白芷根际促生菌的筛选及其促生效果研究

doi:10.13560/j.cnki.biotech.bull.1985.2021-1386

摘要/Abstract

摘要：

发掘与利用白芷根际细菌促生潜力,为白芷高产优质提供新途径。使用功能性培养基分离筛选白芷根际中具有较强促生潜力的植物根际促生菌（plant growth promoting rhizobacteria,PGPR）,通过形态特征及16S rDNA序列对菌株进行综合鉴定,采用盆栽试验验证所筛PGPR菌株对BZA001和BZB003两个白芷品种（系）的促生效果。分离出白芷根际细菌共77株,23株具有固氮能力,20株具有溶解无机磷能力,4株具有产铁载体能力,7株具有产IAA能力,所有菌株均无解钾能力;31株促生潜力较强的PGPR多属于芽孢杆菌属（Bacillus）,占64.52%;所有菌株以Klebsiella属XI-1菌株综合促生能力最强,在不施肥条件下该菌株对BZA001和BZB003两个白芷品种（系）的生长及产量均有显著促进作用,增产率分别为31.85%和64.59%,在一定程度上缓解了缺肥对白芷生长的限制。白芷根际促生菌中芽孢杆菌数量最多;但所筛克雷伯氏菌株XI-1综合促生潜力最强,可显著提高白芷产量,具有发展为白芷微生物菌剂的潜质。

关键词: 白芷, 分离筛选, 根际促生菌, 产量

Abstract:

The aim of the study is to explore and utilize the growth promoting potential of rhizosphere bacteria of Angelica dahurica var. formosana and to provide a new way for high yield and quality of A. dahurica var. formosana. Functional media were used to isolate and screen plant growth promoting rhizobacteria（PGPR）in the rhizosphere of A. dahurica var. formosana. Then morphological characteristics and 16S rDNA sequence were to comprehensively identify the PGPR strains. Pot experiment was conducted to verify the effects of the screened strain with the strongest growth promoting ability on BZA001 and BZB003 variety（line）of A. dahurica var. formosana. The results showed that a total of 77 strains of bacteria were isolated,among which 23 strains had the ability of fixing nitrogen,20 strains had the ability to dissolving inorganic phosphorus,4 strains had the ability of producing siderophores,7 strains had the ability of producing IAA,and all strains had no ability of dissolving potassium. Most of the 31 PGPR strains with strong growth promoting ability belonged to Bacillus,accounting for 64.52%. Of all the isolated PGPR strains,Klebsiella strain XI-1 had the strongest comprehensive growth promoting ability. Without fertilization,the 2 strains significantly increased the growth and yield of two A. dahurica var. formosana varieties（line）BZA001 and BZB003 by 31.85% and 64.59%. To a certain extent,it alleviated the restriction of fertilizer deficiency on A. dahurica var. formosana. Most of the PGPR in the rhizosphere of A. dahurica var. formosana were Bacillus. However,Klebsiella XI-1 has the strongest growth promoting potential and may significantly improve the yield of A. dahurica var. formosana. Thus there is the potential of developing it into a microbial agent of A. dahurica var. formosana.

Key words: Angelica dahurica var. formosana, isolation and screening, rhizosphere growth-promoting bacteria, yield

江美彦, 周杨, 刘仁浪, 姚菲, 杨云舒, 侯凯, 冯冬菊, 吴卫. 白芷根际促生菌的筛选及其促生效果研究[J]. 生物技术通报, 2022, 38(8): 167-178.

JIANG Mei-yan, ZHOU Yang, LIU Ren-lang, YAO Fei, YANG Yun-shu, HOU Kai, FENG Dong-ju, WU Wei. Screening and Plant Growth Promoting of Grow-promoting Bacteria in Rhizosphere Bacteria of Angelica dahurica var. formosana[J]. Biotechnology Bulletin, 2022, 38(8): 167-178.

图/表 13

参考文献 40

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菌株编号 Bacterial strain No.	固氮能力 Nitrogen-fixing ability	菌株编号 Bacterial strain No.	固氮能力 Nitrogen-fixing ability	菌株编号 Bacterial strain No.	固氮能力 Nitrogen-fixing ability
XA-4	+	XD-6	+	XD-25	+
XB-8	++	XD-9	++	XD-27	++
XB-9	++	XD-10	++	XD-30	+
XB-11	++	XD-13	++	XD-34	+
XB-13	++	XD-17	+	XD-36	+
XB-14	++	XD-20	+	XI-1	++
XB-15	++	XD-23	++	XI-3	++
XD-2	+	XD-24	+

菌株编号 Bacterial strain No.	固氮能力 Nitrogen-fixing ability	菌株编号 Bacterial strain No.	固氮能力 Nitrogen-fixing ability	菌株编号 Bacterial strain No.	固氮能力 Nitrogen-fixing ability
XA-4	+	XD-6	+	XD-25	+
XB-8	++	XD-9	++	XD-27	++
XB-9	++	XD-10	++	XD-30	+
XB-11	++	XD-13	++	XD-34	+
XB-13	++	XD-17	+	XD-36	+
XB-14	++	XD-20	+	XI-1	++
XB-15	++	XD-23	++	XI-3	++
XD-2	+	XD-24	+

菌株Bacterial strain	颜色Color	形态Morphology	隆起度Uplift	边缘Edge	光泽Glossy	革兰氏染色Gram staining
XA-4	乳白色	圆形	扁平	裂叶状	无	+
XA-6	乳白色	点状	拱起	完整	有	+
XB-8	乳白色	圆形	拱起	波状	有	-
XB-9	乳白色	圆形	扁平	波状	有	-
XB-11	透明	圆形	扁平	波状	有	-
XB-13	乳白色	圆形	扁平	波状	有	-
XB-14	乳白色	圆形	扁平	波状	有	-
XB-15	白色	圆形	扁平	波状	有	+
XC-1	白色	圆形	扁平	波状	有	+
XC-2	白色	圆形	拱起	完整	无	+
XD-2	灰白色	圆形	扁平	波状	有	+
XD-3	白色	圆形	脐突状	裂叶状	无	+
XD-6	白色	圆形	拱起	裂叶状	无	+
XD-9	乳白色	圆形	扁平	波状	有	+
XD-10	灰白色	圆形	扁平	完整	有	+
XD-11	白色	圆形	拱起	完整	有	+
XD-13	灰白色	圆形	扁平	波状	有	+
XD-15	白色	圆形	拱起	裂叶状	无	+
XD-17	灰白色	圆形	扁平	完整	有	+
XD-20	白色	圆形	拱起	波状	有	+
XD-23	白色	圆形	脐凹状	波状	有	+
XD-24	白色	圆形	扁平	裂叶状	无	+
XD-25	灰白色	圆形	脐凹状	波状	有	+
XD-27	白色	圆形	脐凹状	完整	有	+
XD-29	乳白色	不规则	拱起	裂叶状	有	+
XD-30	灰白色	圆形	拱起	波状	有	+
XD-34	白色	圆形	脐凹状	波状	有	+
XD-36	灰白色	圆形	脐凹状	波状	有	+
XI-1	灰白色	圆形	拱起	波状	有	-
XI-3	灰白色	圆形	拱起	波状	有	-
XI-7	黄白色	圆形	扁平	波状	有	+

菌株Bacterial strain	颜色Color	形态Morphology	隆起度Uplift	边缘Edge	光泽Glossy	革兰氏染色Gram staining
XA-4	乳白色	圆形	扁平	裂叶状	无	+
XA-6	乳白色	点状	拱起	完整	有	+
XB-8	乳白色	圆形	拱起	波状	有	-
XB-9	乳白色	圆形	扁平	波状	有	-
XB-11	透明	圆形	扁平	波状	有	-
XB-13	乳白色	圆形	扁平	波状	有	-
XB-14	乳白色	圆形	扁平	波状	有	-
XB-15	白色	圆形	扁平	波状	有	+
XC-1	白色	圆形	扁平	波状	有	+
XC-2	白色	圆形	拱起	完整	无	+
XD-2	灰白色	圆形	扁平	波状	有	+
XD-3	白色	圆形	脐突状	裂叶状	无	+
XD-6	白色	圆形	拱起	裂叶状	无	+
XD-9	乳白色	圆形	扁平	波状	有	+
XD-10	灰白色	圆形	扁平	完整	有	+
XD-11	白色	圆形	拱起	完整	有	+
XD-13	灰白色	圆形	扁平	波状	有	+
XD-15	白色	圆形	拱起	裂叶状	无	+
XD-17	灰白色	圆形	扁平	完整	有	+
XD-20	白色	圆形	拱起	波状	有	+
XD-23	白色	圆形	脐凹状	波状	有	+
XD-24	白色	圆形	扁平	裂叶状	无	+
XD-25	灰白色	圆形	脐凹状	波状	有	+
XD-27	白色	圆形	脐凹状	完整	有	+
XD-29	乳白色	不规则	拱起	裂叶状	有	+
XD-30	灰白色	圆形	拱起	波状	有	+
XD-34	白色	圆形	脐凹状	波状	有	+
XD-36	灰白色	圆形	脐凹状	波状	有	+
XI-1	灰白色	圆形	拱起	波状	有	-
XI-3	灰白色	圆形	拱起	波状	有	-
XI-7	黄白色	圆形	扁平	波状	有	+

菌株Bacterial strain	对比信息（登陆号）Comparison information（Accession）	相似性Similarity /%	系统类别 System class
XA-4	Bacillus subtilis strain ge25（MW186208.1）	99.93	Bacillus
XA-6	Paenibacillus alvei isolate Paenibacillus B-LR1（LS992241.1）	99.93	Paenibacillus
XB-8	Enterobacter cloacae strain NaCd1（KT336353.1）	99.79	Enterobacter
XB-9	Enterobacter cloacae strain NaCd1（KT336353.1）	99.79	Enterobacter
XB-11	Pseudomonas lalkuanensis strain PE08（CP043311.1）	99.93	Pseudomonas
XB-13	Enterobacter cloacae strain TPL2（KJ470636.1）	99.79	Enterobacter
XB-14	Enterobacter cloacae strain TPL2（KJ470636.1）	99.79	Enterobacter
XB-15	Pseudomonas lalkuanensis strain PE08（CP043311.1）	99.72	Pseudomonas
XC-1	Paenibacillus dendritiformis strain PV3-16（MH472941.1）	99.65	Paenibacillus
XC-2	Bacillus subtilis strain kp6（MH200633.1）	99.65	Bacillus
XD-2	Bacillus megaterium strain S21（MT925631.1）	100	Bacillus
XD-3	Bacillus subtilis strain ge25（MW186208.1）	99.86	Bacillus
XD-6	Bacillus subtilis strain ge25（MW186208.1）	99.58	Bacillus
XD-9	Bacillus aryabhattai strain ZJJH-2（MT605509.1）	99.79	Bacillus
XD-10	Bacillus megaterium strain HX-2（MH930825.1）	99.65	Bacillus
XD-11	Fictibacillus barbaricus strain N7（KJ831620.1）	100	Fictibacillus
XD-13	Bacillus megaterium strain HX-2（MH930825.1）	99.72	Bacillus
XD-15	Bacillus subtilis strain T0-8（MN330082.1）	99.86	Bacillus
XD-17	Bacillus megaterium strain HX-2（MH930825.1）	99.79	Bacillus
XD-20	Bacillus aryabhattai strain fwz34（KF208484.1）	99.86	Bacillus
XD-23	Bacillus aryabhattai strain ZDX（MN473280.1）	99.86	Bacillus
XD-24	Bacillus subtilis strain MJP1（EU024822.1）	99.86	Bacillus
XD-25	Bacillus megaterium strain S1（MT453994.1）	100	Bacillus
XD-27	Priestia aryabhattai strain DT（MW673658.1）	99.93	Bacillus
XD-29	Bacillus pseudomycoides strain L59（KU179350.1）	99.79	Bacillus
XD-30	Bacillus megaterium strain HX-2（MH930825.1）	99.79	Bacillus
XD-34	Bacillus aryabhattai strain QH16-25（MT078622.1）	99.79	Bacillus
XD-36	Bacillus megaterium strain CS9（MG430216.1）	99.72	Bacillus
XI-1	Klebsiella grimontii strain RHBSTW-00853（CP056150.1）	99.79	Klebsiella
XI-3	Klebsiella grimontii strain RHBSTW-00854（CP056150.1）	99.64	Klebsiella
XI-7	Bacillus licheniformis strain HN-1（MH373532.1）	99.93	Bacillus