1株产生洛伐他汀杂色曲霉的筛选、鉴定和发酵优化条件

doi:10.13560/j.cnki.biotech.bull.1985.2018-0453

生物技术通报 ›› 2018, Vol. 34 ›› Issue (11): 210-215.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0453

1株产生洛伐他汀杂色曲霉的筛选、鉴定和发酵优化条件

许楚旋, 王嘉琦, 蒋冬花

浙江师范大学化学与生命科学学院,金华 321004

收稿日期:2018-05-15 出版日期:2018-11-26 发布日期:2018-11-28
作者简介:许楚旋,女,研究方向：微生物资源;E-mail：651831034@qq.com
基金资助:
国家自然科学基金项目（31570013）,国家级大学生创新创业训练计划项目（201710345014）,浙江省大学生科技创新活动计划暨新苗人才计划项目（2018R404026）,浙江省公益技术研究计划项目（GN18C010004）

Screening and Identification of a Lovastatin-producing Strain of Aspergillus versicolor and Preliminary Optimization of Fermentation Conditions

XU Chu-xuan, WANG Jia-qi, JIANG Dong-hua

College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004

Received:2018-05-15 Published:2018-11-26 Online:2018-11-28

摘要/Abstract

摘要： 从不同生境收集的自然发酵样品中分离纯化获86株曲霉纯菌株;用薄层层析法（TLC）和高效液相色谱法（HPLC）测定曲霉各菌株发酵液中洛伐他汀（Lovastatin）含量进行筛选;获1株较高产Lovastatin的编号为A-8曲霉菌株,产量为58 g/L。根据形态特征和ITS基因序列,鉴定A-8菌株为杂色曲霉。单因素实验初步优化了A-8曲霉菌株发酵条件和培养基配方,结果表明A-8菌株产生Lovastatin较适宜的发酵条件为：温度28℃、初始pH 5.2、摇床转速180 r/min、接种量为10%;培养基配方为：乳糖为碳源、含量为100 g/L,蛋白胨为氮源、含量为12 g/L,碳源/氮源为15∶1.5;在该条件下A-8曲霉菌株发酵产生Lovastatin的水平最高,可达130.04 μg/mL,比优化前提高了约2.24倍。A-8曲霉菌株是较有潜力的工业菌株。

关键词: 杂色曲霉, 洛伐他汀, 筛选, 鉴定, 优化

Abstract: Total 86 Aspergillus strains were selected from different natural fermentation samples of food,soil,air and so on. Lovastatin yields of Aspergillus strains were measured by thin-layer chromatography（TLC）and high performance liquid chromatography（HPLC）methods. An Aspergillus strain（No. A-8）producing higher lovastatin was screened out,and the yield was of 58 g/L. Strain A-8 was identified as Aspergillus versicolor based on ITS sequence and morphology characteristics. Medium components and fermentation conditions of Aspergillus strain A-8 were optimized using single factor experiment. The results indicated that appropriate fermentation conditions were 28℃,initial pH 5.2,rotation speed 180 r/min,and inoculation size 10%. Optimal medium components were as follows：C source lactose,C content 100 g/L,N source peptone,N content 12 g/L,C/N 15：1.5. The lovastatin production of Aspergillus strain A-8 increased by 2.24 times and resulted in 130.04 μg/mL. The Aspergillus strain A-8 is one potential industrial strain.

Key words: Aspergillus versicolor, lovastatin, screening, identification, optimization

许楚旋, 王嘉琦, 蒋冬花. 1株产生洛伐他汀杂色曲霉的筛选、鉴定和发酵优化条件[J]. 生物技术通报, 2018, 34(11): 210-215.

XU Chu-xuan, WANG Jia-qi, JIANG Dong-hua. Screening and Identification of a Lovastatin-producing Strain of Aspergillus versicolor and Preliminary Optimization of Fermentation Conditions[J]. Biotechnology Bulletin, 2018, 34(11): 210-215.

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1株产生洛伐他汀杂色曲霉的筛选、鉴定和发酵优化条件

Screening and Identification of a Lovastatin-producing Strain of Aspergillus versicolor and Preliminary Optimization of Fermentation Conditions

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