藏鸡IRX3基因表达与肌内脂肪的沉积关系

doi:10.13560/j.cnki.biotech.bull.1985.2018-0048

摘要/Abstract

摘要： 旨在构建藏鸡IRX3基因的组织及时序表达谱,确定该基因与肌内脂肪的沉积关系。利用荧光定量PCR技术检测IRX3基因在不同组织和不同发育阶段肌肉组织中的表达水平,同时采用单因素方差分析方法分析肌内脂肪含量,并分析其时序表达与肌内脂肪含量的相关性。结果表明,IRX3基因在藏鸡各个组织中均有表达,在藏鸡肺组织中相对表达水平较高,极显著高于其他各个组织（P<0.01）。IRX3 mRNA在210日龄公藏鸡腿肌和胸肌中相对表达水平均极显著高于其他日龄的表达水平（P<0.01）,而在1日龄母藏鸡胸肌中相对表达水平极显著高于其他日龄（P<0.01）。藏鸡不同发育阶段肌肉组织中IRX3 mRNA表达水平与其IMF含量的相关性分析显示,公藏鸡腿肌IRX3 mRNA表达量与肌内脂肪含量呈正相关（P<0.01）,而在母藏鸡胸肌IRX3 mRNA表达量与肌内脂肪含量呈负相关（P<0.01）。IRX3基因表达与藏鸡肌内脂肪含量存在相关性,推测IRX3可能对肌内脂肪的沉积具有调控作用。

关键词: 藏鸡, IRX3, 组织表达, 时序表达, 肌内脂肪

Abstract: The objective of this study is to establish IRX3 gene expression profiles in different tissues and muscle tissues of different developmental stages,and to analyze the correlations between the expression profile of IRX3 gene and the deposition of intramuscular fat in Tibetan chicken. The expression levels of IRX3 gene in different tissues and muscle tissues at different developmental stages were detected by real-time fluorescence quantitative PCR,intramuscular fat contents of the tissues were also measured,and its correlations with the expression level of IRX3 gene were analyzed. The results showed that the expression of IRX3 gene in lung tissue was significantly higher than that in other tissues（P < 0.01）. The relative mRNA levels of IRX3 in the leg and the chest muscle from 210 days-old cock were significantly higher than that of other ages（P < 0.01）,while the relative expression levels of IRX3 mRNA in the chest muscle from one day old hen were significantly higher than that from other ages（P < 0.01）. Correlation analysis showed that expression levels of IRX3 were positively correlated with intramuscular fat content in cock leg muscle（P < 0.01）,while the expression levels were negatively correlated with intramuscular fat content in female chest muscle（P < 0.01）. As conclusion,there is a correlation between IRX3 gene expression and intramuscular fat content,thus it is inferred that IRX3 might regulate intramuscular fat deposition.

Key words: Tibetan chicken, IRX3, tissue expression, developmental expression, IMF

张亚楠, 林亚秋, 许晴, 徐亚欧, 贺庆华. 藏鸡IRX3基因表达与肌内脂肪的沉积关系[J]. 生物技术通报, 2018, 34(9): 219-223.

ZHANG Ya-nan, LIN Ya-qiu, XU Qing, XU Ya-ou, HE Qing-hua. Correlation Analysis Between IRX3 Gene Expression and Intramuscular Fat Deposition in Tibetan Chicken[J]. Biotechnology Bulletin, 2018, 34(9): 219-223.

参考文献

[1] 陈国宏, 王克华, 王金玉, 等. 中国禽类遗传资源[M]. 上海:上海科学技术出版社, 2004.
[2] 强巴央宗, 张浩, 商鹏, 等. 藏鸡屠宰性能及肉质特性[J]. 中国农业大学学报, 2008, 13(1):47-50.
[3] 甘麦邻, 堵晶晶, 杨琼, 等. 动物肌内脂肪对肉质的影响及其分子机制研究进展[J]. 现代畜牧兽医, 2017(10):51-57.
[4] Bellefroid EJ, Kobbe A, Gruss P, et al.Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification[J]. EMBO J, 1998, 17(1):191-203.
[5] Scarlett K, Pattabiraman V, Barnett P, et al.The proangiogenic effect of iroquoishomeobox transcription factor Irx3 in human microvascular endothelial cells[J]. J Biol Chem, 2015, 290(10):6303-6315.
[6] Reggiani L, Raciti D, Airik R, et al.The prepattern transcription factor Irx3 directs nephron segment identity[J]. Genes & Development, 2007, 21(18):2358-2370.
[7] Zhang SS, Kim KH, Rosen A, et al.Iroquois homeobox gene 3 establishes fast conduction in the cardiac His-Purkinje network[J]. Proc Natl Acad Sci USA, 2011, 108(33):13576-13581.
[8] Scott Smemo, Tena JJ, Kim KH, et al.Obesity-associated variants within FTO form long-range functional connections with IRX3[J]. Nature, 2014, 507(7492):371-375.
[9] Huang T, Qi Q, Li Y, Hu FB, et al.FTO genotype, dietary protein, and change in appetite:the preventing overweight using novel dietary strategies trial[J]. American Journal of Clinical Nutrition, 2014, 99(5):1126-1130.
[10] Muc M, Padez C, Manco L.Influence of physical activity on the association between the FTO variant rs9939609 and adiposity in young adults[J]. Am J Hum Biol, 2015, 27(5):734-738.
[11] Zou YY, Lu P, Shi J, Liu W, et al.IRX3 promotes the browning of white adipocytes and its rare variants are associated with human obesity risk[J]. EBioMedicine, 2017, 24:64-75.
[12] Sidossis L, Kajimura S.Brown and beige fat in humans:thermogenic adipocytes that control energy and glucose homeostasis[J]. J Clin Invest, 2015, 125(2):478-486.
[13] Adeyemo A, Chen G, Zhou J, et al.FTO genetic variation and asso-ciation with obesity in West Africans and African Americans[J]. Diabetes, 2010, 59(6):1549-1554.
[14] Claussnitzer M, Dankel SN, Kim KH, et al.FTO obesity variant circuitry and adipocyte browning in humans[J]. N Engl J Med, 2015, 373(10):895-907.
[15] 郭秀兰, 唐仁勇, 刘达玉, 等. 肌内脂肪对猪肉品质的影响及其营养调控[J]. 中国畜牧兽医, 2011, 38(5):214-216.
[16] Houweilin AC, DildropR, Peters T, et, al. Gene and cluster-specific expression of the Iroquois family members during mouse development[J]. Mech, 2001, 107(1):169-175.
[17] Van Tuyl M, Liu J, Groenman F, et al.Iroquois genes influence proximo-distal morphogenesis during rat lung development[J]. American Journal of Physiology. Lung Cellular and Molecular Physiology, 2005, 290(4):L777-L789.
[18] 樊红樱. 呼伦贝尔绵羊尾部脂肪组织的转录组差异表达分析[D]. 兰州:甘肃农业大学, 2016.
[19] Kolatad K.Fat deposition and distribution measured by computer tomography in threegenetic groups of pigs[J]. Livestock Production Science, 2001 67(3):281-292.
[20] 王贝贝, 李琦华, 李玉, 等. 肉鸡肌内脂肪沉积的研究进展[J]. 黑龙江畜牧兽医, 2014(21):65-67.
[21] 聂晓庆, 林亚秋, 徐亚欧, 等. 藏鸡磷酸酪氨酸互作结构域1(PID1)的克隆及组织表达谱研究[J]. 畜牧兽医学报, 2016, 47(6):1102-1111.
[22] 林森, 林亚秋, 朱江江, 等. 藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析[J]. 畜牧兽医学报, 2017, 07(48):1191-1201.