关键词: 雷州黑鸭, 骨骼肌, 切片, 时空表达

Abstract: To protect the excellent meat performance of Leizhou black duck,develop and utilize this precious germplasm,we selected Leizhou black duck as the experimental material and observed the tissue section of breast thigh meat in its fetal period,and used qRT-PCR and Western blot to detect the developmental expression patterns of gene Pax3,DCN and MSTN in breast thigh meat at 8th,13th,18th,23th,and 28th fetal period. Concurrently,qRT-PCR was used to test the mRNA difference expressions of genePax3,DCN and MSTN in all tissues of 28th fetal period. The observation of tissue section of breast thigh meat showed that in fetal period of Leizhou black duck,the development of thigh muscle was obviously earlier than the chest muscle. Fluorescence quantitative PCR showed that,in each stage of breast thigh meat,the relative expressions of gene Pax3,DCN and MSTN were the highest at 23th and 28th fetal period,and significantly higher than other fetal periods（P<0.05）. In all tissues of Leizhou black duck,the expressions of Pax3,DCN and MSTN in thigh muscle and chest muscle was higher than other tissues in 28th fetal period（P<0.05）;The results of Western blot showed that the expressions of Pax3,DCN and MSTN protein were the lowest in 8th fetal period and its expression in 23th fetal period was significantly higher than that in other fetal period（P< 0.05）,and reached a peak. Combined the profiles of sections,comparing them with the expression pattern analysis showed that the gene Pax3,DCN and MSTN play a determining role for the physiological characteristics of skeletal muscles in the formation and growth of muscle fibers,and regulate the differentiation,proliferation and the formation of muscle cells of Leizhou black duck early embryo at different development stages of skeletal muscle in embryonic stage.

Key words: Leizhou black duck, skeletal muscle, sliced, temporal and spatial expression