鲤在低温胁迫下肝胰腺转录组测序分析

doi:10.13560/j.cnki.biotech.bull.1985.2018-0503

生物技术通报 ›› 2018, Vol. 34 ›› Issue (11): 168-178.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0503

鲤在低温胁迫下肝胰腺转录组测序分析

刘思嘉^1,2, 田菲¹, 张存芳¹, 乔志刚³, 赵凯¹

1. 中国科学院西北高原生物研究所,西宁 810008;
2. 中国科学院大学,北京 100049;
3. 河南师范大学水产学院,新乡 453007

收稿日期:2018-05-31 出版日期:2018-11-26 发布日期:2018-11-28
作者简介:刘思嘉,男,博士研究生,研究方向：功能基因组学;E-mail：liusijia_1022@163.com
基金资助:
中国科学院先导项目A类（XDA08010208-3）

Transcriptome Sequencing and Analysis of Hepatopancreas from Carps Under Cold Stress

LIU Si-jia^1,2, TIAN Fei¹, ZHANG Cun-fang¹, QIAO Zhi-gang³, ZHAO Kai¹

1. Key Laboratory of Adaptation and Evolution of Plateau Biota,and Laboratory of Plateau Fish Evolutionary and Functional Genomics,and Qinghai Key Laboratory of Animal Ecological Genomics,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008;
2. University of Chinese Academy of Sciences,Beijing 100049;
3. College of Fisheries Henan Normal University,Xinxiang 453007

Received:2018-05-31 Published:2018-11-26 Online:2018-11-28

摘要/Abstract

摘要： 检测低温耐受与低温敏感鲤品种低温胁迫下的差异表达基因,探究鲤低温适应的分子应答过程。低温胁迫后对松浦镜鲤及荷包红鲤肝胰腺进行转录组测序,并对表达差异基因进行功能、通路富集分析。通过差异表达分析,共获得10 521个差异表达基因,其中5 246个基因仅在低温耐受品种发生表达变化。功能、通路富集分析将差异基因显著富集在包括糖酵解/糖质新生代谢途径在内的144个代谢途径。选取5个基因进行qRT-PCR验证,定量结果与RNA-seq结果相关性达0.89。研究结果表明低温胁迫会显著改变鲤肝胰腺的转录活动,编码糖酵解/糖质新生途径的多个关键酶基因在低温耐受品种的表达量显著高于低温敏感品种,推测这些基因功能有助于其长期低温适应。

关键词: 鲤, 低温耐受, 肝胰腺, 转录组测序, 糖酵解/糖质新生途径

Abstract: This work aims to detect the differentially-expressed genes（DEGs）of cold-tolerant and cold-sensitive carps and to understand the molecular response mechanisms for carps to adapt in cold environment. The transcriptome sequencing of hepatopancreas from cold-tolerant Songpu carp and a cold-sensitive Hebao carp were conducted and the enrichment analysis of DEG function and pathways were carried out. Totally,10 521 DEGs were detected via differentially-expressed analysis,and 5246 DEGs of them presented expression in cold-tolerant species. DEGS were enriched in 144 metabolic pathways including glycolysis/gluconeogenesis pathway. Five DEGs were selected to conduct qRT-PCR,and the correlation of the results between qRT-PCR and RNA-seq was 0.89.This study revealed that cold stress significantly changed the transcriptome of hepatopancreas in the experimental carp. The expressions of genes encoding the key enzymes of glycolysis/gluconeogenesis pathway were apparently higher than that in cold-sensitive ones,revealing that the functions of these genes facilitate the long-term cold-adaptation of the cold-tolerant carps.

Key words: Cyprinus carpio, cold tolerance, hepatopancreas, transcriptome sequencing, glycolysis/gluconeogenesis pathway

刘思嘉, 田菲, 张存芳, 乔志刚, 赵凯. 鲤在低温胁迫下肝胰腺转录组测序分析[J]. 生物技术通报, 2018, 34(11): 168-178.

LIU Si-jia, TIAN Fei, ZHANG Cun-fang, QIAO Zhi-gang, ZHAO Kai. Transcriptome Sequencing and Analysis of Hepatopancreas from Carps Under Cold Stress[J]. Biotechnology Bulletin, 2018, 34(11): 168-178.

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鲤在低温胁迫下肝胰腺转录组测序分析

Transcriptome Sequencing and Analysis of Hepatopancreas from Carps Under Cold Stress

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