黄绿卷毛菇生境中矮嵩草内生真菌多样性比较研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-0385

生物技术通报 ›› 2019, Vol. 35 ›› Issue (11): 109-117.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0385

黄绿卷毛菇生境中矮嵩草内生真菌多样性比较研究

郭璟, 谢占玲, 罗涛, 薛治峰, 郭建娟, 李发雄, 张秀娟

青海大学生态环境工程学院青海省高原作物种质资源创新与利用重点实验室,西宁 810016

收稿日期:2019-05-07 出版日期:2019-11-26 发布日期:2019-11-19
作者简介:郭璟,女,硕士研究生,研究方向：微生物学;E-mail：guojing2146@163.com
基金资助:
国家自然科学基金项目（31560028）,青海省科技厅项目（2018-ZJ-915）

Comparative Study on Endophytic Fungi Diversity of Kobresia humilis in Floccularia luteovirens

GUO Jing, XIE Zhan-ling, LUO Tao, XUE Zhi-feng, GUO Jian-juan, LI Fa-xiong, ZHANG Xiu-juan

College of Ecology-Environment Engineering,National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm,Qinghai University,Xining 810016

Received:2019-05-07 Published:2019-11-26 Online:2019-11-19

摘要/Abstract

摘要： 为研究黄绿卷毛菇生境中矮嵩草的内生真菌多样性,本研究结合传统分离培养法和宏基因组法对矮嵩草内生真菌的多样性进行比较研究。结果显示：传统法在矮嵩草共分离鉴定得到66株内生真菌均属于子囊菌门（Ascomycota）,分类到7目,分别为格孢腔目（Pleosporales）、炭角菌目（Xylariales）、散囊菌目（Eurotiales）、柔膜菌目（Helotiales）、肉座菌目（Hypocreales）、毛霉菌目（Mucorales）、Glomerellales,优势种群为Stagonospora bicolor;根的多样性指数,丰富度指数,均匀度指数分别为1.63、1.56、0.82;未分离到黄绿卷毛菇;宏基因组法检测出587种内生真菌,子囊菌门（Ascomycota）占77.21%、担子菌门（Basidiomycota）占16.21%,剩下的6.58%为unclassified真菌,优势种群为Fusarium sp,多样性指数,丰富度指数,均匀度指数分别为3.13、39.08、0.48;检测到黄绿卷毛菇,相对丰度为11.39;比较两种方法,黄绿卷毛菇生境的矮嵩草植物中,蕴含着丰富的内生真菌资源,黄绿卷毛菇为优势内生真菌种群之一。该研究首次证明黄绿卷毛菇为嵩草属的内生真菌,是研究黄绿卷毛菇的营养类型及其与植物间的互作关系的新突破。

关键词: 矮嵩草, 内生真菌多样性, 黄绿卷毛菇, 传统培养法, 宏基因组法

Abstract: In order to study the endophytic fungi diversity of Kobresia humilis in the habitat of Floccularia luteovirens,the diversity of endophytic fungi in K. humilis was studied by traditional isolation culture method and macrogenomic method. The results showed that：66 endophytic fungi were isolated from K. humilis and identified by traditional method,which belonged to Ascomycota and were classified into 7 orders,namely Pleosporales,Xylariales,Eurotiales,Helotiales and Hypocreales,Mucorales,Glomerellales. The dominant species were Stagonospora bicolor;the diversity index,richness index and evenness index of roots were 1.63,1.56 and 0.82,respectively. F. luteovirens was not obtained by isolation. 587 endophytic fungi were detected by macrogenomic method. Ascomycota accounted for 77.21%,Basidiomycota 16.21%,and the remaining 6.58% were unclassified fungi,the dominant population was Fusarium sp. The diversity index,richness index and evenness index of roots were 3.13,39.08 and 0.48,respectively. The F. luteovirens was detected and its relative abundance was 11.39. Comparing the two methods,there were abundant endophytic fungi resources in K. humilis in the habitat of F. luteovirens. F. luteovirens was one of the dominant endophytic fungi populations. This study proved for the first time that F. luteovirens was an endophytic fungus of Kobresia genus,it is a new breakthrough in studying the nutritional types of floccularia luteovirens and its interaction with plants.

Key words: Kobresia humilis, diversity of endophytic fungi, Floccularia luteovirens, traditional incubation method, metagenome method

郭璟, 谢占玲, 罗涛, 薛治峰, 郭建娟, 李发雄, 张秀娟. 黄绿卷毛菇生境中矮嵩草内生真菌多样性比较研究[J]. 生物技术通报, 2019, 35(11): 109-117.

GUO Jing, XIE Zhan-ling, LUO Tao, XUE Zhi-feng, GUO Jian-juan, LI Fa-xiong, ZHANG Xiu-juan. Comparative Study on Endophytic Fungi Diversity of Kobresia humilis in Floccularia luteovirens[J]. Biotechnology Bulletin, 2019, 35(11): 109-117.

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黄绿卷毛菇生境中矮嵩草内生真菌多样性比较研究

Comparative Study on Endophytic Fungi Diversity of Kobresia humilis in Floccularia luteovirens

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