土壤中木霉的分离及其对毒死蜱降解特性研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.030

生物技术通报 ›› 2016, Vol. 32 ›› Issue (6): 205-210.doi: 10.13560/j.cnki.biotech.bull.1985.2016.06.030

土壤中木霉的分离及其对毒死蜱降解特性研究

张广志, 张新建, 李红梅, 郭凯, 杨合同

山东省科学院生态研究所山东省应用微生物重点实验室，济南 250014

收稿日期:2015-09-30 出版日期:2016-06-27 发布日期:2016-06-28
作者简介:张广志，男，助理研究员，研究方向：农业微生物资源挖掘及应用；E-mail：zhanggzh@sdas.org
基金资助:
山东省科技发展计划(2014GSF121028，2013GNC11019)，国家科技基础性工作专项(2014FY120900)

Isolation and Characterization of the Chlorpyrifos-degrading Trichoderma Strains from the Vegetable Soil in Greenhouse

ZHANG Guang-zhi, ZHANG Xin-jian, LI Hong-mei, GUO Kai, YANG He-tong

Institute of Ecology，Shandong Academy of Sciences，Shandong Provincial Key Lab for Applied Microbiology，Jinan 250014

Received:2015-09-30 Published:2016-06-27 Online:2016-06-28

摘要/Abstract

摘要： 为挖掘保护地土壤中的能降解毒死蜱的木霉资源，从长期污染的土壤中共分离6个不同木霉菌株，通过形态学特征和ITS rDNA序列对各菌株进行鉴定，6个菌株分别为哈茨木霉(Trichoderma harzianum)、绿色木霉(T. viride)、康宁木霉(T. koningii)、拟康宁木霉(T. koningiopsis)、长枝木霉(T. longibrachiatum)和短密木霉(T. brevicompactum)。选择降解活性最高的长枝木霉TC5菌株，测定各种条件下对毒死蜱的降解特性。结果表明，木霉TC5在中性或偏碱性条件下对毒死蜱具有更好的降解活性；添加碳源或提高木霉的接种浓度，能提高对毒死蜱的降解活性；在50-300 mg/L范围内，随毒死蜱浓度升高，木霉的降解活性也明显提高。盆栽试验中木霉TC5对毒死蜱保持较强的降解活性，但在自然土壤中降解活性显著低于在灭菌土壤中。分离的木霉菌株在土壤农药污染修复方面具有应用开发潜力。

关键词: 木霉, 毒死蜱, 生物降解

Abstract: To explore and protect the Trichoderma resources that can degrade chlorpyrifos，six different types of Trichoderma strains with high activity of degrading chlorpyrifos were screened from the long-term organophosphorus pesticide contaminated soil. Analyses of morphological characteristics combined with internal transcribed spacer(ITS)rDNA sequences were used to identify them as T. harzianum, T. viride, T. koningii, T. koningiopsis, T. longibrachiatum，and T. brevicompactum，respectively. T. longibrachiatum TC5 with the highest degradation activity was selected to investigate the characterization of degrading chlorpyrifos under the culture condition including extra carbon source，pesticide concentration，inoculum density and pH. Under neutral or alkaline conditions，the strains TC5 had solid degradation activity to chlorpyrifos. Either adding carbon or increasing the inoculum concentration promoted the degradation rate. Degradation activity gradually increased with the increasing of chlorpyrifos concentration in the range of 50-300 mg/L. In the pot experiment，Trichoderma TC5 remained the high degradation activity to chlorpyrifos，but the degradation activity in the natural soil was significantly lower than in sterilized soil. The Trichoderma strains have great potential application in remedying the pesticide-contaminated soil.

Key words: Trichoderma, chlorpyrifos, biodegradation

张广志, 张新建, 李红梅, 郭凯, 杨合同. 土壤中木霉的分离及其对毒死蜱降解特性研究[J]. 生物技术通报, 2016, 32(6): 205-210.

ZHANG Guang-zhi, ZHANG Xin-jian, LI Hong-mei, GUO Kai, YANG He-tong. Isolation and Characterization of the Chlorpyrifos-degrading Trichoderma Strains from the Vegetable Soil in Greenhouse[J]. Biotechnology Bulletin, 2016, 32(6): 205-210.

参考文献

[1]Anwar S, Liaquat F, Khan QM, et al. Biodegradation of chlorpyrifos and its hydrolysis product 3, 5, 6-trichloro-2-pyridinol by Bacillus pumilus strain C2A1[J]. Journal of Hazardous Materials, 2009, 168：400-405.
[2]Yu L, Fang H, Wang X, et al. Characterization of a fungal strain capable of degrading chlorpyrifos and its use in detoxification of the insecticide on vegetables[J]. Biodegradation, 2006, 17：487-494.
[3]Yang L, Zhao Y, Zhang B, et al. Isoation and characterization of a chlorpyrifos and 3, 5, 6-trichloro-2-pyridinol degrading bacterium[J]. FEMS Microbiology Letters, 2005, 251：67-73.
[4]武春媛, 陈楠, 李勤奋, 等. 毒死蜱降解菌及其降解机理研究进展[J]. 热带作物学报, 2011, 32(10)：1989-1994.
[5]Ezzi MI, Lynch JM. Biodegradation of cyanide by Trichoderma spp. and Fusarium spp.[J]. Enzyme Microb Technol, 2005, 36：849-854.
[6]Tripathi P, Singh PC, Mishra A, et al. Trichoderma：a potential bioremediator for environmental clean up[J]. Clean Techn Environ Policy, 2013, 15：541-550.
[7]Xu G, Li Y, Zheng W, et al. Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.[J]. Biotechnol Lett, 2007, 29：1469-1473.
[8]Venkateswarlu K, Siddarame Gowda TK, Sethunathan N. Persistence and biodegradation of carbofuran in flooded soils[J]. Journal of Agricultural and Food Chemistry, 1977, 25(3)：533-536.
[9]Zhang C, Druzhinina IS, Tong X, et al. Trichoderma biodiversity in China：evidence for a North to South distribution of species in East Asia[J]. FEMS Microbiol Letts, 2005, 25(3)：251-257.
[10]Lan W, Cong J, Jiang H, et al. Expression and characterization of carboxylesterase E4 gene from peachpotato aphid(Myzus persicae)for degradation of carbaryl and malathion[J]. Biotechnol Lett, 2005, 27：1141-1146.
[11]石利利, 林玉锁, 徐亦钢, 等. 高尔夫球场土壤和水中毒死蜱农药残留的测定[J]. 农业生态环境, 2000, 16(3)：35-38.
[12] Singh BK, Walker A, Morgan JAW, et al. Biodegradation of chlor-pyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils[J]. Appl Environ Microbiol, 2004, 70：4855-4863.
[13]张广志, 杨合同, 张新建, 等. 毒死蜱降解木霉菌对几种重要植物病原真菌的生防活性[J]. 菌物学报, 2014, 33(6)：1292-1301.

[1]	罗宁, 焦阳, 茆振川, 李惠霞, 谢丙炎. 木霉菌对根结线虫和孢囊线虫防治机理研究进展[J]. 生物技术通报, 2023, 39(2): 35-50.
[2]	赵忠娟, 杨凯, 扈进冬, 魏艳丽, 李玲, 徐维生, 李纪顺. 盐胁迫条件下哈茨木霉ST02对椒样薄荷生长及根区土壤理化性质的影响[J]. 生物技术通报, 2022, 38(7): 224-235.
[3]	张豪, 李哲, 郭凯, 黄艳华, 郝永任. 绿色木霉Tv-1511组蛋白乙酰化酶编码基因TvGCN5的功能分析[J]. 生物技术通报, 2022, 38(5): 136-148.
[4]	祝静, 于存. 长枝木霉菌肥对玉米生长、土壤肥力和根际微生物的影响[J]. 生物技术通报, 2022, 38(4): 230-241.
[5]	付雅丽, 彭万里, 林双君, 邓子新, 梁如冰. 香茅醇假单胞菌SJTE-3的短链脱氢酶SDR-X1的克隆及酶性质测定[J]. 生物技术通报, 2022, 38(3): 121-129.
[6]	马青云, 江旭, 李情情, 宋金龙, 周义清, 阮志勇. 烟嘧磺隆降解菌Chryseobacterium sp. LAM-M5的分离、鉴定及其降解机理研究[J]. 生物技术通报, 2022, 38(2): 113-122.
[7]	牛鸿宇, 舒倩, 杨海君, 颜智勇, 谭菊. 一株十二烷基硫酸钠高效降解菌的分离鉴定、降解特性及代谢途径研究[J]. 生物技术通报, 2022, 38(12): 287-299.
[8]	武玉环, 彭焕, 葛逢勇, 彭德良, 刘大群, 李亚宁. 5株生防真菌对孢囊线虫的杀线活性测定[J]. 生物技术通报, 2022, 38(11): 220-226.
[9]	肖小双, 安雪姣, 叶晗媛, 王林平, 钟斌, 张庆华. 废水中硫氰酸盐的微生物降解研究进展[J]. 生物技术通报, 2021, 37(2): 224-235.
[10]	张雅静, 宋美燕, 张怡静, 房庆, 杨俊, 彭德良, 黄文坤, 彭焕, 朱英波, 孔令安. 兼防黄瓜根腐病和根结线虫病的淡紫拟青霉和哈茨木霉的筛选[J]. 生物技术通报, 2021, 37(2): 40-50.
[11]	王豪, 唐禄鑫, 马鸿飞, 钱坤, 司静, 崔宝凯. 东方栓孔菌漆酶的固定化及其对不同类型染料的脱色作用[J]. 生物技术通报, 2021, 37(11): 142-157.
[12]	陶治东, 何艳慧, 邓子禾, 孙琳琳, 武占省. 香菇菌渣高效纤维素降解菌的筛选及产酶优化[J]. 生物技术通报, 2021, 37(11): 158-165.
[13]	吴敏, 唐洁, 胡琼, 雷丹, 张庆. 表面活性剂对琼式不动杆菌LH-1-1降解溴氰菊酯的影响[J]. 生物技术通报, 2021, 37(1): 215-222.
[14]	岳丽晓, 李登云, 张晶晶, 仝雷. 一株敌草隆降解菌的分离及其应用潜能探索[J]. 生物技术通报, 2020, 36(6): 110-119.
[15]	陈锐, 瞿佳, 孙晓宇, 邓媛, 门欣, 赵玲侠, 沈卫荣. 氯氰菊酯降解菌草酸青霉SSCL-5分离鉴定及降解特性[J]. 生物技术通报, 2020, 36(6): 120-127.

土壤中木霉的分离及其对毒死蜱降解特性研究

Isolation and Characterization of the Chlorpyrifos-degrading Trichoderma Strains from the Vegetable Soil in Greenhouse

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价