关键词: 食用菌提取物, 抗氧化活性, 抗细胞增殖活性

Abstract: In this study,the antioxidant and anti-proliferative activities of the extracts from Hypsizygus marmoreus,Lentinus edodes,Agrocybe cylindracea,Pleurotus ostreatus,Flammulina velutipes and Volvaria volvacea were evaluated. The antioxidative activities were determined by DPPH radical-scavenging,OH·radical scavenging,and total antioxidant capacity and reducing power. The anti-proliferative activities were detected by Alamar blue assay. Total phenolics and flavonoids contents of 6 edible fungi ranged from 155.76 to 283.54 mg/L and from 5.19 to 49.79 mg/L,respectively;the highest was in V. volvacea,A. cylindracea and L. edodes,while that in P.s ostreatus and H. marmoreus was the lowest. The total antioxidant capacity and reducing power of A. cylindracea and L. edodes（22.94 U/Land 20.10 U/L,0.26 and 0.233,respectively）were better than the others. The ability of scavenging DPPH· ,·OH by V. volvacea was the highest（48.8% and 75.12%,respectively）,while there was no differences among the capacities of scavenging O₂^-·. The extractions from 6 common edible mushrooms demonstrated the anti-proliferation activity against HepG2,SGC-7901,NCI-H460,MDA-MB-231 and LO2 cells. A. cylindracea showed the highest activity for HepG2 and NCI-H460（IC₅₀=4.62±2.13,4.96±1.84 mL/L）,and V. volvacea showed the highest activity for SGC-7901and MDA-MB-231（IC₅₀=5.01±1.03,6.95±1.03 mL/L）. All extractions showed low cytotoxic effect on LO2 cell. These data suggest that these 6 edible fungi appear significant antioxidative and antitumor activities in experiments in vitro,among them,P. ostreatus,L. edodes and F. velutipes have the strongest antioxidant and antitumor proliferation activities.

Key words: extractions from edible mushrooms, antioxidative activity, anti-proliferative activity