定量蛋白质组学在动物睾丸蛋白研究中的应用进展

摘要/Abstract

摘要： 蛋白质组学是后基因时代重要的研究领域之一。而定量蛋白质组学是蛋白质组学研究的主要方法之一，可以反映蛋白质的动态本质。睾丸作为雄性动物重要的生殖器官，具有产生精子和分泌雄激素的功能。因此，应用蛋白质组学方法来探索睾丸中蛋白质的组成具有重要意义。就定量蛋白质组学的概念、研究技术手段及其在动物睾丸发育、疾病和各种应激条件下等方面的应用进行综述，旨在为睾丸蛋白质的进一步研究提供参考。

关键词: 定量蛋白质组学, 睾丸, 动物, 进展

Abstract: Proteomics is one of the most important research areas at post-genomics era and quantitative proteomics is one of the main methods to study proteomics，which may reflect the dynamic nature of proteins. As a key male reproductive organ，testis produces sperms and secrets androgens. Thus it is of great significance to investigate the protein compositions of testis by the method of proteomics. In this review，we mainly focus on the concept of proteomics and the technological means of quantitative proteomics，as well as its application in the studies of testicular development，and responses to diseases and various stress conditions, stress conditions which provids insight into the further research on protein in testis.

Key words: quantitative proteomics, testis, animal, advances

中图分类号:

10.13560/j.cnki.biotech.bull.1985.2016.12.002

卢曾奎,马友记，. 定量蛋白质组学在动物睾丸蛋白研究中的应用进展[J]. 生物技术通报, 2016, 32(12): 8-12.

LU Zeng-kui,MA You-ji，. Application Advances of Quantitative Proteomics in the Studies of Animal Testis Protein[J]. Biotechnology Bulletin, 2016, 32(12): 8-12.

参考文献

[1] Desjardins C. Endocrine regulation of reproductive development and function in the male[J] . J Anim Sci, 1978, 47（Suppl 2）：56-79.
[2] Amini MR, Kohram H, Shahaneh AZ, et al. The effects of different levels of vitamin E and vitamin C in modified Beltsville extender on rooster post-thawed sperm quality[J] . Cell & Tissue Banking, 2015, 16（4）：1-6.
[3] Polguj M, Wysiadecki G, Podgórski M, et al. Morphological variati-ons of intra-testicular arterial vasculature in bovine testis-a corrosion casting study[J] . BMC Vet Res, 2015, 11（263）：1-6.
[4] Ali Mohammed P, Itaru M, Naoto U, et al. Expression of insulin-like factor 3 hormone-receptor system in the reproductive organs of male goats[J] . Cell & Tissue Research, 2015, 362（2）：407-420.
[5] Yulema V, Miriam SH, Alicia GA, et al. Characterization of the annual regulation of reproductive and immune parameters on the testis of European sea bass[J] . Cell & Tissue Research, 2015, 362（1）：215-229.
[6] Wasinger VC, Cordwell SJ, Cerpa-Poljak A, et al. Progress with gene-product mapping of the Mollicutes：Mycoplasma genitalium[J] . Electrophoresis, 1995, 16（1）：1090-1094.
[7] Dong SW, Zhang SD, Wang DS, et al. Comparative proteomics analysis provide novel insight into laminitis in Chinese Holstein cows[J] . Bmc Veterinary Research, 2015, 11（1）：162-169.
[8] Ahn JY, Kim IY, Oh SJ, et al. Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry[J] . Lab Anim Res, 2014, 30（2）：45-53.
[9] Gao XG, Wu W, Ma CG, et al. Postmortem changes in sarcoplasmic proteins associated with color stability in lamb muscle analyzed by proteomics[J] . Eur Food Res Technol, doi：10.1007/s00217-015-2563-2.
[10] Yang YX, Bu DP, Zhao XW, et al. Proteomic analysis of cow, yak, buffalo, goat and camel milk whey proteins：quantitative differential expression patterns[J] . Journal of Proteome Research, 2013, 12（4）：1660-1667.
[11] Sun CJ, Xu GY, Yang N. Differential label-free quantitative proteomic analysis of avian eggshell matrix and uterine fluid proteins associated with eggshell mechanical property[J] . Proteomics, 2013, 13（23-24）：3523-3536.
[12] O’Farrell PH. High resolution two-dimensional electrophoresis of proteins[J] . J Biol Chem, 1975, 250（10）：4007-4021.
[13] Sheoran IS, Ross ARS, Olson DJH, et al. Compatibility of plant protein extraction methods with mass spectrometry for proteome analysis[J] . Plant Science, 2009, 176（1）：99-104.
[14] Pomastowski P, Buszewski B. Two-dimensional gel electrophoresis in the light of new developments[J] . Trends in Analytical Chemistry, 2014, 53：167-177.
[15] Donnell R, Holland JW, Deeth HC, et al. Milk Proteomics[J] . International Dairy Journal, 2004, 14（12）：1013-1023.
[16] Wilkins MR, Appel RD, Eyk JEV, et al. Guidelines for the next 10 years of proteomics[J] . Proteomics, 2006, 6（1）：4-8.
[17] 赵楠, 王桂媛, 王玲姝, 等. 蛋白质组学关键技术研究进展[J] . 生物技术通讯, 2011, 22（4）：580-583.
[18] Cossio G, Sanchez JC, Wettstein R, et al. Spermatocytes and round spermatids of rat testis：the difference between in vivo and in vitro protein patterns[J] . Electrophoresis, 1997, 18（3-4）：548-552.
[19] Witzmann FA, Fultz CD, Wyman JF. Two-dimensional electrophoresis of precision-cut testis slices：Toxicologic application[J] . Electrophoresis, 1997, 18（3-4）：642-646.
[20] 朱叶飞, 马翔, 周作民, 等. 双向凝胶电泳和质谱技术在睾丸蛋白表达研究中的应用[J] . 中华男科学杂志, 2003, 9（2）：85-89.
[21] Huang SY, Lin JY, Chuang CK, et al. A reference map and identification of porcine testis proteins using 2-DE and MS. [J] . Proteomics, 2005, 5（16）：4205-4212.
[22] Paz M, Morín M, Mazo JD. Proteome profile changes during mouse testis development[J] . Comparative Biochemistry & Physiology Part D Genomics & Proteomics, 2006, 1（4）：404-415.
[23] 霍然. 人睾丸蛋白质表达谱的构建及精子发生相关蛋白质组学研究[D] . 南京：南京医科大学, 2006.
[24] Li JY, Liu FJ, Liu X, et al. Mapping of the human testicular prote-ome and its relationship with that of the epididymis and spermato-zoa. [J] . Mol Cell Proteomics Mcp, 2011, 10（3）：318-324.
[25] Huang SY, Lin JH, Teng SH, et al. Differential expression of porcine testis proteins during postnatal development[J] . Animal Reproduction Science, 2011, 123（3-4）：221-233.
[26] 卢曾奎, 张全伟, 马友记, 等. 绵羊睾丸蛋白质双向电泳体系的建立及优化[J] . 农业生物技术学报, 2015, 23（12）：1660-1666.
[27] 蒋荣江, 沈华, 沈百欣, 等. 尿道下裂大鼠与正常大鼠睾丸蛋白质二维电泳图谱分析[J] . 南京医科大学学报：自然科学版, 2007, 27（12）：1363-1365.
[28] Huo R, He Y, Zhao C, et al. Identification of human spermatogen-esis-related proteins by comparative proteomic analysis：a prelim-inary study[J] . Fertil Steril, 2008, 4：1109-1118.
[29] 李飞, 邹亚光, 赵亮, 等. 成年无精子症男性睾丸活检组织双向电泳分析技术的初步建立及其优化[J] . 实用医学杂志, 2009, 25（24）：4139-4142.
[30] 张建鹏, 任绪义, 郭婷婷, 等. 大鼠睾丸镉毒性相关蛋白质组学研究[J] . 生物技术通讯, 2005, 16（4）：357-362.
[31] 陈浩宇, 王水明, 彭瑞云, 等. 微波辐射致睾丸早期损伤的蛋白质组学研究[J] . 解放军医学杂志, 2009, 11：1304-1307.
[32] Ma XM, Chen X, Ma SW, et al. Protein expression profile in the testis of rats chronically exposed to low-dose 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin[J] . Toxicological & Environmental Chemistry, 2010, 92（9）：1715-1727.
[33] 赵珺. 高温热应激环境对雄性小鼠生殖机能影响及其分子机理的研究[D] . 长春：东北师范大学, 2010.
[34] 黄礼元. 吸烟小鼠睾丸蛋白组差异研究：PEBP1对吸烟后小鼠睾丸精子发生功能影响的可能机制[D] . 上海：上海交通大学, 2012.
[35] Li HY, He YX, Zhang H, et al. Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes. [J] . Toxicology Letters, 2014, 225（3）：433-444.