关键词: 高通量染色质构象捕获技术, 染色质交互, 基因组组装, scaffolds锚位

Abstract: With the rapid development of sequencing technology, DNA sequencing is more efficiently and economically and in greater depth than ever before. How to locate the scaffolds into the chromosome becomes the key of getting high-quality genome. High-throughput chromatin conformation capture technique provides a new opportunity for scaffolds anchoring. Compared with traditional method, an assembly method based on chromosome interaction information is simple, low cost experimentation and saving time, and more application is expected in other relative complex polyploidy species. However, because of the limitation of the related technology, the genome assembly based on chromatin interaction still use second-generation technology, there are still many problems to be solved, for example, the resolution and background noise. It is expected to further improvement and enhancement. We still need to make effort to improve and optimize this method.

Key words: high-throughput chromosome conformation capture, chromatin interaction, genome assembly, scaffolds anchoring