生物技术通报 ›› 2021, Vol. 37 ›› Issue (7): 88-97.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0508

• 研究报告 • 上一篇    下一篇

线虫效应子MgMO237及互作蛋白OsCRRSP55在水稻中的共响应基因鉴定

李治文1(), 刘培燕1, 陈建松1,2, 廖金铃1,2,3, 林柏荣1,2(), 卓侃1,2()   

  1. 1.华南农业大学植物保护学院植物线虫研究室,广州 510642
    2.广东省微生物信号与作物病害防控重点实验室,广州 510642
    3.广东生态工程职业学院,广州 510520
  • 收稿日期:2021-04-18 出版日期:2021-07-26 发布日期:2021-08-13
  • 作者简介:李治文,男,博士研究生,研究方向:植物与病原物相互作用;E-mail:lizhiwen2017@126.com
  • 基金资助:
    国家自然科学基金项目(31471750);国家自然科学基金项目(31772136);国家自然科学基金项目(31972246);广东省自然科学基金项目(2021A1515010937)

Identification of Rice Genes Responding to Both the Nematode Effector MgMO237 and Its Interacting Protein OsCRRSP55

LI Zhi-wen1(), LIU Pei-yan1, CHEN Jian-song1,2, LIAO Jin-ling1,2,3, LIN Bo-rong1,2(), ZHUO Kan1,2()   

  1. 1. Laboratory of Plant Nematology,College of Plant Protection,South China Agricultural University,Guangzhou 510642
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control,South China Agricultural University,Guangzhou 510642
    3. Guangdong Eco-Engineering Polytechnic,Guangzhou 510520
  • Received:2021-04-18 Published:2021-07-26 Online:2021-08-13

摘要:

前期研究表明拟禾本科根结线虫效应子MgMO237与水稻OsCRRSP55蛋白互作,抑制水稻对根结线虫的防卫反应。植物CRRSPs蛋白参与植物的生物和非生物胁迫响应,因此研究MgMO237与OsCRRSP55相互作用对下游基因表达的影响可为阐明水稻对根结线虫的防卫反应机制提供理论依据。生物信息学分析表明OsCRRSP55蛋白有N端信号肽,没有跨膜结构域,具两个DUF26结构域及一保守基序C-X8-C-X2-C。RT-qPCR分析显示OsCRRSP55在水稻根中表达,且在拟禾本科根结线虫侵染7 d后的根结中表达显著上调。水杨酸和茉莉酸显著提高水稻OsCRRSP55及其启动子结合转录因子OsWRKY47的表达。转录组测序结果显示茉莉酸响应基因OsERF87在MgMO237转基因植株中表达显著下调,而RT-qPCR检测发现OsERF87在OsCRRSP55转化水稻原生质体中表达显著上调,表明茉莉酸响应基因OsERF87是OsCRRSP55和MgMO237的共响应基因。该结果表明拟禾本科根结线虫效应子MgMO237可能通过水稻OsCRRSP55蛋白调控茉莉酸激素信号传导,从而抑制植物对线虫的防卫反应。

关键词: 拟禾本科根结线虫, MgMO237, OsCRRSP55, OsERF87, 茉莉酸信号途径

Abstract:

Previous studies showed that the Meloidogyne graminicola effector MgMO237 may interact with the rice protein OsCRRSP55 and inhibit rice defense responses against M. graminicola. Plant protein CRRSPs are involved in plant responses to both biotic and abiotic stresses. Understanding the influence of the interaction between MgMO237 and OsCRRSP55 on the expressions of their downstream genes will provide a theoretical basis for elucidating the mechanism of rice defense responses against root-knot nematodes. Bioinformatics analysis indicated that the OsCRRSP55 protein contained an N-terminal signal peptide and two conserved domains called the domain unknown function 26(DUF26),in which occurred a conserved motif C-X8-C-X2-C,but had no transmembrane domains. RT-qPCR analysis indicated that the OsCRRSP55 expressed in the rice roots and it was significantly up-regulated in the galls at 7 d infection by M. graminicola compared with that in healthy root tip. The expression levels of OsCRRSP55 and its promoter binding transcription factor OsWRKY47 significantly increased in the rice treated with salicylic acid and methyl jasmonate compared with those in wild-type ones. Transcriptome sequencing showed that a jasmonic acid-responsive gene OsERF87 was down-regulated in MgMO237-transgenic rice,but up-regulated in rice overexpressing OsCRRSP55 by RT-qPCR,showing the OsERF87 was co-regulated by OsCRRSP55 and MgMO237. In sum,these results demonstrate that the M. graminicola effector MgMO237 may affect the jasmonic acid signal transmission by interacting with OsCRRSP55,which results in the suppression of plant defense responses to M. graminicola.

Key words: Meloidogyne graminicola, MgMO237, OsCRRSP55, OsERF87, jasmonate signaling pathway