毒素蛋白基因mazF在基因修饰系统中的应用进展

生物技术通报 ›› 2014, Vol. 0 ›› Issue (11): 48-54.

毒素蛋白基因mazF在基因修饰系统中的应用进展

石杨^1,2,董会娜²,张大伟²

1.天津大学药物科学与技术学院,天津 300072；2.中国科学院天津工业生物技术研究所,天津 300308

收稿日期:2014-03-27 出版日期:2014-11-07 发布日期:2014-11-07
作者简介:石杨,男,硕士研究生,研究方向：枯草芽孢杆菌代谢途径的改造
基金资助:
国家自然科学基金项目（31200036,31370089）,天津市科技支撑计划重点项目（12ZCZDSY12700,11ZCZDSY08500）

Application Progress of mazF Gene in Genetic Modification System

Shi Yang^1,2,Dong Huina²,Zhang Dawei²

1. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072；2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Science, Tianjin 300308

Received:2014-03-27 Published:2014-11-07 Online:2014-11-07

摘要/Abstract

摘要： 毒素-抗毒素系统（Toxin-antitoxin system,TA系统）存在于大部分细菌中。mazEF是大肠杆菌中一种毒素-抗毒素系统。毒素基因mazF编码的MazF毒素蛋白可以特异性地剪切自由mRNA的ACA序列,从而抑制蛋白合成、引起细胞生长停滞；近些年,许多学者利用mazF基因作为反向筛选标记对不同种微生物建立了无标记或无痕的基因修饰系统,并实现了不同菌株的基因组修饰。主要综述了大肠杆菌mazF基因作为反向筛选基因的应用原理及其在不同种类微生物的基因修饰系统中的应用进展,然后对mazF基因及其他毒素基因在基因修饰系统中的应用进行了展望。

关键词: mazF, 毒素蛋白基因, 反向筛选标记, 基因修饰方法

Abstract: Toxin-antitoxin system（TA）exists in most of the genetic material of bacteria. mazEF is a kind of toxin-antitoxin system in E. coli. MazF encoded by mazF is an mRNA interferase that specifically cleaves free mRNAs at ACA sequences, resulting in inhibited protein synthesis and cell growth arrest. Recently, some scholars have used mazF as a counter-selection marker in genetic modification systems, and achieved modifications of the genome in various strains. In this article, the research advances of E. coli mazF used as counter-selection marker in bacterial genetic modification were reviewed, followed by application of other toxin gene. Finally, perspective of the possible new pathways for developing new genetic modification methods were addressed.

Key words: mazF , Toxin gene , Counter-selection marker , Genetic modification method

石杨,董会娜,张大伟. 毒素蛋白基因mazF在基因修饰系统中的应用进展[J]. 生物技术通报, 2014, 0(11): 48-54.

Shi Yang,Dong Huina,Zhang Dawei. Application Progress of mazF Gene in Genetic Modification System[J]. Biotechnology Bulletin, 2014, 0(11): 48-54.

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