生物技术通报 ›› 2020, Vol. 36 ›› Issue (10): 127-134.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0236

• 研究报告 • 上一篇    下一篇

耐盐碱溶磷菌Y2R2的分离鉴定及溶磷特性

杨海霞(), 刘希旻, 潘奕臣, 赵香林, 黄海东()   

  1. 天津农学院 农学与资源环境学院,天津 300384
  • 收稿日期:2020-03-07 出版日期:2020-10-26 发布日期:2020-11-02
  • 作者简介:杨海霞,女,研究方向:生物技术;E-mail: yhx0118@126.com
  • 基金资助:
    国家级大学生创新创业训练计划项目(201910061002);国家自然科学基金面上项目(31571790)

Isolation,Identification and Characterization of Salt-alkali-tolerant and Phosphorus-dissolving Bacterium Y2R2

YANG Hai-xia(), LIU Xi-min, PAN Yi-chen, ZHAO Xiang-lin, HUANG Hai-dong()   

  1. College of Agronomy & Resources and Environment,Tianjin Agricultural University,Tianjin 300384
  • Received:2020-03-07 Published:2020-10-26 Online:2020-11-02

摘要:

为筛选适应滨海盐碱地环境条件的溶磷菌,从天津滨海新区盐碱土壤中分离得到1株耐盐碱溶磷菌Y2R2,多相分类鉴定结果表明其为盐单胞菌属(Halomonas)的一个新种。菌株Y2R2的生长温度范围为10-45℃,能够耐受pH 14和NaCl浓度15%的生长条件。在低于pH 13和NaCl浓度12%的条件下,菌株Y2R2具有较强的溶磷能力,在pH 8和NaCl浓度9%的无机磷培养基中,发酵48 h溶磷能力最高,达到247.6 mg/L。筛选到的菌株Y2R2为适应滨海盐碱地土壤和气候环境的土著微生物新种,具有较高的耐盐碱和溶磷能力,有望用于耐盐碱溶磷菌剂的开发。

关键词: 耐盐碱溶磷菌, 分类鉴定, 盐单胞菌, 新种

Abstract:

The objective of this study is to screen phosphorus-dissolving bacteria with fine environment adaptability to coastal salt-alkali land. Strain Y2R2 with the ability of salt-alkali-tolerant and phosphorus-dissolving was isolated from salt-alkali soil samples collected from Tianjin Binhai New Area. Strain Y2R2 was identified as a novel species of Halomonas according to poly-phase taxonomic characteristics. Strain Y2R2 grew at the temperature 10℃ to 45℃,and tolerated the condition of pH 14 and 15% NaCl. Strain Y2R2 showed ability of dissolving phosphorus under pH 7-13 and NaCl content 0-12%. In inorganic phosphorus medium,under the fermentation conditions in which the initial pH was 8,NaCl content was 9% and fermentation time was 48 h,the phosphate-dissolving quantity by strain Y2R2 reached its highest value,247.6 mg/L. Strain Y2R2 screened in this study is a novel species of indigenous microorganisms that adapted to the soil and climate environment of coastal salt-alkali land,and has high salt-alkali tolerance and phosphate-dissolving capacities. The strain is expected to be used as salt-alkali phosphorus-dissolving bacterial agent.

Key words: salt-alkali-tolerant phosphorus dissolving bacteria, taxonomic identification, Halomonas, novel species