生物技术通报 ›› 2018, Vol. 34 ›› Issue (12): 96-101.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0446

• 研究报告 • 上一篇    下一篇

水稻籼、粳花粉源基因飘流的竞争性

赵建发1, 何光亮1, 何美丹1, 柯智1, 翟楠鑫1, 符亚小宁1, 裴新梧2, 袁潜华1   

  1. 1. 海南大学热带农林学院,海口 570228;
    2. 中国农业科学院生物技术研究所 农业部转基因安全评价重点实验室,北京 100081
  • 收稿日期:2018-05-14 出版日期:2018-12-26 发布日期:2018-12-24
  • 作者简介:赵建发,男,硕士研究生,研究方向:植物生物技术与种质创新;E-mail:2395016127@qq.com
  • 基金资助:
    国家自然科学基金项目(31260153,31560188),转基因重大专项(2016ZX08011-001)

The Competitiveness of Transgene Flow Between Indica and Japonica Rice Pollen Donor

ZHAO Jian-fa1, HE Guang-liang1, HE Mei-dan1, KE Zhi1, ZHAI Nan-xin1, FU Ya-xiaoning1, PEI Xin-wu2, YUAN Qian-hua1   

  1. 1. Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;
    2. MOA Key Laboratory on Safety Assessment(Molecular)of Agri-GMO,Chinese Academy of Agricultural Sciences,Beijing 100081
  • Received:2018-05-14 Published:2018-12-26 Online:2018-12-24

摘要: 基因飘流是水稻(Oryza sativa L.)转基因安全性研究中的重要内容,研究花粉竞争对基因飘流的影响对于建立并完善转基因飘流模型具有重要意义。本实验分别以籼、粳型转基因水稻和籼、粳常规水稻为花粉供体,不育系为花粉受体,研究籼、粳型双供体在基因飘流中的竞争性。结果表明,同种亚种双供体的基因飘流率与花粉源数量成正相关关系,且基因飘流率与供体花粉数量关系符合S曲线。基因飘流是花粉数量竞争和遗传竞争的综合结果。

关键词: 转基因水稻, bar基因, 基因飘流, 花粉竞争性

Abstract: Gene flew is an important part of the research on the safety of transgenic rice(Oryza sativa L.). It is of great significance to study the effect of pollen competition on gene flow in establishing and improving the gene flew model. In this experiment,the Japonica /Indica of transgenic rice and the common rice plants were used as pollen donors,and the male sterile line BoA was a pollen receptor. The results showed that the gene flow frequencies of dual donors of the same species was positively correlated with the quantity of pollen sources,and the relationship between the gene flow frequency and the pollen quantity of donor was consistent with S curve. Gene flow is a comprehensive result of pollen quantity competition and genetic competition.

Key words: transgenic rice, bar, gene flew, pollen competition