生物技术通报 ›› 2018, Vol. 34 ›› Issue (7): 81-84.doi: 10.13560/j.cnki.biotech.bull.1985.2017-1001

• 植物激素专题 • 上一篇    下一篇

茉莉酸甲酯对丹参毛状根有效成分含量的影响

熊丙全1, 刘冬青2, 廖相建1, 郑雪莲2   

  1. 1. 成都农业科技职业学院园林园艺分院,成都 611130;
    2. 电子科技大学生命科学与技术学院,成都 610054
  • 收稿日期:2017-11-27 出版日期:2018-07-26 发布日期:2018-08-01
  • 作者简介:熊丙全,男,硕士,副教授/高级农艺师,研究方向:园艺植物及药用植物;E-mail:45973177@qq.com
  • 基金资助:
    四川省教育厅2017年度自然科学项目(17zb0069),中央高校基本科研业务费(zygx2014j081,zygx2016j122),2015年成都农业科技职业学院院级科研课题(cny15-09)

Effects of Methyl Jasmonate on Active Ingredient Content in Hairy Roots of Salvia miltiorrhiza

XIONG Bing-quan1, LIU Dong-qing2, LIAO Xiang-jian1, ZHENG Xue-lian2   

  1. 1. Department of Landscape Gardening,Chengdu Agricultural College,Chengdu 611130;
    2. School of Life Sciences and Technology,University of Electronic Science and Technology of China,Chengdu 610054
  • Received:2017-11-27 Published:2018-07-26 Online:2018-08-01

摘要: 研究茉莉酸甲酯对丹参毛状根中次生代谢产物含量的影响,为丹参毛状根培养的生产应用提供实验依据。以茉莉酸甲酯作为丹参毛状根的诱导子,进行丹参毛状根诱导培养。发现茉莉酸甲酯诱导的丹参毛状根的丹参酮类化合物丹参酮Ⅰ和丹参酮ⅡA分别是无茉莉酸甲酯诱导的1.53倍和1.16倍,酚酸类化合物迷迭香酸和丹酚酸B分别是无茉莉酸甲酯诱导的1.37倍和4.43倍。茉莉酸甲酯能显著提高丹参毛状根次生代谢产物的合成和积累,通过茉莉酸甲酯诱导实现丹参有效次生代谢产物的高效生产。

关键词: 茉莉酸甲酯, 丹参, 毛状根, 有效成分含量

Abstract: The effects of methyl jasmonate(MJ)on the content of secondary metabolites in Salvia miltiorrhiza hairy roots were studied to provide experimental basis for the production and application of culturing hairy roots of S. miltiorrhiza. Having methyl jasmonate as the inducer,the hairy root culture of S. miltiorrhiza was induced. The results showed that the contents of tanshinone Ⅰ and tanshinone ⅡA of S. miltiorrhiza hairy roots with MJ were 1.53 times and 1.16 times higher than those of hairy roots without MJ respectively. Similarly,the contents of rosmarinic acid and salvianolic acid B were 1.37-fold and 4.43-fold increase in S. miltiorrhiza hairy roots with MJ. The results confirmed that MJ was an efficient inducer,which significantly improved the synthesis and accumulation of the secondary metabolites in S. miltiorrhiza hairy roots. In another words,induction by MJ could be used for the efficient production of effective secondary metabolites of S. miltiorrhiza.

Key words: methyl jasmonate, Salvia miltiorrhiza, hairy root, active ingredient content