毒死蜱的环境生物学效应分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.08.033

生物技术通报 ›› 2015, Vol. 31 ›› Issue (8): 225-230.doi: 10.13560/j.cnki.biotech.bull.1985.2015.08.033

• 研究报告 • 上一篇

毒死蜱的环境生物学效应分析

余凯敏, 冯为民, 李国超, 张家禹, 刘丽丽, 闫艳春

中国农业科学院研究生院,北京 100081

收稿日期:2014-12-09 出版日期:2015-08-21 发布日期:2015-08-22
作者简介:余凯敏,男,硕士研究生,研究方向：微生物分子生物学与基因工程;E-mail：kmyu1989@gmail.com
基金资助:
国家自然科学基金项目（31170119）,中国农业科学院基础研究基金项目（0042014006,0042012003,0042011006）

An Analysis of Environmental and Biological Effects of Chlorpyrifos

Yu Kaimin, Feng Weimin, Li Guochao, Zhang Jiayu, Liu Lili, Yan Yanchun

Graduate School,Chinese Academy of Agricultural Sciences,Beijing 100081

Received:2014-12-09 Published:2015-08-21 Online:2015-08-22

摘要/Abstract

摘要： 高毒有机磷农药禁用以后,毒死蜱作为其替代品逐渐开始大规模应用。毒死蜱在水中降解缓慢,因此在水中的残留会对水生生物及其他生物造成潜在危害。为探究低浓度毒死蜱的内分泌干扰效应,使用流式细胞仪分析了其对人子宫内膜癌细胞HEC-1B生长周期的影响。为探究高浓度毒死蜱的生物毒性,将斑马鱼胚胎暴露在不同浓度的毒死蜱（0、1.0、2.0、3.0和4.0 ppm）中60 h,发现毒死蜱能导致斑马鱼胚胎的死亡和严重畸形,并且胚胎存活率与处理浓度呈负相关,畸形率与处理浓度呈正相关。最后,检测了毒死蜱处理后斑马鱼胚胎中5种神经系统发育相关基因的表达情况。结果表明,低浓度毒死蜱具有内分泌干扰效应,高浓度毒死蜱会影响斑马鱼神经系统的正常发育。

关键词: 毒死蜱, 环境生物学效应, 斑马鱼胚胎, 神经发育毒性, 内分泌干扰效应

Abstract: With the prohibition of high-toxicity organophosphorous pesticides, the chlorpyrifos as their substitute has been used in a large-scale. The residue of chlorpyrifos, degrading slowly in the water, may cause the potential damages to aquatic organisms and others. To investigate the endocrine disrupting effects of low-concentration chlorpyrifos, flow cytometry was used to analyze the growth cycle of endometrial cancer cell HEC-1B of human. Moreover, zebrafish embryos were selected for chlorpyrifos exposing treatment 60 h at various concentrations of 0, 1.0, 2.0, 3.0 and 4.0 ppm. It was found that the chlorpyrifos caused the zebrafish embryos to death and significant embryonic malformation, the survival rate of the embryos was inversely proportional to chlorpyrifos’ concentration, and the malformation rate was proportional to chlorpyrifos’ concentration. Further, the mRNA expression levels of 5 neurodevelopment maker genes in the chlorpyrifos-treated zebrafish embryos were detected. These results showed that chlorpyrifos of low-concentration acted as endocrine disruptor, and chlorpyrifos of high-concentration affected the normal development of nerve system.

Key words: chlorpyrifos, environmental and biological effects, zebrafish embryo, developmental neurotoxicity, endocrine disrupting effect

余凯敏, 冯为民, 李国超, 张家禹, 刘丽丽, 闫艳春. 毒死蜱的环境生物学效应分析[J]. 生物技术通报, 2015, 31(8): 225-230.

Yu Kaimin, Feng Weimin, Li Guochao, Zhang Jiayu, Liu Lili, Yan Yanchun. An Analysis of Environmental and Biological Effects of Chlorpyrifos[J]. Biotechnology Bulletin, 2015, 31(8): 225-230.

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毒死蜱的环境生物学效应分析

An Analysis of Environmental and Biological Effects of Chlorpyrifos

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