三种桑黄子实体脂溶性成分的GC-MS分析及抗氧化活性

doi:10.13560/j.cnki.biotech.bull.1985.2022-1275

摘要/Abstract

摘要：

比较分析不同桑黄子实体脂溶性成分差异，并初步探究其抗氧化活性。采用索氏提取法获得脂溶性成分并进行GC-MS分析，计算各化学成分的相对百分含量，测定其还原能力、对DPPH自由基、羟自由基的清除能力分析抗氧化活性。GC-MS结果表明共鉴定到176种化合物，JM1、JM2和JM3的脂溶性成分分别有68、75和71种化合物，对化合物进行归类分析发现主要含有烷类、酯类、芳香族类等物质，共有成分共8种，相对含量分别占JM1、JM2和JM3提取物的12.88%、9.85%、9.54%，分别为2,4-二甲基庚烷、乙苯、对二甲苯、苯乙烯、6-十二酮、棕榈酸甲酯、木蜡酸甲酯和四十四烷。3种子实体中JM1脂溶性物质具有更好的抗氧化活性。桑黄子实体脂溶性成分含有多种活性物质，具有一定的生物活性，可为深入研究其药理功效和资源开发提供参考。

关键词: 桑黄, 脂溶性成分, 气相色谱质谱联用仪

Abstract:

This work aims to study components and antioxidant activities of liposoluble components from different Sanghuangporus sp. fruiting bodies. Soxhlet extraction method was applied to extract the liposoluble components from different Sanghuangporus sp. fruiting bodies and their chemical compositions were identified by GC-MS. Its reducing ability was determined, and the antioxidant activity was evaluated by the reducing capacity, 1,1-diphenyl 1-diphenyl-2-picrylhydrazyl（DPPH）free radical scavenging ability and hydroxyl free radical scavenging ability. The GC-MS results suggested that there were 176 identified liposoluble components in total. Liposoluble components of JM1, JM2 and JM3 were 68, 75, 71 compounds, respectively. After classification and analysis, the compounds mainly contained alkane, ester, aromatic group and other substances. There are 8 common components, and their relative contents account for 12.88%, 9.85% and 9.54% of JM1, JM2 and JM3 extracts, respectively, which were 2,4-dimethylheptane, ethylbenzene, 1,4-xylene, styrene, 6-dodecanone, methyl hexadecanoate, methyl tetracosanoate and tetratetracontane. JM1 liposoluble components had the best antioxidant activities. The liposoluble components of three Sanghuangporus sp. fruiting bodies contained a variety of active substances and had various biological activities, which can provide a reference for the further study of their pharmacological efficacy and resource development.

Key words: Sanghuangporus sp., liposoluble components, GC-MS

罗阳兰, 曹乃馨, 杨玉梅, 黄丽玲, 阎勇, 黄世旅. 三种桑黄子实体脂溶性成分的GC-MS分析及抗氧化活性[J]. 生物技术通报, 2023, 39(6): 149-157.

LUO Yang-lan, CAO Nai-xin, YANG Yu-mei, HUANG Li-ling, YAN Yong, HUANG Shi-Lv. GC-MS Analysis and Antioxidant Activities of Liposoluble Components from Three Sanghuangporus Fruit Bodies[J]. Biotechnology Bulletin, 2023, 39(6): 149-157.

图/表 8

图1 菌株JM1形态特征 A：子实体；B：菌落；C：菌丝。下同

Fig. 1 Morphological characteristics of strain JM1 A：Fruiting body. B：Colony. C：Hyphae. The same below

图2 菌株JM2形态特征

Fig. 2 Morphological characteristics of strain JM2

图3 菌株JM3形态特征

Fig. 3 Morphological characteristics of strain JM3

图4 基于ITS序列的菌株系统发育树

Fig. 4 Phylogenetic tree of strain based on the ITS sequences

图5 脂溶性成分图谱 A、B和C分别为JM1、JM2和JM3的脂溶性成分图谱

Fig. 5 GC-MS profile of liposoluble components A, B and C are the GC-MS analysis of JM1, JM2 and JM3 liposoluble components respectively

图6 不同桑黄菌株子实体脂溶性提取物的铁离子还原能力

Fig. 6 Reducing capacities of liposoluble components from different Sanghuangporus sp. fruiting bodies

图7 不同桑黄菌株子实体脂溶性提取物对DPPH自由基的清除能力

Fig. 7 DPPH free radical scavenging abilities of liposoluble components from different Sanghuangporus sp. fruiting bodies

图8 不同桑黄菌株子实体脂溶性提取物对羟自由基的清除能力

Fig. 8 Hydroxyl radical scavenging abilities of liposoluble components from different Sanghuangporus sp. fruiting bodies

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