生物技术通报 ›› 2021, Vol. 37 ›› Issue (1): 223-233.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0675
张小红1(), 陶红1,3(), 王亚娟2, 李一春1, 张锐1
收稿日期:
2020-06-02
出版日期:
2021-01-26
发布日期:
2021-01-15
作者简介:
张小红,女,硕士研究生,研究方向:土壤有机污染与修复;E-mail: 基金资助:
ZHANG Xiao-hong1(), TAO Hong1,3(), WANG Ya-juan2, LI Yi-chun1, ZHANG Rui1
Received:
2020-06-02
Published:
2021-01-26
Online:
2021-01-15
摘要:
旨为研究土壤邻苯二甲酸酯污染修复中,固定化微球降解土壤中邻苯二甲酸酯的效果及影响因素。以海藻酸钠为载体,采用包埋法对课题组前期提取的微小杆菌进行固定化,比较固定化微球和游离菌降解土壤中邻苯二甲酸酯(Phthalates esters,PAEs)的效果及pH、温度、重金属、无机盐等对降解菌降解目标物的影响。结果显示:(1)在土壤环境相同条件下,固定化微球对邻苯二甲酸二甲酯(Dimethyl ortho-phthalate,DMP)、邻苯二甲酸二正丁酯(Di-n-butyl ortho-phthalate,DnBP)和邻苯二甲酸二(2-乙基己)酯(Bis(2-ethylhexyl)ortho-phthalate,DEHP)的降解效果高于游离菌,DMP在7 d可降解完全,DnBP在10 d内可降解完全,DEHP在20 d降解率63.73%;而游离菌则在15 d内完全降解DMP,20 d内完全降解DnBP,DEHP在20 d降解率48.77%;(2)不同pH值时,固定化微球对DMP、DnBP、DEHP的降解率均高于游离菌,pH9时,固定化微球对于DMP、DnBP、DEHP的降解率最高分别为96.81%、89.39%、58.35%;(3)不同温度,固定化微球对DMP、DnBP、DEHP的降解率也均高于游离菌,温度为30℃时,固定化微球对于DMP、DnBP、DEHP的降解效率达到最高,分别为96.27%、89.19%、59.01%;(4)重金属使游离菌对DMP、DnBP、DEHP降解率下降较多,而使固定化微球对DMP、DnBP的降解率仅下降了16.35%、9.95%,DEHP不仅没有降低,反而增加2.49%,说明重金属对游离菌起到很强的抑制作用,但对于固定化微球的降解效果影响较小;(5)盐碱条件下,中性盐极大降低了游离菌和固定化微球降解DMP、DnBP、DEHP的降解能力,碱性盐和混合盐对降解菌影响较小,且增强了固定化微球对DnBP、DEHP的降解能力。固定化微球降解PAEs效果明显高于游离菌,对外界环境有更好的适应能力,且对重金属、无机盐污染环境有一定的抵御能力。
张小红, 陶红, 王亚娟, 李一春, 张锐. 固定化微球降解土壤中PAEs效果及影响因素[J]. 生物技术通报, 2021, 37(1): 223-233.
ZHANG Xiao-hong, TAO Hong, WANG Ya-juan, LI Yi-chun, ZHANG Rui. Effect and Influencing Factors of Immobilized Microspheres on Degradation of Phthalate Esters in Soil[J]. Biotechnology Bulletin, 2021, 37(1): 223-233.
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