邻苯二甲酸酯类污染物雌激素结合活性筛查

doi:10.13560/j.cnki.biotech.bull.1985.2018-0480

生物技术通报 ›› 2019, Vol. 35 ›› Issue (1): 214-218.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0480

邻苯二甲酸酯类污染物雌激素结合活性筛查

张晓聪^1,2, 张冬生³, 刘兰华¹, 周小红¹, 刘春², 陆韻¹, 施汉昌¹

1. 清华大学环境学院环境模拟与污染控制国家重点实验室,北京 100084;
2. 河北科技大学环境科学与工程学院,石家庄 050018;
3. 河北省食品检验研究院,石家庄 050200

收稿日期:2018-05-24 出版日期:2019-01-26 发布日期:2019-01-23
作者简介:张晓聪,女,硕士研究生,研究方向：环境雌激素筛查技术;E-mail：1151663769@qq.com
基金资助:
北京市自然科学基金项目（8182030）

Screening of Estrogenic Binding Activity of Phthalic Acid Esters

ZHANG Xiao-cong^1,2, ZHANG Dong-sheng³, LIU Lan-hua¹, ZHOU Xiao-hong¹, LIU Chun², LU Yun¹, SHI Han-chang¹

1. State Key Joint Laboratory of ESPC,School of Environment,Tsinghua University,Beijing 100084;
2. School of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018;
3.Hebei Food Inspection and Research Institute,Shijiazhuang 050200

Received:2018-05-24 Published:2019-01-26 Online:2019-01-23

摘要/Abstract

摘要： 以倏逝波光纤荧光生物传感器为平台,以邻苯二甲酸酯类（PAEs）污染物为靶标,优化了基于受体作用理论的生物传感分析技术,实现了PAEs雌激素结合活性的定性筛查。对7种典型邻苯二甲酸酯的雌激素结合活性测试结果为BBP>DBP>DIPP,其他4种物质几乎无雌激素结合活性,结果与文献广泛报道结论相一致,验证了所建立方法的准确性。在最优测试条件下,光纤传感界面可再生300次以上,为雌激素活性污染物的筛查提供了一种低成本、自动化方法。

关键词: 倏逝波光纤荧光生物传感器, 雌激素受体, 邻苯二甲酸酯, 雌激素结合活性

Abstract: Taking the evanescent-wave optical fiber fluorescent biosensor as a platform and the phthalic acid esters（PAEs）contaminants as targets,the biosensing analytical technology based on the estrogen receptor interaction theory was optimized,and qualitative screening of estrogen binding activity of PAEs was achieved. The order of the estrogenic binding activity of seven typical PAEs were BBP > DBP > DIPP. The other 4substances had almost no estrogenic binding activity,which was consistent with the widely reported conclusions in Literatures,and confirming the accuracy of the established method. Under the optimal test conditions,the optical fiber sensing surface can be regenerated more than 300 times,providing a Low-cost,automated method for the screening of estrogenic contaminants.

Key words: evanescent-wave fiber fluorescent biosensor, estrogen receptor, phthalates, estrogenic binding activity

张晓聪, 张冬生, 刘兰华, 周小红, 刘春, 陆韻, 施汉昌. 邻苯二甲酸酯类污染物雌激素结合活性筛查[J]. 生物技术通报, 2019, 35(1): 214-218.

ZHANG Xiao-cong, ZHANG Dong-sheng, LIU Lan-hua, ZHOU Xiao-hong, LIU Chun, LU Yun, SHI Han-chang. Screening of Estrogenic Binding Activity of Phthalic Acid Esters[J]. Biotechnology Bulletin, 2019, 35(1): 214-218.

参考文献

[1] Lyche JL, Gutleb AC, Bergman Å, et al.Reproductive and developmental toxicity of phthalates[J]. Journal of Toxicology & Environmental Health Part B, 2009, 12(4):225-249.
[2] Meeker JD, Sathyanarayana S, Swan SH.Phthalates and other additives in plastics:human exposure and associated health outco-mes[J]. Philos Trans R Soc Lond B Biol Sci, 2009, 64(1526):2097-2113.
[3] EUA. Department of Health and Human Services. Agency for toxic substances and disease registry. Toxicological profile for di-n-octylphthalate[J]. Atsdr Toxicological Profile, 1997.
[4] EUA. Department of Health and Human Services. Agency for toxic substances and disease registry. Toxicological profile for di-n-butyl-phthalate[J]. Atsdr Toxicological Profile, 2001, 18(3):187-191.
[5] Heudorf U, Mersch-Sundermann V, Angerer J.Phthalates:Toxicology and exposure[J]. Int J Hyg Environ Health, 2007, 210(5):623-634.
[6] Jobling S, Reynolds T, White R, et al.A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic[J]. Environ Health Perspect, 1995, 103(6):582-587.
[7] Takeuchi S, Iida M, Kobayashi S, et al.Differential effects of phthalate esters on transcriptional activities via human estrogen receptors α and β, and androgen receptor[J]. Toxicology, 2005, 210(2):223-233.
[8] Carrillo JD, Salazar C, Moreta C, et al.Determination of phthalates in wine by headspace solid-phase microextraction followed by gas chromatography-mass spectrometry:fibre comparison and selection[J]. J Chromatogr A, 2007, 1164(1-2):248-261.
[9] Fang GZ, He JX, Wang S.Multiwalled carbon nanotubes as sorbent for on-Line coupling of solid-phase extraction to high-performance liquid chromatography for simultaneous determination of 10 sulfonamides in eggs and pork[J]. J Chromatogr A, 2006, 1127(1-2):12-17.
[10] Speltini A, Sturini M, Maraschi F, et al.Graphene-derivatized silica as an efficient solid-phase extraction sorbent for pre-concentration of fluoroquinolones from water followed by liquid-chromatography fluorescence detection[J]. J Chromatogr A, 2015, 1379:9-15.
[11] Kuang H, Xu L, Cui G, et al.Development of determination of di-n-octyl phthalate(DOP)residue by an indirect enzyme-Linked immunosorbent assay[J]. Food and Agricultural Immunology, 2010, 21(3):265-277.
[12] Li N, Wang D, Zhou Y, et al.Dibutyl phthalate contributes to the thyroid receptor antagonistic activity in drinking water processes[J]. Environ Sci Technol, 2010, 44(17):6863-6868.
[13] Takenouchi O, Kanno A, Takakura H, et al.A bioluminescent indicator for highly sensitive analysis of estrogenic activity in a cell-based format[J]. Bioconjug Chem, 2016, 27(11):2689.
[14] Liu L, Zhou X, Lu Y, et al.Facile screening of potential xenoestro-gens by an estrogen receptor-based reusable optical biosensor[J]. Biosensors and Bioelectronics, 2017, 97:16-20.

邻苯二甲酸酯类污染物雌激素结合活性筛查

Screening of Estrogenic Binding Activity of Phthalic Acid Esters

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 7

编辑推荐

Metrics

本文评价

[1]	张小红, 陶红, 王亚娟, 李一春, 张锐. 固定化微球降解土壤中PAEs效果及影响因素[J]. 生物技术通报, 2021, 37(1): 223-233.
[2]	乐爱文;单莉莉;袁瑞;董洁;肖天慧;卓蓉;王中海;. 原因不明子宫内膜薄雌激素受体α基因多态性及其表达[J]. , 2010, 0(07): 195-200.
[3]	乐爱文;单莉莉;袁瑞;董洁;肖天慧;卓蓉;王中海;. 原因不明子宫内膜薄雌激素受体β基因RsaⅠ、AluⅠ多态性[J]. , 2009, 0(12): 102-107.
[4]	张伟;时德;杜成友;罗放;. 雌激素受体a基因多态性与原发性肝癌的相关性研究[J]. , 2008, 0(03): 84-86.
[5]	. 国内信息[J]. , 2001, 0(05): 48-52.
[6]	孙国凤. 用aporto调节G-CSF产生的基因治疗技术的开发[J]. , 1996, 0(04): 26-27.
[7]	. 激素和活性多肽[J]. , 1987, 0(04): 89-93.