三种DNA指纹图谱技术在菌株分型中的应用

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.012

生物技术通报 ›› 2015, Vol. 31 ›› Issue (6): 81-86.doi: 10.13560/j.cnki.biotech.bull.1985.2015.06.012

三种DNA指纹图谱技术在菌株分型中的应用

刘毅姚粟李辉刘洋刘勇刘波程池

中国食品发酵工业研究院中国工业微生物菌种保藏管理中心，北京 100015

收稿日期:2014-11-20 出版日期:2015-06-19 发布日期:2015-06-20
作者简介:刘毅，男，硕士，研究方向：微生物学；E-mail：lewisbeyond@163.com

Application of 3 DNA Fingerprinting Techniques in Strain Identification

Liu Yi, Yao Su, Li Hui, Liu Yang, Liu Yong, Liu Bo, Cheng Chi

China National Research Institute of Food and Fermentation Industries，China Center of Industrial Culture Collection，Beijing 100015

Received:2014-11-20 Published:2015-06-19 Online:2015-06-20

摘要/Abstract

摘要： 旨在通过ERIC-PCR、BOX-PCR、RAPD等技术对4株地芽孢杆菌属菌株进行DNA指纹图谱分析，找到一种适合于地芽孢杆菌属尤其是嗜热脂肪地芽孢杆菌的菌株分型方法。4株地芽孢杆菌属菌株呈现出4种不同的指纹图谱，其中ERIC-PCR的方法获得的条带较为清晰，实验方法较为简便快捷，且能相对较好地区分不同株系；BOX-PCR的方法得到的条带相对较少，不能很好地区分同一种菌的不同株系；RAPD的方法获得的条带相对较多，区分性更高，但同时也增加了一部分工作量和成本。3种菌株分型技术均能有效的区分地芽孢杆菌属菌株，其中ERIC-PCR是一种最有效最便捷区分嗜热脂肪地芽孢杆菌不同株系的方法。

关键词: 菌株分型, ERIC-PCR, BOX-PCR, RAPD, 嗜热脂肪地芽孢杆菌

Abstract: Four strains of Geobacillus were analyzed with 3 DNA fingerprinting techniques of ERIC-PCR, BOX-PCR and RAPD for searching the proper strain identification measure for the genus especially Geobacillus stearothermophilus. Each of 4 strains of G. stearothermophilus had different fingerprint. The bands by simple and quick ERIC-PCR were quite clear, and the different strains could be recognized clearly. The bands by BOX-PCR were less, and the strains of the same bacteria could not be recognized clearly. More bands could be obtained by RAPD with higher identification；however the workload of identification and cost increased significantly. Three techniques may effectively identify the strains of Geobacillus, and ERIC-PCR is the most effective and fast method to identify strains of G. stearothermophilus.

Key words: strain identification, ERIC-PCR, BOX-PCR, RAPD, Geobacillus stearothermophilus

刘毅, 姚粟, 李辉, 刘洋, 刘勇, 刘波, 程池. 三种DNA指纹图谱技术在菌株分型中的应用[J]. 生物技术通报, 2015, 31(6): 81-86.

Liu Yi, Yao Su, Li Hui, Liu Yang, Liu Yong, Liu Bo, Cheng Chi. Application of 3 DNA Fingerprinting Techniques in Strain Identification[J]. Biotechnology Bulletin, 2015, 31(6): 81-86.

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三种DNA指纹图谱技术在菌株分型中的应用

Application of 3 DNA Fingerprinting Techniques in Strain Identification

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