最多的同位素标记试剂是iTRAQ和TMT。蛋白质组定量技术按数据采集模式还可分DDA和DIA两类。通过对国内外相关文献收集和分析,系统介绍了蛋白质组质谱定量技术的主要特点和发展现状,旨在为生命科学研究者更好地应用定量蛋白质组学技术提供帮助。

关键词: 定量蛋白质组学, 靶向定量, 稳定同位素标记, 非标记定量, 翻译后修饰定量

Abstract: Quantitative proteomic analysis is one of the key technologies in proteomics research. With the development of quantitative techniques,quantitative proteomics based on mass spectrometry has become an important branch of proteomics research. Quantitative proteomics technology is generally classified into two categories,untargeted quantitative proteomics and targeted quantitative proteomics. Targeted quantitative technology includes MRM and PRM models,and untargeted quantitative technology includes label-free and in vivo or vitro labeling. The most commonly used isotope labeling reagents are iTRAQ and TMT. According to the data collection model,the quantitative technology can be grouped into DDA and DIA. Through the collection and analysis of relevant literature,this article systematically introduces the main characteristics and current situation of quantitative proteomics based on mass spectrometry,aiming at providing help for the researchers on life science to better apply the quantitative proteomics technologies.

Key words: quantitative proteomics, target quantitative, stable isotope labeling, label-free, post-translational modification

牟永莹,顾培明,马博,闫文秀,王道平,潘映红. 基于质谱的定量蛋白质组学技术发展现状[J]. 生物技术通报, 2017, 33(9): 73-84.

MU Yong-ying,GU Pei-ming,MA Bo,YAN Wen-xiu,WANG Dao-ping,PAN Ying-hong. Advancements in Quantitative Proteomics Technologies Based on Mass Spectrometry[J]. Biotechnology Bulletin, 2017, 33(9): 73-84.

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