一株淡色生赤壳菌的生防作用分析及系统发育树构建

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 184-189.

一株淡色生赤壳菌的生防作用分析及系统发育树构建

陈秀玲李景富张丽莉张俊峰王傲雪

(东北农业大学，哈尔滨 150030)

收稿日期:2013-11-13 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:陈秀玲，女，博士，助理研究员，研究方向：植物病害生物防治；E-mail：xiuling.chen@ymail.com
基金资助:
国家自然基金青年项目(31301780)，教育部科学技术研究重点项目(211043)，黑龙江省教育厅海外学人项目(1252HQ011)，教育厅新世纪优秀人才项目(1251-NECT-004)，东北农业大学博士启动金项目(2012RCB20)

Biocontrol Efficacy and Phylogenetic Tree Analysis of a New Bionectria ochroleuca Strain

Chen Xiuling Li Jingfu Zhang Lili Zhang Junfeng Wang Aoxue

(Northeast Agricultural University，Harbin 150030)

Received:2013-11-13 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 保护地栽培面积的增加使果蔬病害的防治难度加大，生物防治手段越来越受到青睐。采用平板对峙法、扫描电镜法、5.8S rDNA-ITS序列分析及系统发育树构建对一株从草炭土中分离的菌株进行研究。该菌对番茄灰霉病、番茄叶霉病、番茄绵疫病、番茄黄萎病、番茄枯萎病、杨树烂皮病、杨树枯萎病和黑穗醋栗叶斑病均有抑制作用。扫描电镜显示，该生防菌对番茄灰霉病具有明显抑制作用。5.8S rDNA-ITS序列分析及系统发育树构建表明该菌为淡色生赤壳菌，为果蔬病害的生物防治提供了新的选择。

关键词: 淡色生赤壳菌, 5.8S, rDNA-ITS, 系统发育树, 番茄灰霉病

Abstract: The fruit and vegetable disease controls are more difficult because the increase of protected cultivation area. Biological control means became more and more popular in disease control process. By dual culture on PDA plates, WY-1 inhibited the growth of Botrytis cinerea, Cladosporium fulvum, Phytophthora parasitica, Vertillium dahliae, Fusarium wilt, Valsa sordida Nit., Alternaria and Pseudopeziza ribis Kleb. Scanning electron microscopy revealed that WY-1 inhibited the Botrytis cinerea growth significantly. 5.8S rDNA-ITS sequence alignment and neighbor-joining phylogenetic tree reveal that WY-1 belongs to Bionectria ochroleuca, which may provide a new choice in disease control.

Key words: Bionectria ochroleuca 5.8S, RDNA-ITS, Phylogenetic tree, Botrytis Cinerea

陈秀玲, 李景富, 张丽莉, 张俊峰, 王傲雪. 一株淡色生赤壳菌的生防作用分析及系统发育树构建[J]. 生物技术通报, 2014, 0(5): 184-189.

Chen Xiuling, Li Jingfu, Zhang Lili, Zhang Junfeng, Wang Aoxue. Biocontrol Efficacy and Phylogenetic Tree Analysis of a New Bionectria ochroleuca Strain[J]. Biotechnology Bulletin, 2014, 0(5): 184-189.

参考文献

[1] Palmer CL, Horst RK, Langhans RW. Use of bicarbonates to inhibit in vitro colony growth of Botrytis cinerea[J]. Plant Disease, 1997, 81(12)：1432-1438.
[2] Knight SC, Anthony VM, Brady AM, et al. Rationale and perspectives on the development of fungicides[J]. Annual Reviews of Phytopathoogy, 1997, 35：349-372.
[3] Zhang CQ, Hu JL, Wei FL, et al. Evolution of resistance to different classes of fungicides in Botrytis cinerea from greenhouse vegetables in eastern China[J]. Phytoparasitica, 2009, 37：351-359.
[4] Sharma RR, Singh D, Singh R. Biological control of postharvest diseases of fruits and vegetables by microbial antagonists：A review[J]. Biological Control, 2009, 50：205-221.
[5] Janisiewicz WJ, Pimenta RS, Jurick WM. A novel method for selecting antagonists against postharvest fruit decays originating from latent infections[J]. Biological Control, 2011, 59(3)：384-389.
[6] Junaid JM, Dar NA, Bhat TA, et al. Commercial biocontrol agents and their mechanism of action in the management of plant patho-gens[J]. Int J Modern Plant& Anim Sci, 2013,1(2): 39-57.
[7] 张志华, 洪葵.核酸序列直接分析在真菌鉴定方面的应用[J].华南热带农业大学学报, 2006, 12(2)：39-42.
[8] Mark A, Ragan, Carolyn J, et al. Amolecular phylogeny of the marine red algae(Rhodophyta)based on the nuclear small-subunit rRNA gene[J]. Plant Biology, 1994, 91：7276-7280.
[9] Freshwarer DW, Fredericq S. A gene phylogeny of the red algae(Rhodophyta)based on plastid rbcL[J]. Plant Biology, 1994, 91：7281-7285.
[10] Gurgel CFD, Liao LM, Fredericq S, et al. Systematics of Gracilario-psis(Gracilariales, Rhodophyta)based on rbcL sequence analyses and morphological evidence[J]. Journal of Phycology, 2003, 39(1)：154-171.
[11] 李敏, 隋正红, 易恒, 等.龙须菜5.8S rRNA和ITS区的克隆与系统学分析[J].中国海洋大学学报, 2009, 39：77-83.
[12] 吴发红, 黄东益, 黄小龙, 等.几种真菌DNA提取方法的比较[J].中国农学通报, 2009, 25(8)：62-64.
[13] White TJ, Bruns TD, Lee SB, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, [M]//Imis MA. PCR Protocols：A guide to methods and applications. Academic Press, 1990：315-322.
[14] Larkin MA, Blackshields G, Brown NP, et al. Clustal W and Clustal X version 2.0[J]. Bioinformatics, 2007, 23：2947-2948.
[15] Tamura K, Peterson D, Peterson N, et al. MEGA5：molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 228(10)：2731-2739.
[16] 孙广宇, 彭友良, 李振歧, 等.核苷酸序列分析在真菌系统学研究中的应用[J].西北农林科技大学学报：自然科学版, 2003, 31(6)：187-192.
[17] 哈瑞根.食品微生物实验室手册[M].李卫华, 译.北京：中国轻工业出版社, 2004.
[18] 林晓民, 李振岐, 王少先.真菌rDNA的特点及在外生菌根菌鉴定中的应用[J].西北农业学报, 2005, 14(2)：120-125.