γ-谷氨酰转肽酶补料法合成L-茶氨酸工艺研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.02.022

生物技术通报 ›› 2016, Vol. 32 ›› Issue (2): 165-171.doi: 10.13560/j.cnki.biotech.bull.1985.2016.02.022

γ-谷氨酰转肽酶补料法合成L-茶氨酸工艺研究

傅嘉懿¹, 陈晟², 吴敬¹

1.江南大学食品科学与技术国家重点实验室，无锡 214122；2.江南大学生物工程学院工业生物技术教育部重点实验室，无锡 214122

收稿日期:2015-03-12 出版日期:2016-02-24 发布日期:2016-02-25
作者简介:傅嘉懿，女，硕士研究生，研究方向：工业酶技术；E-mail：rorjil@hotmail.com

A Research of L-theanine Synthesis with Two Feeding Methods of L-glutamine Using γ-glutamyltranspeptidase

FU Jia-yi¹, CHEN Sheng², WU Jing¹

(1.State Key Laboratory of Food Science and Technology，Jiangnan University，Wuxi 214122；2.Key Laboratory of Industrial Biotechnology of Ministry of Education，School of Biotechnology，Jiangnan University，Wuxi 214122)

Received:2015-03-12 Published:2016-02-24 Online:2016-02-25

摘要/Abstract

摘要： 对γ-谷氨酰转肽酶酶法制备L-茶氨酸的工艺进行优化。通过恒速补料的策略，以200 mmol/L L-谷氨酰胺和2 mol/L乙胺盐酸盐作为初始底物，37℃、pH10.0条件下反应，每2 h补加100 mmol L-谷氨酰胺，反应14 h，最终L-谷氨酰胺底物总浓度为900 mmol/L，L-茶氨酸的生成量达到573.2 mmol/L，转化率达到63.7%，生产强度为40.9 mmol/(h·L)。采用变速流加的工艺，以同样的初始条件进行反应，每2 h补加L-谷氨酰胺至初始浓度200 mmol/L，反应15 h，最终L-谷氨酰胺总浓度为600 mmol/L，L-茶氨酸的生成量达到445.8 mmol/L，转化率为74.3%，生产强度为29.7 mmol/(h·L)。

关键词: γ-谷氨酰转肽酶, L-茶氨酸, 恒速补料, 变速补料

Abstract: The optimization of L-theanine production by γ-GGT(gamma-glutamyltranspeptidase)was investigated.The constant speed feeding strategy was performed using 200 mmol/L L-glutamine and 2 mol/L ethylamine hydrochloride as the initial substrates，under the condition of 37℃，pH10.0，100 mmol/L L-glutamine was added every 2 hours and the reaction sustained for 14 hours.Eventually the total concentration of L-glutamine and L-theanine were 900 mmol/L and 573.2 mmol/L respectively.The conversion rate of L-theanine reached 63.7%，and the production intensity was 40.9 mmol/(h·L).The variable speed feeding process conducted for 15 hours with the same initial conditions，and the adding L-glutamine to the initial concentration of 200 mmol/L in every 2 hours.After 15 hours，600 mmol/L of L-glutamine was converted to L-theanine with a final concentration of 445.8 mmol/L and the conversion rate and production intensity reached 74.3% and 29.7 mmol/(h·L).

Key words: γ-glutamyltranspeptidase, L-theanine, constant speed feeding, constant speed feeding

傅嘉懿, 陈晟, 吴敬. γ-谷氨酰转肽酶补料法合成L-茶氨酸工艺研究[J]. 生物技术通报, 2016, 32(2): 165-171.

FU Jia-yi, CHEN Sheng, WU Jing. A Research of L-theanine Synthesis with Two Feeding Methods of L-glutamine Using γ-glutamyltranspeptidase[J]. Biotechnology Bulletin, 2016, 32(2): 165-171.

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γ-谷氨酰转肽酶补料法合成L-茶氨酸工艺研究

A Research of L-theanine Synthesis with Two Feeding Methods of L-glutamine Using γ-glutamyltranspeptidase

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