戊型肝炎病毒长爪沙鼠感染模型的初步研究

doi:10.13560/j.cnki.biotech.bull.1985.2016.05.032

生物技术通报 ›› 2016, Vol. 32 ›› Issue (5): 240-245.doi: 10.13560/j.cnki.biotech.bull.1985.2016.05.032

戊型肝炎病毒长爪沙鼠感染模型的初步研究

朱光泽¹，², 屈勇刚²，³, 李一权², 兰添², 范园园², 胡宁宁², 张诺娜², 李霄², 金宁一²

1.长春中药大学附属医院检验科，长春 130021;
2.军事医学科学院军事兽医研究所，长春 130122;
3.石河子大学动物科技学院，石河子 832003

收稿日期:2015-07-20 出版日期:2016-05-25 发布日期:2016-05-27
作者简介:朱光泽，男，博士，教授，研究方向：分子病毒学；E-mail：zhuguangze820@126.com

A Preliminary Study on Infection Model of Mongolian Gerbil by Hepatitis E Virus

ZHU Guang-ze¹，²,QU Yong-gang²，³,LI Yi-quan²,LAN Tian²,FAN Yuan-yuan²,HU Ning-ning²,ZHANG Nuo-na²,LI Xiao²,JIN Ning-yi²

1. The Affiliated Hospital of Changchun University of Traditional Chinese Medicine，Changchun 130021;
2. Institute of Veterinary Science，The Academy of Military Medical Sciences，Changchun 130122;
3. College of Animal Science and Technology，Shihezi University，Shihezi 832003

Received:2015-07-20 Published:2016-05-25 Online:2016-05-27

摘要/Abstract

摘要： 旨在探索应用长爪沙鼠（Meiiones unguiculataus）构建戊型肝炎病毒（hepatitis E virus，HEV）动物模型的可行性，从猪粪便稀释悬液中提取HEV RNA并鉴定，经腹腔和口服接种HEV于长爪沙鼠，并每周采集血液、相关器官（心、肝、脾、肺、肾、肠系膜淋巴结、脾脏）及粪便。用反转录PCR（RT-PCR）和免疫组化等检测方法分析各个样品。结果表明，感染后第7天开始，可从血液、肾脏、脾脏、肠系膜淋巴结、肝脏、肺、粪便中检测到HEV RNA；通过免疫组化和组织病理学观察，发现HEV可在肝脏中进行表达，并且能引起肝组织病理变化，但感染HEV后长爪沙鼠均缺乏较明显的临床表现症状。结果提示，长爪沙鼠对 HEV具有易感性，感染后肝脏出现小叶性肝炎的病理变化，具有作为HEV感染动物模型的潜在价值。

关键词: 戊型肝炎病毒, 长爪沙鼠, 动物模型

Abstract: To explore the feasibility of using Mongolian gerbils（Meiiones unguiculataus）to construct an animal model of hepatitis E virus（HEV），HEV RNA was extracted from the diluted suspension of swine feces，then Mongolian gerbils were experimentally inoculated with the HEV via intraperitoneal and oral. Subsequently，the samples of blood，relevant organs（heart，liver，spleen，lungs，kidneys，mesenteric lymph nodes，and spleen）and feces were collected weekly. Samples were detected by RT-PCR and immunohistochemistry. As a result，from day 7 after infection，HEV RNA was detected from the blood，kidney，spleen，mesenteric lymph nodes，liver，lung and feces. The analysis of immunohistochemistry discovered that HEV was expressed in the liver，and caused the pathological changes in liver tissue，but it was lack of obvious clinical manifestations in Mongolian gerbils after HEV infection. All above results suggest that Mongolian gerbils have susceptibility to HEV，and have pathological changes of lobular hepatitis in the liver after HEV infection，indicating that Mongolian gerbils have a potential value to be the animal model of HEV infection.

Key words: hepatitis E virus, Mongolian gerbils, animal model

朱光泽, 屈勇刚, 李一权, 兰添, 范园园, 胡宁宁, 张诺娜, 李霄, 金宁一. 戊型肝炎病毒长爪沙鼠感染模型的初步研究[J]. 生物技术通报, 2016, 32(5): 240-245.

ZHU Guang-ze,QU Yong-gang,LI Yi-quan,LAN Tian,FAN Yuan-yuan,HU Ning-ning,ZHANG Nuo-na,LI Xiao,JIN Ning-yi. A Preliminary Study on Infection Model of Mongolian Gerbil by Hepatitis E Virus[J]. Biotechnology Bulletin, 2016, 32(5): 240-245.

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戊型肝炎病毒长爪沙鼠感染模型的初步研究

A Preliminary Study on Infection Model of Mongolian Gerbil by Hepatitis E Virus

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