水稻白叶枯病菌T3SS基因表达及其调控网络

doi:10.13560/j.cnki.biotech.bull.1985.2017-1011

摘要/Abstract

摘要： 植物病原细菌III型分泌系统（T3SS）在其毒性表达及与寄主互作中具有重要的功能。水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae,Xoo）hrp基因簇编码了T3SS装置,将毒性效应子蛋白分泌到水稻细胞内,抑制和破坏寄主免疫反应,或诱导感病基因表达,以达到成功侵染的目的。hrp基因表达受到严格调控,在模拟水稻内环境的贫瘠营养培养基中被诱导表达;hrp和效应子基因均受调控蛋白HrpG和HrpX的调控。此外,hrp基因还受到其它毒性调控网络重要因子的调控,包括双组分调控因子、转录调控因子、DNA/RNA结合蛋白、糖代谢和c-di-GMP信号因子。结合本实验室的研究结果,综述了Xoo T3SS表达调控及其致病机理的研究进展,以期为水稻细菌病害发生机理的解析及其有效防控措施提供一些新见解、思路和途径。

关键词: 水稻白叶枯病菌, III型分泌系统, 基因表达, 调控网络

Abstract: The type III secretion system（T3SS）plays a crucial role in the virulence of plant pathogenic bacteria and their interactions with hosts. The hrp genes of Xanthomonas oryzae pv. oryzae（Xoo）are involved in the construction of T3SS,through which dozens of virulence-related proteins,called toxic effectors,are directly secreted into rice cells to suppress and disturb plant immune systems,or to induce plant-susceptible genes,thus successfully infecting plants. The expression of hrp genes is strictly regulated and induced only in certain nutrient-poor media of simulating rice inner environment. Two proteins,HrpG and HrpX,are known as key regulators for hrp and effector gene expression. Besides,many regulators are also involved in this regulation process,including two-component system（TCS）,transcriptional regulators,DNA/RNA binding proteins,carbohydrate metabolisms and c-di-GMP related proteins. In this review,we summarized the regulatory network and virulence mechanisms of T3SS in Xoo,in order to provide novel insights into the effective and sustainable disease control of bacterial blight of rice.

Key words: Xanthomonas oryzae pv. oryzae, type III secretion system, gene expression, regulatory network

范素素, 田芳, 何晨阳. 水稻白叶枯病菌T3SS基因表达及其调控网络[J]. 生物技术通报, 2018, 34(2): 38-44.

FAN Su-su, TIAN Fang, HE Chen-yang. Regulation and Expression of Genes Encoding the Type III Secretion System in Xanthomonas oryzae pv. oryzae[J]. Biotechnology Bulletin, 2018, 34(2): 38-44.

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