生物技术通报 ›› 2017, Vol. 33 ›› Issue (12): 67-73.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0467

• 技术与方法 • 上一篇    下一篇

高通量基因型分型技术及其在水稻中的应用

张先文1,2,3, 贺治洲2, 江南2, 邓华凤1, 李继明2   

  1. 1. 杂交水稻国家重点实验室 湖南杂交水稻研究中心 湖南省农业科学院,长沙 410125;
    2. 华智水稻生物技术有限公司,长沙 410125;
    3. 湖南农 业大学生物科学技术学院,长沙 410128
  • 收稿日期:2017-06-05 出版日期:2017-12-25 发布日期:2017-12-21
  • 作者简介:张先文,男,博士研究生,研究方向:水稻杂种优势;E-mail:xianwenzhang@hunau.edu.cn
  • 基金资助:
    国家自然科学基金项目(31000125),中国博士后基金(2016M600627),杂交水稻国家重点实验室(湖南杂交水稻研究中心)开放课题基金(2016KF05)

High-throughput Genotyping Techniques and Their Applications in Rice

ZHANG Xian-wen1, 2, 3, HE Zhi-zhou2, JIANG Nan2, DENG Hua-feng1, Li Ji-ming2   

  1. 1. State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410125;
    2. Huazhi Rice Biotech Co.,Ltd.,Changsha 410125;
    3. College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128
  • Received:2017-06-05 Published:2017-12-25 Online:2017-12-21

摘要: 作物种质资源遗传基础的深入认识与高效利用,对于品种改良和粮食安全具有重要意义。传统系谱考察的方法对指导育种实践发挥了重要作用,随着分子生物学的发展,基因组学和高通量SNP分子标记等基因型分型方法可以更便捷地对种质资源进行鉴定。就基因型分型的主要方法进行了总结,重点论述了以SNP为核心的下一代高通量测序(NGS)分型方法、竞争性等位基因特异性PCR(KASP)和SNP芯片系统,以及当前主流的SNP分析工具和数据库。同时,介绍了高通量SNP分型技术在水稻研究中的进展,并就分型技术在作物育种等领域的应用和发展趋势进行了展望。

关键词: 系谱, SNP, 分型, 下一代高通量测序, 竞争性等位基因特异性PCR

Abstract: Deep understanding and efficient utilization of genetic basis of crop germplasm resources is of great importance for crop cultivar improvement and food safety. The traditional methods based on pedigree played a vital role in breeding practice. With the rapid development of molecular biology,genotyping methods based on genomics and high-throughput SNP markers have been developed and may efficiently identify the germplasm resources. This article reviews the main genotyping methods,focusing on the methods based on single nucleotide polymorphism(SNP)marker such as the next-generation sequencing(NGS),competitive allele-specific PCR(KASP),and SNP chip as well as the popular SNP analysis tools and databases. Furthermore,this article introduces the progresses of high-throughput SNP genotyping techniques in studying rice,and prospects the application and developmental trends of genotyping techniques in the fields such as crop breeding and so on.

Key words: pedigree, SNP, genotyping, next generation sequencing, competitive allele-specific PCR