溶血磷脂酸对不同转移性肝癌细胞迁移行为的影响

生物技术通报 ›› 2016, Vol. 32 ›› Issue (12): 189-194.

溶血磷脂酸对不同转移性肝癌细胞迁移行为的影响

李铎,李佳文,袁群琛,林川川,宋关斌

重庆大学生物工程学院，重庆 400044

收稿日期:2016-04-20 出版日期:2016-12-25 发布日期:2016-12-07
作者简介:李铎，女，研究方向：细胞生物学；E-mail：liduo1995@live.com
基金资助:
国家自然科学基金项目（11272365），国家级大学生创新训练项目（201510611045）

Effects of Lysophosphatidic Acid on the Migrations of Hepatoma Cells with Different Metastasis

LI Duo,LI Jia-wen,YUAN Qun-chen,LIN Chuan-chuan,SONG Guan-bin

College of Bioengineering，Chongqing University，Chongqing 400044

Received:2016-04-20 Published:2016-12-25 Online:2016-12-07

摘要/Abstract

摘要： 旨在研究溶血磷脂酸（Lysophosphatidic acid，LPA）对两种转移性不同的肝癌细胞MHCC97H和HepG2迁移行为的影响。采用Transwell法检测肝癌细胞的迁移能力，RT-PCR检测肝癌细胞中LPA受体（LPA receptor，LPAR）mRNA水平的表达。结果显示，LPA对低转移性肝癌细胞HepG2的迁移没有明显影响，但显著促进高转移性肝癌细胞MHCC97H的迁移能力。LPA对两种细胞的增殖都没有明显影响。RT-PCR实验发现两种肝癌细胞中LPAR的表达呈现差异，LPAR1在MHCC97H细胞中表达，但HepG2细胞不表达。用LPAR1/3抑制剂Ki16425阻断MHCC97H中LPAR1的作用后发现，LPA对MHCC97H细胞的促迁移作用消失，表明LPA通过与LPAR1作用促进了MHCC97H细胞的迁移。LPA对不同转移性肝癌细胞迁移能力的影响存在差异，该差异可能来自于细胞表达LPAR的不同。

关键词: 溶血磷脂酸, 肝癌细胞, 溶血磷脂酸受体, 细胞迁移

Abstract: The aim of this study is to investigate the effect of lysophosphatidic acid（LPA）on cell migration of two kinds of hepatocellular carcinoma cells（MHCC97H and HepG2）with different metastasis，and to evaluate the underlying mechanism. Transwell assay was employed to investigate the impact of LPA on the migration of the hepatocellular carcinoma cells，and RT-PCR to detect the mRNA expression of LPA receptor（LPAR）in the hepatocellular carcinoma cells. Results showed that the LPA presented no obvious effect on the migration of HepG2 cells in low metastasis，but significantly promoted the migration of MHCC97H cells in high metastasis. LPA caused insignificant impact to the proliferation of both cells. RT-PCR demonstrated that expressions of LPAR at mRNA level in MHCC97H and HepG2 cells were different，LPAR1 expressed in MHCC97H cells，however，not in HepG2 cells. Importantly，the promotion effect of LPA on the migration of MHCC97H cells disappeared after Ki16425（a specific inhibitor of LPAR1/3）was used to block the role of LPAR1 in MHCC97H cells. This indicated that LPA promoted MHCC97H cell migration via interaction with LPAR1. The effects of LPA on the migration of hepatoma cells in different metastasis varied，which resulted from the different expressions of LPAR in these two cell types.

Key words: lysophosphatidic acid, hepatoma cell, lysophosphatidic acid receptor, cell migration

中图分类号:

10.13560/j.cnki.biotech.bull.1985.2016.12.029

李铎,李佳文,袁群琛,林川川,宋关斌. 溶血磷脂酸对不同转移性肝癌细胞迁移行为的影响[J]. 生物技术通报, 2016, 32(12): 189-194.

LI Duo,LI Jia-wen,YUAN Qun-chen,LIN Chuan-chuan,SONG Guan-bin. Effects of Lysophosphatidic Acid on the Migrations of Hepatoma Cells with Different Metastasis[J]. Biotechnology Bulletin, 2016, 32(12): 189-194.

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