右旋糖酐酶开发及应用研究进展

doi:10.13560/j.cnki.biotech.bull.1985.2018-1021

摘要/Abstract

摘要： 右旋糖酐酶是一类能特异性催化右旋糖酐中α-1,6-糖苷键水解的酶,其作用底物——右旋糖酐则是蔗糖经某些微生物发酵生成的高分子葡萄糖聚合物。右旋糖酐被广泛应用于医药、食品、材料等领域,但也给口腔健康、制糖生产等带来不良影响。随着人们对右旋糖酐、右旋糖酐水解物及其他多糖研究的深入,右旋糖酐酶发挥着越来越重要的作用,但右旋糖酐酶制剂的整体开发水平仍不高,应用深度仍有限。综述了右旋糖酐酶菌种构建、发酵、纯化、固定化、酶学性质表征、酶活性增强等多个方面的开发研究,以及右旋糖酐酶在制糖、食品、医药、材料等领域的应用研究进展,其中包括本团队在开发和应用方面的研究成果,讨论了在开发和应用过程中存在的问题,并对未来的开发和应用研究方向进行了展望。

关键词: 右旋糖酐酶, 右旋糖酐, 生物被膜, 制糖, 血浆代用品

Abstract: Dextranase is a kind of enzyme that can specifically catalyzes the hydrolysis of α-1,6-glucosidic bonds on dextran. Dextran,the substrate of dextranase,is a glucan produced by the fermentation of sucrose via some microorganisms. Dextran is widely used in medicine,food,materials and other fields,but it brings the adverse effects on oral health,sugar production,etc. Studies on dextran,dextran hydrolysates and other polysaccharides are getting increasingly advanced and dextranase plays an increasingly important role;but the overall development level of dextranase preparation is still not high and its application is still limited. This review summarizes the development and researches on the construction of dextranase-producing strains,fermentation and purification of dextranase,dextranase immobilization,characterization of enzymatic properties,enzyme activity enhancement,etc.,as well as the research progresses on applying dextranase in sugar production,food,medicine and materials,including the research results in our team. Then the paper discusses the issues in the development and application and prospects the future research and development directions.

Key words: dextranase, dextran, biofilm, sugar manufacturing, plasma substitute

常国炜, 黄曾慰, 黎志德, 梁达奉. 右旋糖酐酶开发及应用研究进展[J]. 生物技术通报, 2019, 35(6): 196-204.

CHANG Guo-wei, HUANG Zeng-wei, LI Zhi-de, LIANG Da-feng. Research Progress on the Development and Application of Dextranase[J]. Biotechnology Bulletin, 2019, 35(6): 196-204.

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