关键词: 白术, SCoT分子标记, 遗传多样性分析, DNA指纹图谱

Abstract:

【Objective】 Using SCoT molecular marker technology, the genetic diversity analysis and DNA fingerprinting were conducted on 17 germplasm resources of Atractylodes macrocephala, providing a theoretical basis for the identification, preservation, and breeding of new varieties of A. macrocephala germplasm. 【Method】 Thirteen core primers were selected from 92 SCoT primers for molecular labeling of A. macrocephala. Popgene1.32 software and NTSYS pc 2.10e software were used for diversity analysis and cluster analysis of A. macrocephala germplasm resources. A DNA molecular fingerprint map of A. macrocephala was constructed by combining the core primers of SCoT-9, SCoT-12, and SCoT-41. 【Result】 A total of 192 bands were obtained from 17 samples of A. macrocephala amplified by 13 SCoT core primers, including 165 polymorphic bands, with an average percentage of 85.94%. The genetic similarity coefficient（GS）and genetic distance（GD）ranged from 0.578 1 to 0.875 0 and 0.133 5 to 0.548 0, respectively. The observed number of alleles（Na）in the A. macrocephala germplasm was 1.859 4, the effective number of alleles（Ne）was 1.418 0, the Nei's genetic diversity index（He）was 0.256 3, and the Shannon information index（I）was 0.396 8. Cluster analysis showed that A. macrocephala was a cultivated variety, while the wild variety of Daweishan was separately clustered into another category. By the 17 constructed DNA fingerprint maps of A. macrocephala germplasm resources, the samples were distinguished and accurately identified. 【Conclusion】 The germplasm resources of A. macrocephala have relatively rich genetic diversity, but the genetic differences among cultivated A. macrocephala from different regions are low, indicating that geographical location is not a decisive factor in determining the distance of A. macrocephala kinship.

Key words: Atractylodes macrocephala, SCoT molecular markers, genetic diversity analysis, DNA fingerprinting