生物技术通报 ›› 2021, Vol. 37 ›› Issue (5): 141-153.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1452

• 综述与专论 • 上一篇    下一篇

植物VIGS技术及其在林业科学中的研究进展

高鹏飞1(), 席飞虎1,2, 张泽宇1, 胡凯强1, 陈凯2, 魏文桃1, 丁家治1, 顾连峰1()   

  1. 1.福建农林大学林学院 基础林学与蛋白质组学中心,福州 350002
    2.福建农林大学生命科学学院,福州 350002
  • 收稿日期:2020-11-27 出版日期:2021-05-26 发布日期:2021-06-11
  • 作者简介:高鹏飞,男,硕士研究生,研究方向:林木遗传育种;E-mail: pfgao925@163.com
  • 基金资助:
    国家自然科学基金项目(31570674);沿海木麻黄良种定向选育技术与应用;福建省林木种苗科技攻关六期项目

Research Progress of Plant VIGS Technology and Its Application in Forestry Science

GAO Peng-fei1(), XI Fei-hu1,2, ZHANG Ze-yu1, HU Kai-qiang1, CHEN Kai2, WEI Wen-tao1, DING Jia-zhi1, GU Lian-feng1()   

  1. 1. College of Forestry,Basic Forestry and Proteomics Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002
    2. College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002
  • Received:2020-11-27 Published:2021-05-26 Online:2021-06-11

摘要:

随着基因组序列信息的激增,生物科学研究进入大数据时代,但如何对基因组信息进行功能注释成为了一个重要的研究目标。病毒诱导的基因沉默(virus-induced gene silencing,VIGS)技术作为一种强大的工具,可以针对缺乏稳定遗传转化体系的林木物种进行基因功能分析,已经被用来研究了多个植物生长发育过程中的关键基因。该技术利用植物先天抗病毒防御机制,通过把目的基因片段插入病毒载体中进而在植物体内产生小干扰RNA,使该植物内源基因的转录物成为被降解的靶标,从而导致目的基因的表达量下调。系统综述了VIGS技术的优势、局限性、以及相应的解决方案,同时还讨论了VIGS在林木科学研究中的应用,最后探究了VIGS作为探究和表征植物基因功能的有力工具的最新改进方案和未来前景。

关键词: 病毒诱导的基因沉默, 功能基因组学, 病毒载体, 林业

Abstract:

With the explosion of genomic sequence information,biological science has entered the era of big data. Functional annotation of genomic information has become an important research goal. Virus-induced gene silencing(VIGS)is a powerful tool in analysis of gene function for forest species that are lack of stable genetic transformation system. VIGS has been used to study multiple key genes in plant growth and development. VIGS works as such way,a fragment of target gene is inserted into a viral vector to generate small interfering RNAs(siRNAs)based on the mechanism of natural innate antiviral defense in plant,then the transcript of endogenous gene in the plant becomes the degraded target,resulting in down-regulation of target gene expressions. Here we systematically review the advantages and limitations of VIGS,as well as corresponding solutions. Meanwhile we also discuss the application of VIGS system in forest science. Finally,we explore the latest improving solutions and future prospects while VIGS as a powerful tool for assessing and characterizing gene functions in plant.

Key words: virus-induced gene silencing, functional genomics, virus vectors, forestry