microRNA-483和microRNA-486在克隆和转fat-1基因牛组织中的表达

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.017

生物技术通报 ›› 2016, Vol. 32 ›› Issue (3): 105-108.doi: 10.13560/j.cnki.biotech.bull.1985.2016.03.017

microRNA-483和microRNA-486在克隆和转fat-1基因牛组织中的表达

吕洋¹,王煜^1,2,孙佳佳¹,弓春玲¹,李光鹏¹

1. 内蒙古大学实验动物研究中心, 呼和浩特 010070；
2. 内蒙古医科大学附属医院, 呼和浩特 010050

收稿日期:2015-06-12 出版日期:2016-03-24 发布日期:2016-03-25
作者简介:吕洋, 男, 博士研究生, 研究方向：哺乳动物生殖生物学；E-mail：lvyang678762006@163.com
基金资助:
国家基础研究计划(2012CB722306)；国家级大学生创新训练计划项目(201410126034)

Expression of microRNA-483 and microRNA-486 in the Cloned and fat-1-transgenic Bovine

Lü Yang¹, WANG Yu^{1, 2}, SUN Jia-jia¹, GONG Chun-ling¹, LI Guang-peng¹

1. Research Center for Laboratory Animal Science, Inner Mongolia University, Hohhot 010070；
2. The Affiliated Hospital, Inner Mongolia University, Hohhot 010050

Received:2015-06-12 Published:2016-03-24 Online:2016-03-25

摘要/Abstract

摘要： 利用荧光定量PCR比较正常受精牛、克隆牛和转基因牛的心、肝、脾、肺、肾、胎盘、子叶、子宫内膜中miR-483和miR-486的表达水平。结果显示, miR-483和miR-486在正常受精牛、克隆牛和转fat-1基因牛的组织中均有表达, 其中在心脏中表达量显著高于其他组织。而转fat-1基因牛心脏中miR-483和miR-486表达量均低于正常牛。miR-483和miR-486在不同组织中表达量存在一定差异, 在心脏中呈现高表达, 提示miR-483和miR-486表达降低可能与心肌肥大、心肌梗死等病理生理过程有关。

关键词: miR-483, miR-486, 转基因牛, 心脏

Abstract: The expression levels of microRNA-483 and microRNA-486 in heart, liver, spleen, lung, kidney, placenta, cotyledons, endometrium from normal, cloned, and transgenic bovine were measured by real time quantitative PCR. The results showed that microRNA-483 and microRNA-486 were expressed in all tissues of normal, cloned and fat-1-transgenic bovine；significantly higher in the heart than other tissues. However, the expression levels of microRNA-483 and microRNA-486 in transgenic bovine were lower than normal one. Further, the expressions of microRNA-483 and microRNA-486 varied in different tissues and high in the heart, indicating that the expressions of microRNA-483 and microRNA-486 may be correlated with pathophysiological process of myocardial hypertrophy and myocardial infarction.

Key words: microRNA-483, microRNA-486, transgenic bovine, heart

吕洋,王煜,孙佳佳,弓春玲,李光鹏. microRNA-483和microRNA-486在克隆和转fat-1基因牛组织中的表达[J]. 生物技术通报, 2016, 32(3): 105-108.

Lü Yang, WANG Yu, SUN Jia-jia, GONG Chun-ling, LI Guang-peng. Expression of microRNA-483 and microRNA-486 in the Cloned and fat-1-transgenic Bovine[J]. Biotechnology Bulletin, 2016, 32(3): 105-108.

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microRNA-483和microRNA-486在克隆和转fat-1基因牛组织中的表达

Expression of microRNA-483 and microRNA-486 in the Cloned and fat-1-transgenic Bovine

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