施陶丁格连接介导的生物传感及药物运输研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-0096

摘要/Abstract

摘要： 施陶丁格连接是有机叠氮化物与膦在室温、水溶液等温和条件下直接发生的一种无金属催化的点击反应。由于施陶丁格连接具有生物正交特性且无潜在的细胞毒性,目前正广泛应用于材料表面功能化、各种生物大分子的合成及标记等方面。同时,在药物的合成与递送以及生物传感器等应用中具有较大发展空间。详细介绍了施陶丁格连接的反应机理、影响反应动力学、连接产率以及反应进程的多种因素,以及该连接反应介导的生物标记技术。在此基础上,论述了施陶丁格连接在生物传感中的应用,包括以核酸、小分子为靶标的荧光生物传感器;细胞成像技术在核酸、聚糖中的应用;以及在药物合成与递送中的应用。最后预测了施陶丁格连接未来的发展方向以及在生物传感中的应用前景。

关键词: 施陶丁格连接, 点击化学, 生物传感, 药物运输, 细胞成像

Abstract: The Staudinger ligation is a type of metal-free catalyzed click reaction between organic azide and phosphine under the mild conditions like at room temperature and in the water solution. Due to its bio-orthogonality and no potential cytotoxicity,Staudinger ligation is now widely used in the surface functionalization of materials,synthesis and labelling of various biological macromolecules. At the same time,there is great development space for applying Staudinger ligation in the synthesis and delivery of drugs and biosensors. In this review,the Staudinger ligation is described in detail involving the reaction mechanism,the factors influencing the reaction kinetics,the yield of the ligation and the reaction process,and the biomarker technology mediated by the ligation. On these bases,the biosensing applications mediated by the Staudinger ligation are discussed,including fluorescent biosensors targeting nucleic acids and small molecules,the applications of cell imaging technology in nucleic acid and glycan,and the applications in drug synthesis and delivery. At last,the future development trend of the Staudinger ligation is forecasted and the application prospects in the biosensing are presented.

Key words: Staudinger ligation, click chemistry, biosensing, drug delivery, cell imaging

张洋子, 朱龙佼, 邵向丽, 许文涛. 施陶丁格连接介导的生物传感及药物运输研究进展[J]. 生物技术通报, 2019, 35(1): 187-198.

ZHANG Yang-zi, ZHU Long-jiao, SHAO Xiang-Li, XU Wen-tao. Research Progress on Biosensing and Drug Delivery Mediated by the Staudinger Ligation[J]. Biotechnology Bulletin, 2019, 35(1): 187-198.

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