感染时间对MDCK细胞中H1N1甲型流感病毒扩增过程的影响

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.032

生物技术通报 ›› 2015, Vol. 31 ›› Issue (11): 243-250.doi: 10.13560/j.cnki.biotech.bull.1985.2015.11.032

感染时间对MDCK细胞中H1N1甲型流感病毒扩增过程的影响

黄锭¹, 刘旭平¹, 范里¹, 赵亮¹, 谭文松¹, 陈则²

1.华东理工大学生物反应器工程国家重点实验室，上海 200237；2.上海生物制品研究所有限责任公司分子病毒研究室，上海 200052

收稿日期:2015-07-02 出版日期:2015-11-26 发布日期:2015-11-26
作者简介:黄锭，男，博士，研究方向: 动物细胞与组织工程；E-mail: huang-ding@139.com
基金资助:
国家自然科学基金项目(21206040，21406066)，国家“863”计划(2012AA02A303)，国家重大专项(2013ZX10004003-003-003)，中央高校基本科研业务费(WF1214035)

Effects of TOI on H1N1 Virus Propagation in MDCK Cell Culture

Huang Ding¹, Liu Xuping¹, Fan Li¹, Zhao Liang¹, Tan Wensong¹, Chen Ze²

1. State Key Lab of Bioreactor Engineering，East China University of Science and Technology，Shanghai 200237；2. Laboratory of Molecular Virology，Shanghai Institute of Biological Products Co.，Ltd.，Shanghai 200052

Received:2015-07-02 Published:2015-11-26 Online:2015-11-26

摘要/Abstract

摘要： 为了研究感染时间TOI对MDCK细胞中H1N1甲型流感病毒扩增过程的影响，以期合理优化以MDCK细胞培养技术为基础的H1N1流感病毒生产工艺，从而提高其生产效率。在不同TOI条件下以H1N1流感病毒感染MDCK细胞，考察TOI对MDCK细胞生长与代谢动力学以及H1N1流感病毒扩增过程的影响。结果表明，当TOI为72 h时，H1N1流感病毒产量最高。然而单位细胞病毒产率却随TOI增大而呈现下降趋势，进一步剖析TOI与感染时细胞密度CCI对单位细胞病毒产率的影响可知，该现象是由细胞状态而非高细胞密度所致。上述研究揭示了由TOI不同导致的细胞状态差异对MDCK细胞中H1N1流感病毒生产效率的重要性。

关键词: 感染时间, 流感病毒, MDCK细胞

Abstract: In order to improve the production efficiency of influenza vaccine in processes based on MDCK cell culture, the effects of TOI on influenza A(H1N1)virus propagation in MDCK cells were investigated. By infecting MDCK cells with H1N1 influenza virus at different TOI, the characteristics of MDCK growth and metabolism and influenza A(H1N1)virus were studied. The results showed that maximum influenza A(H1N1)virus yield reached when TOI was 72 h. However, the cell specific virus yield was reduced as TOI increased. To find out the reason behind this phenomenon, further analysis of TOI and CCI effects on cell specific virus yield were studied. Results showed that, the phenomenon was caused by cell status rather than high cell density. The study reveals the importance of cell status difference due to TOI, showed that influenza A(H1N1)virus production efficiency in MDCK cells could be improved by cell state adjusting.

Key words: TOI, influenza virus, MDCK cells

黄锭, 刘旭平, 范里, 赵亮, 谭文松, 陈则. 感染时间对MDCK细胞中H1N1甲型流感病毒扩增过程的影响[J]. 生物技术通报, 2015, 31(11): 243-250.

Huang Ding, Liu Xuping, Fan Li, Zhao Liang, Tan Wensong, Chen Ze. Effects of TOI on H1N1 Virus Propagation in MDCK Cell Culture[J]. Biotechnology Bulletin, 2015, 31(11): 243-250.

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感染时间对MDCK细胞中H1N1甲型流感病毒扩增过程的影响

Effects of TOI on H1N1 Virus Propagation in MDCK Cell Culture

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