新鲜蔬果中食源性致病细菌研究进展

摘要/Abstract

摘要： 食用新鲜蔬果引起的食源性疾病快速增长，恶性事件时有发生。关注于国外与蔬果有关的致病细菌的研究进展，主要介绍了蔬果的微生物性质调查，分析了细菌总数、大肠菌群和致病细菌的分布规律；重要的致病细菌污染来源及影响污染率的原因；致病细菌在蔬果中的存活规律，并对重要因素进行排序；介绍了去除致病细菌通用方法和具有潜在应用价值的方式方法；致病细菌在蔬果中的附着方式，着重介绍了大肠杆菌O157：H7的3种附着方式。另外，还对相关的研究趋势提出了看法。

关键词: 新鲜蔬果, 食源性疾病, 致病细菌, 大肠杆菌O157：H7, 沙门氏菌

Abstract: The number of foodborne illness events caused by eating fresh vegetables and fruit grows rapidly, malignant events have occurred sometimes. This article focuses on the research progress of pathogens associated with vegetables and fruit abroad, vegetables and fruit were introduced microbial quality investigation, analyzed the distribution rule of total bacterial count, coliform group and pathogenic bacteria, the important pathogenic bacteria pollution sources and the influence factors of the pollution rate, the survival rule of pathogenic bacteria in vegetables and fruit, the important factors are sorted. About the methods of removing pathogenic bacteria, this paper introduces the general methods and the methods having potential application value；On the attachments of pathogenic bacteria to vegetables and fruit, this article introduces three adhesions of E. coli O157：H7. In addition, views of study trends are put forward.

Key words: Fresh vegetables and fruit, Foodborne diseases, Pathogenic bacteria, Escherichia coli, O157: H7 , Salmonella

刘一倩, 易欣欣. 新鲜蔬果中食源性致病细菌研究进展[J]. 生物技术通报, 2014, 0(4): 19-24.

Liu Yiqian, Yi Xinxin. Progress in Study of Foodborne Pathogens in Fresh Fruit and Vegetables[J]. Biotechnology Bulletin, 2014, 0(4): 19-24.

参考文献

[1] Acheson RM, Williams DR. Does consumption of fruit and vegetables protect against stroke?[J]. Lancet, 1983(1)：1191-1193.
[2] Anonymous. Diet, nutrition and the prevention of chronic diseases［R]. Report of a Joint FAO/WHO Expert Consultation. World Health Organization, 2003, Geneva(［Online]. Available at http：//www.fao.org/DOCREP/005/AC911E/AC911E00.HTM.
[3] Keskinen LA, Burke A, Annous BA. Efficacy of chlorine, acidic electrolyzed water and aqueous chlorine dioxide solutions to decontaminate Escherichia coli O157：H7 from lettuce leaves[J]. International Journal of Food Microbiology, 2009, 132：134-140.
[4] Seow J, ágoston R, Phua L, Yuk H. Microbiological quality of fresh vegetables and fruits sold in Singapore[J]. Food Control, 2012, 25：39-44.
[5] Allen KJ, Kovacevic J, Cancarevic A, et al. Microbiological survey of imported produce available at retail across Canada[J]. International Journal of Food Microbiology, 2013, 162：135-142.
[6] Oliveira M, Usall J, Vi?asa I, et al. Microbiological quality of fresh lettuce from organic and conventional production[J]. Food Microbiology, 2010, 27：679-684.
[7] Sivapalasingam S, Friedman CR, Cohen L, Tauxe RV. Fresh produce：a growing cause of out breaksof foodborne illness in the United States, 1973 through1997[J]. J Food Prot, 2004, 67：2342-2353.
[8] Arthur L, Jones S, Fabri M, Odumeru J. Microbial survey of selected ontariogrown fresh vegetables and fruits[J]. Journal of Food Protection, 2007, 70：2864-2867.
[9] Bohaychuk VM, Bradbury RW, Dimock R, et al. A microbiological survey of selected Alberta-grown fresh produce from farmers' markets in Alberta, Canada[J]. J Food Prot, 2009, 72：415-420.
[10] Westrell T, Ciampa N, Boelaert F, et al. Zoonotic infections in Eur-ope in 2007：a summary of the EFSA-ECDC annual report[J]. Euro Surveill, 2009, 14(3)：785-794.
[11] Awang Salleh N, Rusul G, Hassan Z, et al. Incidence of Salmonella spp. in raw vegetables in Selangor, Malaysia[J]. Food Control, 2003, 14：475-479.
[12] Islam M, Morgan J, Doyle MP, Jiang X. Fate of Escherichia coli O157：H7 in manure compost-amended soil and on carrots and on-ions grown in an environmentally controlled growth chamber[J]. J Food Prot, 2004, 67：574-578.
[13] Islam M, Morgan J, Doyle MP, et al. Fate of Salmonella enterica serovar Typhimurium on carrots and radishes grown in fields treated with contaminated manure composts or irrigation water[J]. Appl Environ Microbiol, 2004, 70：2497-2502.
[14] Orozco RL, Iturriaga MH, Tamplin ML, et al. Animal and environmental impact on the presence and distribution of Salmonella and Escherichia coli in hydroponic tomato greenhouses[J]. J Food Prot, 2008, 71：676-683.
[15] Talley JL, Wayadande AC, Wasala LP, et al. Association of Escherichia coli O157：H7 with filth flies(Muscidae and Calliphoridae)captured in leafy greens fields and experimental transmission of E. coli O157：H7 to spinach leaves by house flies(Diptera：Muscidae)[J]. J Food Prot, 2009, 72：1547-1552.
[16] Mitra R, Cuesta-Alonso E, Wayadande A, et al. Effect of route of introduction and host cultivar on the colonization, internalization, and movement of the human pathogen Escherichia coli O157：H7 in spinach[J]. J Food Prot, 2009, 72：1521-1530.
[17] Stine SW, Song I, Choi CY, Gerba CP. Application of microbial risk assessment to the development of standards for enteric pathogens in water used to irrigate fresh produce[J]. J Food Prot, 2005, 68：913-918.
[18] Sivapalasingam S, Friedman CR, Cohen L, Tauxe RV. Fresh produce：a growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997[J]. J Food Prot, 2004, 67：2342-2353.
[19] Kroupitski Y, Pinto R, Brandl MT, et al. Interactions of Salmonella enterica with lettuce leaves[J]. J Appl Microbiol, 2009, 106：1876-1885.
[20] Ukuku DO, Sapers GM. Effect of time before storage and storage temperature on survival of Salmonella inoculated on fresh-cut melons[J]. Food Microbiol, 2007, 24：288-295.
[21] Dreux N, Albagnac C, Carlin F, et al. Fate of Listeria spp. on parsley leaves grown in laboratory and field cultures[J]. J Appl Microbiol, 2007, 103：1821-827.
[22] Ibekwe AM, Watt PM, Shouse PJ, Grieve CM. Fate of Escherichia coli O157：H7 in irrigation water on soils and plants as validated by culture method and real-time PCR[J]. Can J Microbiol, 2004, 50：1007-1014.
[23] Ibekwe AM, Grieve CM, Papiernik SK, Yang CH. Persistence of Escherichia coli O157：H7 on the rhizosphere and phyllosphere of lettuce[J]. Lett Appl Microbiol, 2009, 49：784-790.
[24] Islam M, Morgan J, Doyle MP, Jiang X. Fate of Escherichia coli O157：H7 in manure compostamended soil and on carrots and onions grown in an environmentally controlled growth chamber[J]. J Food Prot, 2004, 67：574-578.
[25] Islam M, Doyle MP, Phatak SC, et al. Persistence of enterohemorrh-agic Escherichia coli O157：H7 in soil and on leaf lettuce and parsley grown in fields treated with contaminated manure composts or irrigation water[J]. J Food Prot, 2004, 67：1365-1370.
[26] Duffy B, Ravva S, Stanker L. Cantaloupe cultivar differences as opportunistic hosts for human pathogenic Escherichia coli O157：H7 and Salmonella[J]. Eur J Hortic Sci, 2008, 73：73-75.
[27] Arthurson V, Sessitsch A, J?derlund L. Persistence and spread of Salmonella enterica serovar Weltevreden in soil and on spinach plants[J]. FEMS Microbiol Lett, 2011, 314：67-74.
[28] Barak JD, Liang A, Narm KE. Differential attachment to and subsequent contamination of agricultural cropsby Salmonella enterica[J]. Appl Environ Microbiol, 2008, 74：5568-5570.
[29] Ragaert P, Devlieghere F, Debevere J. Role of microbiological and physio-logical spoilage mechanisms during storage of minimally processed vegetables[J]. Postharvest Biol Tech, 2007, 44：185-194.
[30] Delaquis P, Bach S, Dinu LD. Behavior of Escherichia coli O157：H7 in leafy vegetables[J]. J Food Prot, 2007, 70：1966-1974.
[31] Hutchison ML, Avery SM, Monaghan JM. The airborne distribution of zoonotic agents from livestock waste spreading and microbiologi-cal risk to fresh produce from contaminated irrigation sources[J]. J Appl Microbiol, 2008, 105：848-857.
[32] Li Y, Brackett RE, Chen J, Beuchat LR. Survival and growth of Escherichia coli O157：H7 inoculated onto cut lettuce before or after heating in chlorinated water followed by storage at 5 and 15uC[J]. J Food Prot, 2001, 64：305-309.
[33] Vandamma JP, Li D, Harris LJ, et al. Fate of Escherichia coli O157：H7, Listeria monocytogenes, and Salmonella on fresh-cut celery[J]. Food Microbiology, 2013, 34：151-157.
[34] Beuchat LR, Adler BB, Lang MM. Efficacy of chlorine and a peroxyacetic acid sanitizer in killing Listeria monocytogenes on iceberg and romaine lettuce using simulated commercial processing conditions[J]. J Food Prot, 2004, 67：1238-1242.
[35] Rodgers SL, Cash JN, Siddiq M, Ryser ET. A comparison of different chemical sanitizers for inactivating Escherichia coli O157：H7 and Listeria monocytogenes in solution and on apples, lettuce, strawberries, and cantaloupe[J]. Journal of Food Protection, 2004, 67：721-731.
[36] Stopforth JD, Mai T, Kottapalli B, Samadpour M. Effect of acidified sodium chlorite, chlorine, and acidic electrolyzed water on Escherichia coli O157：H7, Salmonella, and Listeria monocytogenes inoculated onto leafy greens[J]. Journal of Food Protection, 2008, 71：625-628.
[37] Viazis S, Akhtar M, Feirtag J, Diez-Gonzalez F. Reduction of Esch-erichia coli O157：H7 viability on leafy green vegetables by treat-ment with a bacteriophage mixture and trans-cinnamaldehyde[J]. Food Microbiology, 2011, 28：149-157.
[38] Puapermpoonsiri U, Spencer J, van derWalle C. A freeze-dried formulation of bacteriophage encapsulated in biodegradable microspheres[J]. Eur J Pharm Biopharm, 2009, 72：26-33.
[39] Jeter C, Matthysse AG. Characterization of the binding of diarrheagenic strains of E. coli to plant surfaces and the role of curli in the interaction of the bacteria with alfalfa sprouts[J]. Mol Plant Microbe Interact, 2005, 18：1235-1242.
[40] Xicohtencatl-Cortes J, Sanchez Chacon E, Saldana Z, et al. Interaction of Escherichia coli O157：H7 with leafy green produce[J]. J Food Prot, 2009, 72：1531-1537.
[41] Shaw RK, Berger CN, Feys B, et al. Enterohemorrhagic Escherichia coli exploits EspA filaments for attachment to salad leaves[J]. Appl Environ Microbiol, 2008, 74：2908-2914.
[42] Berger CN, Sodha SV, Shaw RK, et al. Fresh fruit and vegetables as vehicles for the transmission of human pathogens[J]. Environmental Microbiology, 2010, 12(9)：2385-2397.