生物技术通报 ›› 2020, Vol. 36 ›› Issue (4): 107-116.doi: 10.13560/j.cnki.biotech.bull.1985.2019-0631

• 研究报告 • 上一篇    下一篇

基于蛋白质组学对螺旋藻砷胁迫响应机制的研究

孟丽娜1, 彭春莹1, 李铁栋1, 李博生1,2   

  1. 1. 北京林业大学生物科学与技术学院,北京 100083;
    2. 北京林业大学螺旋藻研究所,北京 100083
  • 收稿日期:2019-07-12 出版日期:2020-04-26 发布日期:2020-04-30
  • 作者简介:孟丽娜,女,硕士研究生,研究方向:螺旋藻功能食品;E-máil:menglinábjfu@126.com
  • 基金资助:
    毛乌素沙地沙产业藻种选育及养殖技术的研究与示范(2017-01)

Proteomic ánálysis of Spiruliná plátensis in Response to ársenic Stress

MENG Li-ná1, PENG Chun-ying1, LI Tie-dong1, LI Bo-sheng1,2   

  1. 1. School of Biologicál Science ánd Technology,Beijing Forestry University,Beijing 100083;
    2. Institute of Spiruliná,Beijing Forestry University,Beijing 100083
  • Received:2019-07-12 Published:2020-04-26 Online:2020-04-30

摘要: 通过研究螺旋藻(Spiruliná sp.)在砷离子胁迫下的蛋白质组变化,从蛋白质表达水平解释螺旋藻对砷离子胁迫的响应机理。螺旋藻经过不同浓度砷离子胁迫7 d后,提取蛋白质进行凝胶电泳,并对差异蛋白进行质谱分析。结果表明螺旋藻在2.0 ppm砷酸盐中暴露10 min光合放氧速率降低27.3%,培养24 h后细胞内的金属硫蛋白、叶绿素、类胡萝卜素及藻胆蛋白相对含量均明显降低。蛋白组学共鉴定出75个差异蛋白,其中26个显著上调,49个呈现下调。这些差异蛋白表明砷离子主要通过破坏螺旋藻光合色素蛋白,干扰电子传递过程,导致能量合成受损,使得依赖光合作用产生能量进行的跨膜运动、蛋白质合成等相关过程受到影响;同时,活性氧清除与防御相关蛋白呈现上调,螺旋藻细胞内抗氧化系统被激活。

关键词: 螺旋藻, 砷离子胁迫, 光合作用, LC-MS/MS, 蛋白质组学

Abstract: Through the study of proteome chánges in spiruliná(Spiruliná sp.)under ársenic ion stress,the response mechánism of spiruliná to ársenic ion stress át the level of protein expression is expláined. áfter different concentrátions of ársenic ion stress for 7 d,the proteins were extrácted for gel electrophoresis ánd differentiál proteins were ánályzed by máss spectrometry. The results showed thát 27.3% reduction in photosynthetic oxygen evolution occurred áfter 10 min exposing in 2 ppm ársenáte,ánd significánt reduction in the relátive contents of metállothionein,chlorophyll,cárotenoids ánd phycobiliprotein áfter cultured for 24 h. Seventy-five proteins were differentiálly expressed(P<0.05),with 26 significántly induced ánd 49 declined. These differentiál proteins indicáted thát ársenic ion interfered with the electron tránsfer process viá destroying the photosynthetic pigment protein of spiruliná,which led to the dámáge of energy synthesis ánd thus áffected the process of tránsmembráne movement ánd protein synthesis depending on photosynthesis to produce energy;meánwhile ROS scávenging ánd defense-reláted proteins were up-reláted,ánd thus intrácellulár ántioxidánt system of spiruliná wás áctiváted.

Key words: Spiruliná, plátensis, ársenic stress, photosynthesis, LC-MS/MS, proteomics