解磷菌JL-1对磷矿粉降解性能的研究

doi:10.13560/j.cnki.biotech.bull.1985.2019-1054

摘要/Abstract

摘要： 以不动杆菌（Acinetobacter indicus）JL-1为菌种,磷矿粉为磷源,测试解磷菌JL-1对磷矿粉的降解能力。通过单因素实验及响应面试验得到最优降解磷矿粉条件为：以葡萄糖为碳源,浓度为17 g/L,以质量比为1∶1的硫酸铵与酵母粉为氮源,浓度为1.2 g/L,磷矿粉添加量为2.0 g/L,温度为28℃,初始pH为7.0,接种量为1%,在此最优条件下检测动态解磷曲线,在60 h 时降解磷矿粉的效果最好,此时解磷量为4.65 μg/mL,溶磷率为2.34%,菌落数达到最高,为10.65 log（CFU/mL）,pH达到最低。砂培实验表明磷矿粉中溶解的有效磷的72.08%会被菌体直接释放出来,7.63%会储存在菌体细胞中,20.29%会被菌体同化。

关键词: 解磷菌, 不动杆菌JL-1, 磷矿粉, 解磷性能

Abstract: In this study,rock phosphate was used as the phosphorus source for testing the phosphate-solubilizing ability of Acinetobacter indicus JL-1. The single-factor experiments and response surface test were utilized to determine the optimal conditions for degrading rock phosphate,and the optimal phosphate solubilizing condition was as follows：17 g/L glucose as carbon source,1.2 g/L the compound nitrogen source of ammonium sulfate and yeast powder at a mass ratio of 1∶1,2.0 g/L rock phosphate powder,incubation temperature 28℃,pH 7.0,and inoculum size 1%. Under the optimal condition,the dynamic phosphate-solubilizing curve showed that the maximal solubilizing phosphate concentration was 4.65 μg/mL at 60 h,the phosphate-solubilizing rate was 2.34%,and the number of colonies reached the highest,which was 10.65 log（CFU/mL）,while the pH reached the lowest. The Shapei experiment demonstrated that 72.08% of the available phosphorus was directly released,7.63% was stored in the cells,and 20.29% was assimilated by the strain.

Key words: phosphate-solubilizing bacteria, Acinetobacter indicus JL-1, rock phosphate, characteristics of phosphate solubilization

李文, 王陶. 解磷菌JL-1对磷矿粉降解性能的研究[J]. 生物技术通报, 2020, 36(8): 34-44.

LI Wen, WANG Tao. The Characteristics of Phosphate Solubilization of Rock Phosphate by Phosphate-solubilizing Bacterium JL-1[J]. Biotechnology Bulletin, 2020, 36(8): 34-44.

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