雷州黑鸭FSHR和ESR1与繁殖性状关联分析

doi:10.13560/j.cnki.biotech.bull.1985.2019-0094

摘要/Abstract

摘要： 旨为探究产蛋基因FSHR和ESR1对雷州黑鸭的影响。采用荧光定量PCR对FSHR和ESR1在雷州黑鸭0-120 d的下丘脑-垂体-卵巢轴的表达规律进行研究,并通过PCR-SSCP对FSHR和ESR1的多态性与繁殖性状关联分析。结果表明,FSHR在0-90 d表达量总体呈稳定上升趋势,但在90-120 d下丘脑和垂体表达量略微下调,差异不显著（P>0.05）;ESR1表达量在0-120 d表达量总体呈上升趋势,但在90 d卵巢表达量出现波动,差异不显著（P>0.05）;FSHR的SNP（g.856937G>A,g.922947A>G）和ESR1的SNP（g.190744A>G）与雷州黑鸭产蛋量显著相关（P<0.05）,但与雷州黑鸭蛋品质关联不显著。以上结果提示：FSHR和ESR1对雷州黑鸭早期性腺发育起着重要作用,且3个SNP位点均可作为雷州黑鸭重要的产蛋标记基因。

关键词: 雷州黑鸭, FSHR, ESR1, SNP, 繁殖性状

Abstract: The aim of this study is to explore effect of the laying-related gene FSHR and ESR1 on Leizhou black duck. Quantitative PCR was employed to investigate the expression pattern of FSHR and ESR1 in hypothalamus-hypophysis-ovary axis of 0-120 d Leizhou black duck,and PCR-SSCP was used for analyzing the correlation between FSHR and ESR1 with breeding traits. The results showed that the overall expression trend of FSHR increased stably at 0-90 d and decreased slightly at 90-120 d in hypothalamus and ovary,but there was no significant difference（P>0.05）. The overall expression trend of ESR1 increased in 0-120 d but there was fluctuation at 90 d in ovary and difference was not significant（P>0.05）. The SNP for FSHR（g.856937G>A,g.922947A>G）and ESR1（g.190744A>G）were significantly associated with laying performance（P<0.05）,but were not correlated with egg quality（P>0.05）. The results suggest FSHR and ESR1 play an important role in early gonad development in Leizhou black duck,and three identified SNPs may be used as genotype maker for egg laying.

Key words: Leizhou black duck, FSHR, ESR1, SNP, breeding traits

邹坤, 崔红艳, 薛缘, 张少伟, 路丽丽, 赵志辉, 苏瑛. 雷州黑鸭FSHR和ESR1与繁殖性状关联分析[J]. 生物技术通报, 2019, 35(8): 118-126.

ZOU Kun, CUI Hong-yan, XUE Yuan, ZHANG Shao-wei, LU Li-li, ZHAO Zhi-hui, SU Ying. Correlation Between FSHR and ESR1 and Breeding Traits in Leizhou Black Duck[J]. Biotechnology Bulletin, 2019, 35(8): 118-126.

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