木薯不同发育期块根基因组DNA甲基化变化分析

doi:10.13560/j.cnki.biotech.bull.1985.2017-0893

生物技术通报 ›› 2018, Vol. 34 ›› Issue (5): 117-123.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0893

木薯不同发育期块根基因组DNA甲基化变化分析

薛晶晶,陈松笔

中国热带农业科学院热带作物品种资源研究所农业部木薯种质资源保护与利用重点实验室,儋州 571737

收稿日期:2017-10-23 出版日期:2018-05-26 发布日期:2018-06-07
作者简介:薛晶晶,女,博士,助理研究员,研究方向：木薯基因克隆和表观遗传学;E-mail：xuetao608@catas.cn
基金资助:
国家自然科学基金国际交流与合作项目（31271776）,中央级公益性科研院所基本科研业务费专项资助项目（1630032013005）

Variation Analysis of DNA Methylation in Different Development Stages of Cassava

XUE Jing-jing, CHEN Song-bi

Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Conservation and Utilization for Cassava Germplasm Resources,Danzhou 571737

Received:2017-10-23 Published:2018-05-26 Online:2018-06-07

摘要/Abstract

摘要： DNA甲基化在植物的转座子沉默和基因表达调控中起着重要的作用。本研究以淀粉含量差异较大的两个木薯种质SC5和Cas36-12为研究材料,采用甲基化敏感扩增多态性（MSAP）技术,通过LabChip GX Touch微流控毛细管电泳系统分析其块根发育过程中的甲基化变化情况。结果表明：14对选择性扩增引物在SC5和Cas36-12中分别扩增出345和 339个甲基化条带,SC5三个发育期的甲基化率高于Cas36-12,且都超过50%。比较SC5和Cas36-12块根发育3个关键时期的甲基化变化情况,发现两份材料甲基化变化的整体趋势是一致的。形成期到膨大期主要以发生甲基化为主;而膨大期到成熟期主要以去甲基化为主。

关键词: SC5, Cas36-12, 储藏根, DNA 甲基化, MSAP分析

Abstract: DNA methylation plays a key role in the regulation of gene expression and the silencing of transposons in plants. In this study,two cassavas’ germplasm SC5 and Cas36-12 with large differences in starch content were used as study material. The methylation-sensitive amplified polymorphism（MSAP）technique was used to analyze the DNA methylation changes of two cassavas’ germplasm in the different development stages by LabChip GX Touch microfluidic capillary electrophoresis. The results showed that 345 and 339 methylation bands were amplified from SC5 and Cas36-12 by 14 pairs of primers,respectively. The methylation rate of SC5 was higher than that of Cas36-12 in the three developmental stages,and all were over 50%. Comparing the DNA methylation changes in the 3 key developmental stages of SC5 and Cas36-12 root tuber,it was found that the overall changing trend of DNA methylation of two materials was consistent. From the formation period to the expansion,the DNA methylation mainly occurred,while the DNA de-methylation mainly occurred from expansion to maturation.

Key words: SC5, Cas36-12, storage root, DNA methylation, MSAP analysis

薛晶晶,陈松笔. 木薯不同发育期块根基因组DNA甲基化变化分析[J]. 生物技术通报, 2018, 34(5): 117-123.

XUE Jing-jing, CHEN Song-bi. Variation Analysis of DNA Methylation in Different Development Stages of Cassava[J]. Biotechnology Bulletin, 2018, 34(5): 117-123.

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木薯不同发育期块根基因组DNA甲基化变化分析

Variation Analysis of DNA Methylation in Different Development Stages of Cassava

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