水稻白叶枯病菌第二信使c-di-GMP代谢酶基因的预测和分析

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.016

生物技术通报 ›› 2015, Vol. 31 ›› Issue (11): 131-138.doi: 10.13560/j.cnki.biotech.bull.1985.2015.11.016

水稻白叶枯病菌第二信使c-di-GMP代谢酶基因的预测和分析

薛丁榕, 田芳, 李海云, 杨凤环, 陈华民, 何晨阳

中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室，北京 100193

收稿日期:2015-05-19 出版日期:2015-11-26 发布日期:2015-11-26
作者简介:薛丁榕，女，硕士，研究方向: 分子植物病理学；E-mail: xdingrong@163.com
基金资助:
国家自然科学基金项目(31370160)，北京市自然科学基金项目(5142017)

Prediction and Analysis of Genes Encoding c-di-GMP-Metabolizing Enzymes in Xanthomonas oryzae pv. oryzae

Xue Dingrong, Tian Fang, Li Haiyun, Yang Fenghuan, Chen Huamin, He Chenyang

State Key Laboratory for Biology of Plant Diseases and Insect Pests，Institute of Plant Protection，Chinese Academy of Agricultural Sciences，Beijing 100193

Received:2015-05-19 Published:2015-11-26 Online:2015-11-26

摘要/Abstract

摘要： 旨在从水稻白叶枯病菌(Xanthomonas oryzae pv. oryzae，Xoo)中鉴定出含有GGDEF结构域的鸟苷酸环化酶(DGC)和含有EAL或HD-GYP结构域的磷酸二酯酶(PDE)编码基因。预测分析了Xoo已测序菌株中的GGDEF、EAL和HD-GYP结构域蛋白的种类、数量及其序列特征，鉴别了基序保守的DGC或PDE、基序退化的信号受体以及信号输入结构域，验证了部分不同类型结构域蛋白作为DGC/PDE、信号受体以及在细菌毒性调控等方面的功能。

关键词: 水稻白叶枯病菌, c-di-GMP信号途径, 鸟苷酸环化酶, 磷酸二酯酶

Abstract: Cyclic di-GMP, a ubiquitous bacterial second messenger that regulates diverse cellular processes including virulence, is synthesized by diguanylate cyclase(DGC)with the GGDEF domain and degraded by phosphodiesterase(PDE)with the EAL or HD-GYP domain, respectively. To identify genes encoding DGCs and PDEs in Xanthomonas oryzae pv. oryzae, prediction and analysis of the GGDEF, EAL and HD-GYP domain proteins in three genome-sequenced strains PXO99^A, MAFF311018 and KACC10331 was comparatively performed in this study. Twenty-five to twenty-six GGDEF, EAL and HD-GYP domain protein genes were identified from each of three strains. Eighteen motif-conserved proteins were predicted to be the active DGCs or PDEs to metabolize c-di-GMP, whereas the remaining with the degenerate motifs might be the c-di-GMP receptors. Moreover, the majority of proteins were demonstrated to contain the input sensor domains to perceive the environmental stimulation and thereby modulate the activity of downstream output domains. The part of proteins was experimentally validated for their functions in the c-di-GMP signaling and regulation of virulence in X. oryzae pv. oryzae.

Key words: Xanthomonas oryzae pv. oryzae, c-di-GMP signaling, diguanylate cyclase, phosphodiesterase

薛丁榕, 田芳, 李海云, 杨凤环, 陈华民, 何晨阳. 水稻白叶枯病菌第二信使c-di-GMP代谢酶基因的预测和分析[J]. 生物技术通报, 2015, 31(11): 131-138.

Xue Dingrong, Tian Fang, Li Haiyun, Yang Fenghuan, Chen Huamin, He Chenyang. Prediction and Analysis of Genes Encoding c-di-GMP-Metabolizing Enzymes in Xanthomonas oryzae pv. oryzae[J]. Biotechnology Bulletin, 2015, 31(11): 131-138.

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水稻白叶枯病菌第二信使c-di-GMP代谢酶基因的预测和分析

Prediction and Analysis of Genes Encoding c-di-GMP-Metabolizing Enzymes in Xanthomonas oryzae pv. oryzae

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