鲎素I对普通变形杆菌的作用研究

生物技术通报 ›› 2014, Vol. 0 ›› Issue (5): 190-196.

鲎素I对普通变形杆菌的作用研究

金元宝¹ 王英² 刘莉萍¹ 王倩¹ 金刚¹ 张丽君¹ 代建国¹

(1.深圳职业技术学院应用化学与生物技术学院，深圳 518055；2.广州城市职业学院食品系，广州 510405)

收稿日期:2013-10-23 出版日期:2014-05-23 发布日期:2014-05-24
作者简介:金元宝，男，硕士研究生，研究方向：抗菌肽的研究开发与利用；E-mail：2009jxncmeet@163.com
基金资助:
国家自然科学基金资助项目(30800793，31272474)，广东省自然科学基金项目(S2011020005160)，广东省高职院校珠江学者计划(2012)，广东省高校高层次人才计划(2011，2013)，广州市属高校科研计划 (2012A141)，深圳市基础研究重点项目(JC201105201191A)

Study the Effect of Tachyplesin I on Proteus vulgaris

Jin Yuanbao¹ Wang Ying² Liu Liping¹ Wang Qian¹ Jin Gang¹ Zhang Lijun¹ Dai Jianguo¹

(1. School of Applied Chemistry and Biotechnology，Shenzhen Polytechnic，Shenzhen 518055；2. Department of Food，Guangzhou City Polytechnic，Guangzhou 510405)

Received:2013-10-23 Published:2014-05-23 Online:2014-05-24

摘要/Abstract

摘要： 旨在研究鲎素Ⅰ对普通变形杆菌的抑杀作用机制。利用鲎素Ⅰ对普通变形杆菌的抑杀浓度、壁膜通透性和电荷性、分泌蛋白酶活力的影响及其在培养基中的稳定性进行研究。结果显示，鲎素Ⅰ对普通变形杆菌的MIC和MBC值分别为40-80和80 μg/mL，它也能增加普通变形杆菌的壁膜通透性及其所带的正电荷。在鲎素Ⅰ浓度≤40 μg/mL时，普通变形杆菌分泌的蛋白酶活力有所增加，而≥60 μg/mL时，则呈下降趋势。在普通变形杆菌培养环境中的鲎素Ⅰ含量会降低。结果证明，鲎素Ⅰ能对普通变形杆菌产生抑杀作用，其抑菌机制与破坏细菌壁膜的通透性有关。

关键词: 鲎素Ⅰ, 普通变形杆菌, 抑菌作用, 蛋白酶活

Abstract: It was to investigate the effect mechanism of tachyplesin I on Proteus vulgaris. The study on the inhibition concentration of tachyplesin I, the plasma membrane permeability and electric charge of Proteus vulgaris, the activity of proteases were secreted by Proteus vulgaris and the stability of tachyplesin I in the nutrient broth medium where cultured Proteus vulgaris. Results indicated that the MIC and MBC were 40-80 and 80 μg/mL, respectively. The membrane permeability and electric charge of Proteus vulgaris would be increased with the concentration of tachyplesin I increasing. The proteinase activity which secreted by Proteus vulgaris would be increased when the concentration of tachyplesin I less than 40 μg/mL, but it presented downward trend when more than 60 μg/mL. The concentration of tachyplesin I in the medium that trained the bacterial would reduce. Tachyplesin I could inhibit growth of the Proteus vulgaris, the effect mechanism related to damage the membrane permeability of them.

Key words: Tachyplesin I, Proteus vulgaris, Anti-bacterial, Nechanism, Proteinase activity

金元宝, 王英, 刘莉萍, 王倩, 金刚, 张丽君, 代建国,. 鲎素I对普通变形杆菌的作用研究[J]. 生物技术通报, 2014, 0(5): 190-196.

Jin Yuanbao, Wang Ying, Liu Liping, Wang Qian, Jin Gang, Zhang Lijun, Dai Jianguo,. Study the Effect of Tachyplesin I on Proteus vulgaris[J]. Biotechnology Bulletin, 2014, 0(5): 190-196.

参考文献

[1] Nakamura T, Furunaka H, Miyata T, et al. Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab(Tachypleus tridentatus)[J]. The Journal of Biological Chemistry, 1988, 263(32)：16709-16713.
[2] 代建国, 谢海伟, 金刚, 等.鲎素的抗菌靶点初探[J].生物化学与生物物理进展, 2008, 35(5)：563-569.
[3] 谢海伟, 杨贤松, 柯春林.鲎素抗菌肽的研究进展及应用前景[J].中国生物制品学杂志, 2013, 26(5)：740-744.
[4] Chen J, Xu XM, Underhill CB, et al. Tachyplesin activates the classic complement pathway to kill tumor cells[J]. Cancer Res, 2005, 65(11)：4614-4622.
[5] 柴晓杰, 王丕武, 徐雅维, 等.抗菌肽的研究进展及应用前景[J].中国饲料, 2005, 22(4)：15-17.
[6] 徐书显, 陈崇智.轻稀土硝酸盐对肠道细菌生长的影响[J].微生物学通报, 1988, 1：24-27.
[7] 刘慧明, 汪亚君, 丁航, 等.鲎抗菌肽对隐球菌、枯草杆菌、变形杆菌的抑菌作用[J].海南医学院学报, 2008, 14(1)：4-5.
[8] 曹海, 李永文, 雷雨, 等.大口鲇致病菌的分离鉴定及系统发育分析及相关特性的研究[J].微生物学报, 2007, 47(1)：1-6.
[9] 孙传波, 郭嘉, 陶蕊, 等.农杆菌介导玉米遗传转化体系的研究[J].中国农学通报, 2012, 28(36)：71-75.
[10] Giacometti A, Cirioni O, Barchiesi F, et al. In vitro susceptibility tests for cationic peptides：comparison of broth microdilution methods for bacteria that grow aerobically[J]. Antimicro Agents Chemo, 2000, 44(6)：1694-1696.
[11] 谢海伟, 代建国, 郭勇, 等.鲎素抗菌肽的分子结构稳定性及生物活性[J].华南理工大学学报：自然科学版, 2008, 36(4)：144-150.
[12] 鹿楠楠.尿毒清对红细胞膜和内皮细胞膜负电荷的影响[D].南宁：广西医科大学, 2009.
[13] 侯利霞, 翟培, 施用晖, 等.家蝇抗菌肽对细菌细胞表面特性影响及其作用机理的研究[J].微生物学通报, 2007, 34(3)：434-437.
[14] Lee HJ, Choi GJ, Cho KY. Correlation of lipid peroxidation in botrytis cinerea caused by dicarboximide fungicides with their fungicidal activity[J]. J Agric Food Chem, 1998, 46(2)：737-741.
[15] 艾启俊, 于庆华, 张红星, 等.鹿蹄草素对金黄色葡萄球菌的抑制作用及其机理研究[J].中国食品学报, 2007, 4(2)：33-37.
[16] 张相伟.饲用芽抱杆菌对鸡肠道发育和猫膜免疫调节作用的研究[D].武汉：华中农业大学, 2008.
[17] 谢海伟, 代建国, 金刚, 等.鲎素抗菌肽对仔猪腹泻致病菌细胞形态的影响[J].黑龙江畜牧兽医, 2008, 6：87-88.
[18] 洪军.鲎素能否诱导细菌产生抗药性及其相关机制的研究[D].广州：华南农业大学, 2011.
[19] 文加才, 秦永忠, 宋爱刚.阳离子抗菌肽的研究进展[J].国外医药抗生素分册, 2002, 23(6)：267-271.
[20] Perron GG, Zasloff M, Bell G. Experimental evolution of resistance to an antimicrobial peptide[J]. Proc R Soc B, 2006, 273：251-256.